5-Bromo-4-chloro-3-indolyl phosphate

Source: Wikipedia, the free encyclopedia.
BCIP
Skeletal formula of BCIP
Ball-and-stick model of the BCIP molecule
Names
Preferred IUPAC name
5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate
Other names
BCIP
Identifiers
3D model (
JSmol
)
ChemSpider
UNII
  • InChI=1S/C8H6BrClNO4P/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14/h1-3,11H,(H2,12,13,14) ☒N
    Key: QRXMUCSWCMTJGU-UHFFFAOYSA-N ☒N
  • InChI=1/C8H6BrClNO4P/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14/h1-3,11H,(H2,12,13,14)
    Key: QRXMUCSWCMTJGU-UHFFFAOYAN
  • C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br
Properties
C8H6BrClNO4P
Molar mass 326.47 g·mol−1
Appearance Colorless
soluble in water (sodium salt)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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5-Bromo-4-chloro-3-indolyl phosphate (BCIP, X-phosphate, XP) is an artificial chromogenic

secondary antibodies
.

References

  1. ^ "5-Bromo-4-chloro-3-indolyl phosphate disodium salt". Millipore Sigma.
  2. ^ Alkaline phosphatase hydrolyses BCIP to 5-bromo-4-chloro-3-indoxyl<JP Horwitz J. Med. Chem., 1966, 9 (3), pp 447–447 and inorganic phosphate