RNA extraction

Source: Wikipedia, the free encyclopedia.

RNA extraction is the purification of

guanidinium thiocyanate-phenol-chloroform extraction.[2][3] The filter paper based lysis and elution method features high throughput capacity.[4]

RNA extraction in liquid nitrogen, commonly using a mortar and pestle (or specialized steel devices known as tissue pulverizers) is also useful in preventing ribonuclease activity.

RNase contamination

The extraction of RNA in molecular biology experiments is greatly complicated by the presence of ubiquitous and hardy RNases that degrade RNA samples. Certain RNases can be extremely hardy and inactivating them is difficult compared to neutralizing

RNase A superfamily, is secreted by human skin and serves as a potent antipathogen defence.[8][9] For these secreted RNases, enzymatic activity may not even be necessary for the RNase's exapted function. For example, immune RNases act by destabilizing the cell membranes of bacteria.[10][11]

To counter this, equipment used for RNA extraction is usually cleaned thoroughly, kept separate from common lab equipment and treated with various harsh chemicals that destroy RNases. For the same reason, experimenters take special care not to let their bare skin touch the equipment.

See also

References

  1. PMID 17417019
    .
  2. S2CID 28653075
    .
  3. PMID 16028681
    .
  4. ^ FortiusBio High Throughput RNA Extraction Filter Paper Card
  5. PMID 19246759
    .
  6. PMID 20189811
    .
  7. S2CID 20922592
    .
  8. PMID 12244054
    .
  9. PMID 19641608
    .
  10. PMID 17150966
    .
  11. PMID 18211964
    .


External links

Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits; by Erik Jue, Daan Witters & Rustem F. Ismagilov; Nature, Scientific reports, 2020.

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