Mitotic recombination

Source: Wikipedia, the free encyclopedia.

Mitotic recombination is a type of

tumorigenesis and lethal recessive alleles.[1][2]
Mitotic homologous recombination occurs mainly between sister chromatids subsequent to replication (but prior to cell division). Inter-sister homologous recombination is ordinarily genetically silent. During mitosis the incidence of recombination between non-sister homologous chromatids is only about 1% of that between sister chromatids.[3]

Discovery

The discovery of mitotic recombination came from the observation of twin spotting in Drosophila melanogaster. This twin spotting, or mosaic spotting, was observed in D. melanogaster as early as 1925, but it was only in 1936 that Curt Stern explained it as a result of mitotic recombination. Prior to Stern's work, it was hypothesized that twin spotting happened because certain genes had the ability to eliminate the chromosome on which they were located.[4] Later experiments uncovered when mitotic recombination occurs in the cell cycle and the mechanisms behind recombination.

Mitotic recombination can result in homozygous expression in a heterozygous individual[dead link]

Occurrence

Mitotic recombination can happen at any locus but is observable in individuals that are heterozygous at a given locus. If a crossover event between non-sister chromatids affects that locus, then both homologous chromosomes will have one

metaphase plate
. If the chromatids containing different alleles line up on the same side of the plate, then the resulting daughter cells will appear heterozygous and be undetectable, despite the crossover event. However, if chromatids containing the same alleles line up on the same side, the daughter cells will be homozygous at that locus. This results in twin spotting, where one cell presents the homozygous recessive phenotype and the other cell has the homozygous wild type phenotype. If those daughter cells go on to replicate and divide, the twin spots will continue to grow and reflect the differential phenotype.

Mitotic recombination takes place during interphase. It has been suggested that recombination takes place during G1, when the DNA is in its 2-strand phase, and replicated during DNA synthesis.[5] It is also possible to have the DNA break leading to mitotic recombination happen during G1, but for the repair to happen after replication.[6][7]

Response to DNA damage

In the budding yeast Saccharomyces cerevisiae, mutations in several genes needed for mitotic (and meiotic) recombination cause increased sensitivity to inactivation by radiation and/or genotoxic chemicals.[8] For example, gene rad52 is required for mitotic recombination[9] as well as meiotic recombination.[10] Rad52 mutant yeast cells have increased sensitivity to killing by X-rays, methyl methanesulfonate and the DNA crosslinking agent 8-methoxypsoralen-plus-UV light, suggesting that mitotic recombinational repair is required for removal of the different DNA damages caused by these agents.

Mechanisms

The mechanisms behind mitotic recombination are similar to those behind meiotic recombination. These include sister chromatid exchange and mechanisms related to DNA double strand break repair by

SDSA), and gene conversion through a double-Holliday Junction intermediate or SDSA. In addition, non-homologous mitotic recombination is a possibility and can often be attributed to non-homologous end joining.[6][7][11][12]

Method

There are several theories on how mitotic crossover occurs. In the simple crossover model, the two homologous chromosomes overlap on or near a common

double-strand break,[13] which is then repaired using one of the two strands. This can lead to the two chromatids switching places. In another model, two overlapping sister chromatids
form a double Holliday junction at a common repeat site and are later sheared in such a way that they switch places. In either model, the chromosomes are not guaranteed to trade evenly, or even to rejoin on opposite sides thus most patterns of cleavage do not result in any crossover event. Uneven trading introduces many of the deleterious effects of mitotic crossover.

Alternatively, a crossover can occur during DNA repair

homozygous
chromosome.

Advantages and disadvantages

Mitotic crossover is known to occur in D. melanogaster, some asexually reproducing fungi and in normal human cells, where the event may allow normally recessive cancer-causing alleles to be expressed and thus predispose the cell in which it occurs to the development of

Bloom's syndrome is caused by a mutation in RecQ helicase, which plays a role in DNA replication and repair. This mutation leads to high rates of mitotic recombination in mice, and this recombination rate is in turn responsible for causing tumor susceptibility in those mice.[15] At the same time, mitotic recombination may be beneficial: it may play an important role in repairing double stranded breaks, and it may be beneficial to the organism if having homozygous dominant alleles is more functional than the heterozygous state.[2] For use in experimentation with genomes in model organisms such as Drosophila melanogaster, mitotic recombination can be induced via X-ray and the FLP-FRT recombination system.[16]

References

  1. ^ a b Hartl, Daniel L. and Maryellen Ruvolo (2012). Genetics: Analysis of Genetics and Genomes. Burlington: Jones & Bartlett.
  2. ^
    PMID 9345110
    .
  3. PMID 20177395
    .
  4. PMID 17246815
    .
  5. PMID 360220
    .
  6. ^
    PMID 19282969
    .
  7. ^
    PMID 19282976
    .
  8. ^ Haynes, R.H. & Kunz, B.A. (1981). DNA repair and mutagenesis in yeast. In: Strathern, J; Jones, E; Broach J. editors. The Molecular Biology of the Yeast Saccharomyces. Life Cycle and Inheritance. Cold Spring Harbor, N.Y., Cold Spring Harbor Laboratory, 371-414.
  9. PMID 6987653
    .
  10. PMID 17248996
    .
  11. PMID 14643432
    .
  12. PMID 10357855
    .
  13. ^ Helleday, T. "Double-Strand Break Repair via Double Holliday Junctions (Szostak Model)". Animation. MIT.
  14. PMID 14643432. Archived from the original
    (PDF) on 24 May 2014. Retrieved 2012-12-26.
  15. S2CID 21218975
    .
  16. PMID 8404527
    .
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