DecisionDx-UM

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DecisionDx-UM is a

choroidal melanoma, ciliary body melanoma, and iris melanoma. The DecisionDx-UM test was clinically validated on these tumors of the uveal tract. DecisionDx-UM assesses the gene expression profile (GEP) of a subset of genes which are differentially expressed in primary tumor cells compared to cells that have undergone transformation to a metastatic
phenotype.

The test classifies tumors as:

Also referred to as the gene expression profile, the test has been directly compared to all other

Biosciences
, Incorporated.

Uveal Melanoma

While rare, uveal melanoma (ocular melanoma) is the most common form of eye cancer and the second most common form of

proton beam irradiation) or eye removal (enucleation). Both are highly effective with ‘cure’ rates above 90%. However, as has been well documented in multiple publications including the Collaborative Ocular Melanoma Study (COMS), in nearly 50% of patients the melanoma has likely already metastasized by the time the primary eye tumor has been diagnosed and treated.[3][4] The majority of these metastases are microscopic and clinically undetectable at the time of primary tumor diagnosis.[5] Unfortunately, many of the current diagnostic techniques (including imaging and liver function tests) are not able to accurately detect micrometastases until tumor nodules have grown considerably, and likely become resistant to therapy.[6][7]

The lack of accurate prognostic tests to identify which patients are at risk of developing clinically overt metastatic disease has been a significant issue in the management of individual patients with uveal melanoma. Without accurate measures of individual metastatic risk, it is impossible to identify which patients are at high risk for clinical metastasis, which can then lead to either over or under-treatment of any given patient. The DecisionDx-UM test was developed to provide improved individual metastatic risk assessment which allows for more appropriate surveillance and treatment planning.

DecisionDx-UM GEP Assay

Discovery

The gene expression profile measured by DecisionDx-UM was discovered in the laboratory of J. William Harbour at Washington University in St. Louis.[8][9] The application of microarray analysis to primary untreated uveal melanomas revealed that the tumors clustered into two discreet groups based upon their expression profiles.[8] Filtering of the differentially expressed gene set led to the identification of a set of genes able to discriminate between Class 1 and Class 2 tumors.

In subsequent experiments, Dr. Harbour's group assembled microarray information from a larger subset of uveal melanoma tumor samples, and were able to identify candidate discriminatory genes for further analysis.

quantitative polymerase chain reaction (qPCR) methods, and predictive modeling was performed to identify an optimum set of genes able to accurately predict metastatic risk and report class assignments.[9][10] The final assay evaluated 12 discriminating genes and 3 control genes to determine Class 1 (low risk) and Class 2 (high risk) tumors, and was validated in a 609 patient multicenter study that included FNAB and FFPE samples.[9]

Prognostic Accuracy

The current DecisionDx-UM platform was clinically validated in the COOG study, which included 514 patients with UM treated at 12 ocular oncology centers in the United States between 1998 and 2010.[1] The report provides data showing that DecisionDx-UM has a high technical success rate (>95%).

Clinical Technical Success

Since the initial offering of DecisionDx-UM in December 2009, more than 800 clinical orders have been received for analysis by Castle Biosciences from over 55 ocular oncology centers across the U.S. Technical success and class determination was achieved for 96.2% of the samples.[11] Most of the failures were due to improper biopsy collection and/or handling. Tumor biopsy collection is critical for the successful reporting of metastatic risk Class assignment following DecisionDx-UM testing.

DecisionDx-UM Ordering and Reporting

Castle Biosciences, Incorporated only accepts service orders for DecisionDx-UM from licensed physicians (most commonly ocular oncologists, ophthalmologists, or retina specialists) who care for patients diagnosed with uveal melanoma. Physicians who are first time customers must contact Castle's for information and to establish an account. Sample submission requires submission of a DecisionDx-UM Requisition Form to be completed and faxed to Castle. FNAB specimens require use of a specimen collection kit, provided by Castle, to stabilize the sample and reduce the chance of technical failure. This kit must be received by the ordering physician prior to sample collection. Castle staff is available to assist with logistics concerning sample collection and shipping.

Castle provides a report to the ordering physician that will classify the tumor as Class 1A, Class 1B, or Class 2. The report will also describe the statistics associated with the classification, and provide a discriminant value that reflects the confidence regarding the classification. The information provided in the report from Castle identifies how likely a tumor is to metastasize (spread) with this risk being categorized as low (Class 1A), intermediate (Class 1B), or high (Class 2).

Other UM Metastasis Prognostic Factors

Histopathologic Factors and Imaging Techniques

Historically there are several clinical and pathologic features that have been statically associated with metastasis.

vascular structure have all been reported to correlate with metastatic onset and patient survival.[2][12][13][14]
While generalities regarding the UM patient population at large can be made based on these factors, none, alone or collectively, has demonstrated predictive accuracy with regard to metastatic activity.

Imaging techniques used to detect metastatic activity also have limitations. Systemic metastases are detected by

micrometastases
that may be present at the time of primary diagnosis of uveal melanoma.

