Talk:Jeffery Taubenberger

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I think the story of Johan Hultin is more interesting and more credit should be given him towards the discovery and re-sequencing of the 1918 virus.

Seems all the guts & glory have gone to this guy...

Juergen S. Philippines: I am study in Virology H1N1 so if you get any Information about Spanish Flu please let me know ! Thank you very much!

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Hi guys,

I'm not sure who's actually responsible for this, but the following section doesn't make sense to me: "The genome of the flu virus includes about 13,000 base pairs, which had decayed into pieces as small as 100 base pairs. In order to make PCR work, primers have to be constructed, i.e. a short bit of DNA with mirror sequences of the sequence at the two end points of the fragment. They bind to the fragment, and with the help of a polymerase bases are added between the primers to make a copy. The millions of copies of the gene segment are labelled with a radioactive probe as they are being made. They can then be separated on a thin gel by running an electric current across the gel. The radioactive labels create a black mark on an X-ray film which is put over the gel."

So whilst PCR (polymerase chain reaction) effectively fills in the gap between the two primers, it doesn't actually work like that. It's DNA replication, therefore each strand is extended from one of the two primers. As for labelling with a radioactive probe...kind of, that method of sequencing isn't used any more, but the technique is still valid. It's basically termination sequencing where each fragment terminates in a radioactive isotope, so in practice with lots of fragments of varying length you can read the sequence. BUT this is separate from PCR! It follows on from that, to sequence the fragment.

Hope this makes sense

Tim — Preceding unsigned comment added by 213.48.60.6 (talk) 12:40, 10 September 2018 (UTC)[reply]

Hello. I apologize for making an unsolicted edit, which was a question, if Dr. Carter had been the author of stranger than fiction. I was looking for the book mentioned and stumbled upon another person named Dr. Crane. Since my edit may have been wrong, since it was taken back almost immediately, I wanted to ask, if changes are taken back because of formatting, or because of further knowledge and other sources. And if there is a source regarding Dr. Carter being the real author, to give some reference. Thanks! 46.223.196.184 (talk) 21:26, 31 January 2022 (UTC)[reply]