Acid-growth hypothesis
The acid-growth hypothesis is a theory that explains the expansion dynamics of
History and development of the theory
Early development
Emergence of theory
Auxin was known to be a growth stimulant, but it was not until the year 1971 that Hager and Cleland proposed the "acid-growth hypothesis," which primarily suggested the correlation between auxin and apoplast acidification.
- In auxin-treated coleoptile and stem (hypocotyl) sections, auxin induces proton extrusion into the apoplast, which could decrease the pH value by as much as one full unit.
- Infiltration of neutral buffer (pH~7) into the apoplast could inhibit auxin-induced elongation and growth.
- Acidic buffers of pH 5.0 could accelerate cell elongation at the same or even greater rate in comparison to that induced by auxin.
- Fusiococcin (Fc) could also induce rapid cell elongation and growth, despite its primary role in promoting extensive acidification of the apoplast.
Constraints and interpretation
Within the 20-year timespan, many scientists have actively contributed to examining and reevaluating Hager's acid-growth hypothesis. Despite the accumulation of observations that evidently identify the final target of the auxin-induced action to be H+-ATPase, which excretes H+ protons to the apoplast and take in K+ ions through its rectifying K+ channel in the following years, the controversy has been carried over till today as an ongoing debate.[9]
Ongoing development
Discovery of hydrolytic enzyme
With H+ protons being excreted to the apoplast as one of the wall-loosening factors (WLF), scientists believed that the mechanism involves activation of
Transcriptional control
Transcriptional modification is crucial in the growth and development of cells When a plant is treated with auxin treatment, auxin-induced transcriptional changes occurs within minutes, which indicates that both
Modern interpretation
This acid-growth model has been updated to account for new mechanistic understanding. The decrease in apoplast pH value leads to cell-wall modification; the resulting increased extensibility of cell wall results in cell growth.[15] The reduction in apoplastic pH is mediated by auxin-induced mechanisms. With auxin acting as the primary signalling tool, it initiates apoplastic acidification via two mechanisms. Auxin stimulates the activity of the plasma-membrane proton pump (H+-ATPase), acidifying the wall. Auxin changes the cell wall's composition directly by increasing the transcription of wall-modifying agents.
Unsolved questions
There are several aspects of the theory that remain contentious. These include:
- Measurement of pH value: Despite optimal high-sensitivity detection brought by the implementation of fluorescent pH sensors, there is a lack of reliable method in quantifying the absolute apoplast pH value in plants. For instance, scientists made use of whole-organ resolution as part of their apoplastic pH measurement.[16] However, such research methods on the cellular level wouldn't establish equivalent validity at the quantitative level due to the plausible leaking of the signal from the endomembrane system into those originating from the apoplast.[17]
- Limitations on particular plant organs: Most of the research has been limited to the aerial organs of plants. Instead of studying the growth-promoting effect that auxin brings to the aerial organs, David Pacheco Villalobos discovers the inhibitory effect auxin has on root elongation.[18]
- Narrow coverage of plant species: Data that supported the early development of the theory initially originated from coleoptiles, epicotyls, and hypocotyls of a wide range of monocot and dicot species. To note, it all began with observations from sunflower.[1] The importance to expand the research territory beyond angiosperm species to more ancient members within the plant family tree is undeniable. Latter observations on different plant species could help identify conserved hormones and genes, or underlying mechanisms that support the theory, with the confirmation of SAUR19's role in auxin-induced hypocotyl elongation in tomatoes being one of the more recent discoveries.[19][20]