Nestin (protein)
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RefSeq (mRNA) |
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RefSeq (protein) |
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Location (UCSC) | Chr 1: 156.67 – 156.68 Mb | n/a | |||||||
PubMed search | [2] | n/a |
View/Edit Human |
Nestin is a protein that in humans is encoded by the NES gene.
Nestin (acronym for neuroepithelial stem cell protein) is a type VI
Gene
Structurally, nestin has the shortest head domain (N-terminus) and the longest tail domain (C-terminus) of all the IF proteins. Nestin is of high molecular weight (240kDa) with a terminus greater than 500 residues (compared to cytokeratins and lamins with termini less than 50 residues).[6]
After subcloning the human nestin gene into
Expression
Nestin is expressed by many types of cells during development, although its expression is usually transient and does not persist into adulthood. One instance of nestin expression in adult organisms, and perhaps that for which nestin is best known, are the neuronal precursor cells of the
Function
Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α-internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.[4]
Clinical significance
Nestin, a protein marker for
Nestin has recently received attention as a marker for detecting newly formed endothelial cells. Nestin is an angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.[9]
Interactions
Nestin (protein) has been shown to
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000132688 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- PMID 17854500.
- ^ PMID 15736068.
- PMID 10092680.
- ^ "About intermediate filaments". Cytoskeleton, Inc. Archived from the original on 2008-08-07. Retrieved 2008-08-16.
- PMID 1478958.
- S2CID 1039201.
- PMID 17273760.
- PMID 12832492.
Further reading
- Wiese C, Rolletschek A, Kania G, Blyszczuk P, Tarasov KV, Tarasova Y, Wersto RP, Boheler KR, Wobus AM (2004). "Nestin expression--a property of multi-lineage progenitor cells?". Cellular and Molecular Life Sciences. 61 (19–20): 2510–22. S2CID 2263546.
- Tiede S, Kloepper JE, Ernst N, Poeggeler B, Kruse C, Paus R (2009). "Nestin in human skin: exclusive expression in intramesenchymal skin compartments and regulation by leptin". Journal of Investigative Dermatology. 129 (11): 2711–20. PMID 19554024.
- Dahlstrand J, Zimmerman LB, McKay RD, Lendahl U (1992). "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments". Journal of Cell Science. 103 ( Pt 2) (2): 589–97. PMID 1478958.
- Hockfield S, McKay RD (1985). "Identification of major cell classes in the developing mammalian nervous system". Journal of Neuroscience. 5 (12): 3310–28. PMID 4078630.
- Lothian C, Lendahl U (1997). "An evolutionarily conserved region in the second intron of the human nestin gene directs gene expression to CNS progenitor cells and to early neural crest cells". European Journal of Neuroscience. 9 (3): 452–62. S2CID 34930077.
- Yaworsky PJ, Kappen C (1999). "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene". Developmental Biology. 205 (2): 309–21. PMID 9917366.
- Cassiman D, van Pelt J, De Vos R, Van Lommel F, Desmet V, Yap SH, Roskams T (1999). "Synaptophysin: A novel marker for human and rat hepatic stellate cells". American Journal of Pathology. 155 (6): 1831–9. PMID 10595912.
- Messam CA, Hou J, Major EO (2000). "Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody". Experimental Neurology. 161 (2): 585–96. S2CID 40226606.
- About I, Bottero MJ, de Denato P, Camps J, Franquin JC, Mitsiadis TA (2000). "Human dentin production in vitro". Experimental Cell Research. 258 (1): 33–41. S2CID 36709413.
- Sanchez-Ramos J, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Janssen W, Patel N, Cooper DR, Sanberg PR (2000). "Adult bone marrow stromal cells differentiate into neural cells in vitro". Experimental Neurology. 164 (2): 247–56. S2CID 6382107.
- Woodbury D, Schwarz EJ, Prockop DJ, Black IB (2000). "Adult rat and human bone marrow stromal cells differentiate into neurons". Journal of Neuroscience Research. 61 (4): 364–70. PMID 10931522.
- Akiyama Y, Honmou O, Kato T, Uede T, Hashi K, Kocsis JD (2001). "Transplantation of clonal neural precursor cells derived from adult human brain establishes functional peripheral myelin in the rat spinal cord". Experimental Neurology. 167 (1): 27–39. S2CID 730995.
- Sahlgren CM, Mikhailov A, Hellman J, Chou YH, Lendahl U, Goldman RD, Eriksson JE (2001). "Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase". Journal of Biological Chemistry. 276 (19): 16456–63. PMID 11278541.
- Messam CA, Hou J, Berman JW, Major EO (2002). "Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells". Brain Research. Developmental Brain Research. 134 (1–2): 87–92. PMID 11947939.
- Safford KM, Hicok KC, Safford SD, Halvorsen YD, Wilkison WO, Gimble JM, Rice HE (2002). "Neurogenic differentiation of murine and human adipose-derived stromal cells". Biochemical and Biophysical Research Communications. 294 (2): 371–9. PMID 12051722.
- Gu H, Wang S, Messam CA, Yao Z (2002). "Distribution of nestin immunoreactivity in the normal adult human forebrain". Brain Research. 943 (2): 174–80. S2CID 1689033.
- Vanderwinden JM, Gillard K, De Laet MH, Messam CA, Schiffmann SN (2002). "Distribution of the intermediate filament nestin in the muscularis propria of the human gastrointestinal tract". Cell and Tissue Research. 309 (2): 261–8. S2CID 34390115.
- Piper K, Ball SG, Turnpenny LW, Brickwood S, Wilson DI, Hanley NA (2002). "Beta-cell differentiation during human development does not rely on nestin-positive precursors: implications for stem cell-derived replacement therapy". Diabetologia. 45 (7): 1045–7. S2CID 28062847.
- About I, Mitsiadis TA (2001). "Molecular aspects of tooth pathogenesis and repair: in vivo and in vitro models". Advances in Dental Research. 15: 59–62. S2CID 2411089.
External links
- Nestin at the U.S. National Library of Medicine Medical Subject Headings (MeSH)