Addgene
Non-profit organization, biological resource center | |
| Location | |
|---|---|
| Services | Plasmid repository |
Key people | Chonnettia Jones, Executive director |
Addgene is a non-profit
History
Addgene was founded in 2004 by Melina Fan, Kenneth Fan, and Benjie Chen.[1][2]
Operations
Addgene's headquarters are located in
Addgene accepts plasmids from researchers for distribution and archival.
The organization covers the operating costs of maintaining and improving the collection by charging a nominal fee to scientists requesting plasmids.[3][4]
Plasmid repository
As of 2014 Addgene's repository comprised 30,000 plasmids, deposited by 1,700 labs.
- Genome engineering
- CRISPRs
- Transcription Activator-Like Effector Nuclease(TALEN) kits
- Zinc finger nucleasekits
- Empty backbones
- Species-specific expression
- Epitope tags
- Fusion proteins
- Selectable markers
- Fluorescent marker
- Viral vectors
- Retroviral/Lentiviral
- Adenoviral
- AAV
- cDNAexpression
- shRNA expression
Tools and guides
Molecular biology tools
Vector Database—A curated list of over 4,000 vector backbones, including relevant cloning information and bacterial growth conditions.
Sequence Analyzer—An Addgene software tool for creating plasmid maps from sequences with annotated features and restriction sites.
Molecular Biology Reference—A collection of references for molecular biology reagents, such as primers, restriction enzymes and antibiotic concentrations.
Plasmid Cloning Guides
Molecular Cloning Guides—References to help scientists design plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.
Molecular Cloning Protocols—Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis and bacterial transformation.
Collaborations
Addgene collaborates with institutes and consortia to curate plasmid collections for specific purposes. Examples of these collaborations include special collections from the
In 2020, Addgene received funding from Fast Grants to subsidize the cost of reagents for COVID-19 research.[7]
Depositors
Noteworthy depositors include:[citation needed]
- 13 Nobel Prize winners; Roger Tsien, Jennifer Doudna, and Johann Deisenhofer.
- 8 Breakthrough Prize in Life Sciences winners; Cori Bargmann, David Botstein, Lewis C. Cantley, Hans Clevers, Titia de Lange, Bert Vogelstein, Robert Weinberg and Shinya Yamanaka.
Electronic Material Transfer Agreements
Addgene requires
Awards
Addgene won awards for innovation and research including Mass Nonprofit Network Award for excellence in Innovations,[9][10] Cambridge award program 2014 Award for Research & Development Laboratories,[11] Mass Technology Leadership Award Finalist 2012.[12]
References
- ^ Scudellari, Megan (2012). "Sharing Made Easy". The Scientist. Retrieved 21 November 2012.
- PMID 15790830.
- ^ Kamens, J (2012). "Got any plasmids?" (PDF). Lab Times. pp. 58–59. Retrieved 21 November 2012.
- ISSN 1476-4687.
- ^ Baker, Monya. "Repositories share key research tools". Nature.com. Retrieved 8 May 2014.
- ^ Resende, Patricia (3 October 2012). "Addgene partners with Michael J. Fox Foundation". Mass High Tech. Archived from the original on 9 October 2012. Retrieved 21 November 2012.
- ^ "Fast Grants". Fast Grants. Archived from the original on 2021-12-23. Retrieved 2023-05-21.
- PMID 22491276.
- ^ "mass nonprofit network nominates addgene for an excellence award" (PDF). Retrieved 9 May 2014.
- ^ "mass non-profit, nonprofit award 2013 excellence in innovation finalists". Archived from the original on 2013-09-02. Retrieved 9 May 2014.
- ^ "cambridge recognition awarding". Retrieved 9 May 2014.
- ^ "Mass Tech announces finalist". Archived from the original on 17 April 2014. Retrieved 9 May 2014.