Genetic Factors

Genetic analysis techniques have led to the identification of chromosomal abnormalities associated with metastatic tumor progression in uveal melanoma. Loss of

single nucleotide polymorphisms (SNP) have been associated with uveal melanoma metastatic lesions.[16][17][18][19][20] In particular, chromosomes 1, 3, 6 and 8 have been extensively studied for their association with shorter survival time and development of metastatic disease.[17][19][20][21][22][23][24]

While genetic aberrations in chromosomes 1, 6 and 8 have not independently been able to predict disease progression, cytogenetic profiling of chromosome 3 has uncovered an important genetic region highly associated with metastatic onset in uveal melanoma patients.

microsatellite analysis.[16][21][25][26][27][28]

Despite a wealth of data that has identified the association of chromosome 3 mutation with UM metastasis, the clinical application of this data has yet to be realized.[29] Reasons for the uncertainty of chromosome 3 clinical use include:

  • high false-positive rates (ranging from 5-22%)
  • high false-negative rates (ranging from 4-50%)
  • high technical failure rates (up to 50%) due to the large tissue requirement for current analysis techniques
  • the adverse effects of cellular
    heterogeneity on chromosome 3 cytogenetic analysis.[2][26][29][30][31][32][33][34]

Furthermore, unlike the DecisionDx-UM test, there are no published, peer-reviewed studies that demonstrate clinical validation in an independent set of patients.

Castle Biosciences, Incorporated

Castle Biosciences, Inc.
Nasdaq: CSTL

Castle Biosciences, Incorporated is a molecular diagnostics company that develops diagnostic and prognostic assays for rare cancers. Castle's mission is to serve individuals afflicted with rare cancers by offering accurate prognostic tests which provide information for making critical decisions regarding an individual's surveillance and treatment regimens. In addition to DecisionDx-UM, Castle offers prognostic tests for

Phoenix, AZ
.

References

  1. ^ a b Harbour, J.W., Retina Times 2011;29: 36-7.
  2. ^ a b c Worley, L.A., et al., Clinical Cancer Research, 2007. 13(5): p. 1466-71.
  3. ^ Archives of Ophthalmology, 2006. 124(12): p. 1684-93.
  4. ^ Diener-West, M., et al., Archives of Ophthalmology, 2001. 119(7): p. 969-82.
  5. ^ Harbour, J.W., Clinical overview of uveal melanoma:introduction to tumors of the eye., ed. D.M. Albert, Polans, A.2003: Marcel Dekker. 1-18.
  6. ^ a b Finger, P.T., et al., The British Journal of Ophthalmology, 2005. 89(10): p. 1270-4.
  7. ^ Kurli, M., et al., American Journal of Ophthalmology, 2005. 140(2): p. 193-9.
  8. ^ a b Onken, M.D., et al., Cancer research, 2004. 64(20): p. 7205-9.
  9. ^ a b c d Onken, M.D., et al., The Journal of molecular diagnostics : JMD, 2010. 12(4): p. 461-8.
  10. ^ Onken, M.D., et al., The Journal of molecular diagnostics : JMD, 2006. 8(5): p. 567-73.
  11. ^ Bergstrom, C.S., Hovland, P. G., Stone, J. F., Oelschlager, K., and Cook, R. W., 2011.
  12. ^ a b Augsburger, J.J. and J.W. Gamel, Cancer, 1990. 66(7): p. 1596-600.
  13. ^ a b McLean, I.W., K.S. Keefe, and M.N. Burnier, Ophthalmology, 1997. 104(5): p. 777-80.
  14. ^ Foss, A.J., et al., Cancer research, 1996. 56(13): p. 2900-3.
  15. ^ Servois, V., et al., European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 2010. 36(2): p. 189-94.
  16. ^ a b c Harbour, J.W., et al., Science, 2010. 330(6009): p. 1410-3.
  17. ^ a b Kilic, E., et al., Investigative ophthalmology & visual science, 2006. 47(9): p. 3703-7.
  18. ^ Onken, M.D., L.A. Worley, and J.W. Harbour, Clinical Cancer Research, 2008. 14(12): p. 3737-45.
  19. ^ a b Prescher, G., N. Bornfeld, and R. Becher, Journal of the National Cancer Institute, 1990. 82(22): p. 1765-9.
  20. ^ a b Sisley, K., et al., Genes, Chromosomes & Cancer, 1990. 2(3): p. 205-9.
  21. ^ a b Damato, B., J.A. Dopierala, and S.E. Coupland, Clinical Cancer Research, 2010. 16(24): p. 6083-92.
  22. ^ Sisley, K., et al., Genes, Chromosomes & Cancer, 1997. 19(1): p. 22-8.
  23. ^ a b Prescher, G., et al., Lancet, 1996. 347(9010): p. 1222-5.
  24. ^ Singh, A.D., et al., Archives of Pathology & Laboratory Medicine, 2009. 133(8): p. 1223-7.
  25. ^ Naus, N.C., et al., Clinical Cancer Research, 2002. 8(2): p. 534-9.
  26. ^ a b Onken, M.D., et al., Clinical Cancer Research, 2007. 13(10): p. 2923-7.
  27. ^ Sandinha, M.T., M.A. Farquharson, and F. Roberts, The British Journal of Ophthalmology, 2004. 88(12): p. 1527-32.
  28. ^ Tschentscher, F., et al., Cancer genetics and cytogenetics, 2000. 122(1): p. 13-7.
  29. ^ a b Damato, B. and S.E. Coupland, Archives of Ophthalmology, 2009. 127(4): p. 423-9.
  30. ^ Maat, W., et al., Archives of Pathology & Laboratory Medicine, 2007. 131(1): p. 91-6.
  31. ^ Mensink, H.W., et al., Investigative ophthalmology & visual science, 2009. 50(2): p. 500-4.
  32. ^ Midena, E., et al., European journal of ophthalmology, 2006. 16(3): p. 422-5.
  33. ^ Shields, C.L., et al., Transactions of the American Ophthalmological Society, 2007. 105: p. 43-52; discussion 52-3.
  34. ^ Young, T.A., et al., Ophthalmology, 2007. 114(1): p. 142-6.

External links

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