Indirect immunoperoxidase assay

Microtiter plates with 96, 384 and 1536 wells

Indirect immunoperoxidase assay (IPA) is a laboratory technique used to detect and

classical plaque assays. These viruses include human coronavirus 229E and OC43.^[1]

Methodology

Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96-well plate. After viral growth, viral detection by IPA yields the infectious virus titer, expressed as

human cytomegalovirus.^[1]^[2]

References

^
PMID 19057861
.

^ G Gerna, C J McCloud and R W Chambers: Immunoperoxidase technique for detection of antibodies to Human cytomegalovirus. J. Clin. Microbiol. 1976, 3(3):364.

External links

Lambert, Francine; Jacomy, Helene; Marceau, Gabriel; Talbot, Pierre J. (28 April 2008). "Titration of Human Coronaviruses Using an Immunoperoxidase Assay". Journal of Visualized Experiments (14): e751.
PMID 19066576
. Retrieved 15 September 2016.

Immunohistochemistry Protocols, Buffers and Troubleshooting

[Lambert-1] 
PMID 19057861
.

[2] G Gerna, C J McCloud and R W Chambers: Immunoperoxidase technique for detection of antibodies to Human cytomegalovirus. J. Clin. Microbiol. 1976, 3(3):364.

[1]

[2]

v t e Medical tests used in immunology (CPT 86000–86849)
Immunoprecipitation	Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis
Immunoassay	Chemiluminescent immunoassay ELISA ELISpot Enzyme multiplied immunoassay technique RAST test Radioimmunoassay Radiobinding assay Immunofluorescence
Agglutination	Hemagglutination/Hemagglutinin Coombs test Latex fixation test
Other	Diagnostic immunology Nephelometry Complement fixation test Immunocytochemistry Immunohistochemistry Direct fluorescent antibody Epitope mapping Skin allergy test Patch test Total complement activity MELISA

Methodology

See also

References

External links