Ribonuclease V1

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Not to be confused with Ribonuclease V.
A Caspian cobra

Ribonuclease V1 (RNase V1) is a

nucleotides.[2] Like many ribonucleases, the enzyme requires the presence of magnesium ions for activity.[3]

Laboratory use

Purified RNase V1 is a commonly used

secondary structure or to perform footprinting experiments on macromolecular complexes containing RNA.[3]

RNase V1 is the only commonly used laboratory RNase that provides positive evidence for the presence of double-stranded helical conformations in target RNA.

base-paired but single-stranded,[5] dual susceptibility to both RNase V1 and RNase I at a single site in a target RNA molecule provides evidence of this relatively unusual conformation found in RNA loops.[6]

double helices
separated by flexible loops

Structural discoveries

RNase V1 played a particularly important role in the elucidation of the distinctive

deep sequencing.[11][12]

References

  1. ^
    PMID 7011369
    .
  2. ISBN 9781597451239
    .
  3. ^
    PMID 23547152
    .
  4. ISBN 9783110284959
    .
  5. PMID 2420800
    .
  6. PMID 24520115
    .
  7. PMID 7031604
    .
  8. PMID 9311812
    .
  9. PMID 19004957
    .
  10. PMID 1602537
    ..
  11. PMID 20811459
    .
  12. ISBN 978-3-319-29071-3
    .
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