Trizol
TRIzol is a widely used
TRIzol is the brand name of
which was founded by Chomczynski.Uses in extraction
The correct name of the method is
DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct-zol Miniprep kit by Zymo Research.[7] This method eliminates the use of Chloroform and 1-bromo-3-chloropropane completely, bypassing phase-separation and precipitation steps.[8]
TRIzol is light-sensitive and is often stored in a dark-colored, glass container covered in foil. It is stored at room temperature.
When used, it resembles cough syrup, bright pink. The smell of the phenol is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
Hazards
Vigilant caution should be taken while using TRIzol (due to the phenol and chloroform).
TRIzol is labeled as acute oral, dermal, and inhalation toxicity besides skin corrosion/irritation in the manufacturer MDS.[9]
Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns, permanent scarring and kidney failure.
Experiments should be performed under a chemical hood, with lab coat, nitrile gloves and a plastic apron. [10][11]
TRIzol waste should never be mixed with bleach or acids: the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases.
References
- ^ "Total RNA isolation: Trizol vs RNeasy". www.bioastrum.com. Retrieved 2021-08-18.
- PMID 2440339.
- ^ Life Technologies
- ^ Molecular Research Center, Inc.
- ^ "RNA isolation" (PDF). Cell Analysis Facility. Archived from the original (PDF) on 2021-08-18. Retrieved 2021-08-18.
- ^ "Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database". brd.nci.nih.gov. Retrieved 2021-08-18.
- ^ "Direct-zol RNA Microprep Kits".
- ^ "Nucleic acid purification".
- ^ "TRIZOl MDS" (PDF).
- ^ Material Safety data sheet from Life technologies. Archived 2008-07-21 at the Wayback Machine
- ^ Material Safety data sheet from MRC Archived 2008-12-28 at the Wayback Machine