Percoll
erythrocytes by discontinuous density gradient centrifugation in Percoll.[1]
Percoll is a tool for efficient
silica particles of 15–30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone
(PVP).
Percoll is well suited for density gradient experiments because it possesses a low viscosity compared to alternatives, a low osmolarity, and no toxicity towards cells and their constituents.
Percoll is a registered trademark of
GE Healthcare
.
Characteristics
Percoll does not notably diffuse over time, resulting in the formation of very stable gradients. Therefore, both discontinuous and continuous Percoll density gradients can be prepared weeks in advance, allowing great reproducibility & ease of use.[3]
Past use in artificial reproduction
Percoll was previously used in
colloids in the ART industry.[4]
References
- PMID 6177116.
- .
- ^ "Cell separation media". Cytiva. Retrieved 24 June 2023.
- PMID 10819443. Retrieved 5 May 2010.