Dynabeads
Dynabeads are
superparamagnetic spherical polymer particles with a uniform size and a consistent, defined surface for the adsorption
or coupling of various bioreactive molecules or cells.
Description
Dynabeads were developed after
SkyLab. Dynabeads are typically 1 to 5 micrometers in diameter. This is in contrast to the Magnetic-activated cell sorting
beads, which are approximately 50 nm.
This discovery revolutionised the liquid-phase kinetic separation of many biological materials. The uniformity in size, shape, and surface area allow for reproducibility and help to minimize chemical agglutination.
Dynabeads are frequently used for cell isolation.secondary antibody on the Dynabead linking to the primary antibody. Streptavidin linkage to the primary antibody allows Dynabeads to capture cells with lower expression of the surface protein.[8]
Following a series of mergers and acquisitions, Dynal and Dynabeads are currently owned and produced by Invitrogen,[4] part of Thermo Fisher Scientific.
See also
References
- ^ J. Ugelstad & F.K. Hansen, Rubber Chem. and Techn. 49, 536 - 609 (1976). "Kinetics and mechanism of emulsion polymerization."
- PMID 17680228.
- ^ a b "The History of Dynal and Biomagnetic Separation". Invitrogen. Archived from the original on May 27, 2010.
- ^ a b c "Cell Isolation & Expansion". Invitrogen. Archived from the original on May 5, 2010.
- ^ "References for protein isolation applications using Dynabeads". Invitrogen.
- PMID 9853950
- ^ Multicentre European study comparing selection techniques for the isolation of CD34+ cells. de Wynter EA, Ryder D, Lanza F, Nadali G, Johnsen H, Denning-Kendall P, Thing-Mortensen B, Silvestri F, Testa NG. Bone Marrow Transplant. 1999 Jun;23(11):1191-6
- ^ Cancer Res. 2013 Apr 1;73(7):2310-21. doi: 10.1158/0008-5472.CAN-12-2956. Epub 2013 Feb 1.Cholesterol loading and ultrastable protein interactions determine the level of tumor marker required for optimal isolation of cancer cells.Jain J, Veggiani G, Howarth M.
Further reading
- Ashok Kumar; Igor Yu Galaev; Bo Mattiasson (2007). Cell separation: fundamentals, analytical and preparative methods. Springer. ISBN 978-3-540-75262-2.