Toeprinting assay
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The toeprinting assay, also known as the primer extension inhibition assay,ribosomes or RNA-binding proteins.[2] It is different from the more commonly used DNA footprinting assay. The toeprinting assay has been utilized to examine the formation of the translation initiation complex.[3]
To do a toeprint assay, one needs the mRNA of interest, ribosomes, a DNA
reagents.[4] The assay involves letting the RT generate cDNA until it gets blocked by any bound ribosomes, resulting in shorter fragments called toeprints when the results are observed on a sequencing gel
.