Rhodoplanes azumiensis

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Rhodoplanes azumiensis
Scientific classification
Domain:
Bacteria
Phylum:
Pseudomonadota
Class:
Alphaproteobacteria
Order:
Hyphomicrobiales
Family:
Nitrobacteraceae
Genus:
Rhodoplanes
Species:
R. azumiensis
Binomial name
Rhodoplanes azumiensis
Hiraishi 2017

Rhodoplanes azumiensis is a thermotolerant

bacterium isolated from sediment mud and cyanobacterial mats located in Nakanoyu hot springs, located in the Azumi district, Matsumoto, Nagano Prefecture, Japan. R. azumiensis is a photoheterotroph that is capable of using many organic materials as its carbon source. Colonies and liquid cultures exhibit a brownish red color when grown phototrophically, and are colorless when grown aerobically in darkness.[1]

Isolation

Samples were taken from sediment mud and cyanobacterial mats located in Nakanoyu hot springs in Japan. The samples were then introduced to 20 mL screw-capped test tubes containing 10-mL of Rhodoplanes (RPL) medium and were incubated at 42°C. Pink cultures occurred and were plated on RPL agar plates and incubated aerobically at 42°C. Isolation streaking was conducted multiple times until 2 novel species were isolated.

DNA sequencing and confirmation of novelty

20 strains of

DNA-DNA hybridization
was then conducted to determine whether or not these two isolated species were novel. Rhodoplanes azumiensis hybridized between 48 and 52% with previously isolated Rhodoplanes species allowing the researchers to determine it was indeed a novel strain.

Characteristics

R. azumiensis are

malonate as carbon sources
.

References

  1. PMID 29034848
    .
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