MCF-7

MCF-7 is a

Prior to MCF-7, it was not possible for cancer researchers to obtain a mammary cell line that was capable of living longer than a few months.[4]

The patient, Frances Mallon died in 1970. Her cells were the source of much of current knowledge about breast cancer.^[2][5] At the time of sampling, she was a nun in the convent of Immaculate Heart of Mary in Monroe, Michigan under the name of Sister Catherine Frances.

MCF-7 and two other breast cancer cell lines, named

MCF-7 cells have the following characteristics:[2]^{[5][6][7][8][9]}

• Primary tumor (invasive breast ductal carcinoma)
• Originate from pleural effusion
• Estrogen receptors present^[10]
• Proliferative response to
  estrogens
• Presence of progesterone receptors
• Cannot have
  Her2/neu
  protein overexpression)
• Tumorigenic in mice but only with estrogen supplementation if engrafted into the subcutaneous fat or mammary fat pad
• Tumorigenic in mice without estrogen supplementation if engrafted intraductally^[11]
• Luminal epithelial phenotype

This cell line retained several characteristics of differentiated mammary epithelium, including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes.^{[citation needed]}

Tumor necrosis factor alpha (

insulin-like growth factor binding proteins. Omega-3 and 6 fatty acids such as EPA, DHA and AA has been reported to inhibit MCF-7 cell line growth and proliferation.^[12]

PIK3CA helical mutations were identified in MCF-7,[13] but with low AKT activation.^[14]

References

1. PMID 25828948
   .
2. ^
   PMID 4357757
   .
3. ^ http://www.cancer.gov "NCI Cancer Bulletin for April 29, 2008 - National Cancer Institute". Archived from the original on 2010-05-27. Retrieved 2010-04-28. Retrieved on 2010-04-28
4. ^ Glodek, Cass, Ph.D., "A History of the Michigan Cancer Foundation, the Beginnings & Growth of Detroit's Anticancer Movement," 1990, page 68, Michigan Cancer Foundation, Detroit.
5. ^
   PMID 9242427
   .
6. ^
   S2CID 207628369
   .
7. PMID 11673656
   .
8. PMID 16288205
   .
9. PMID 16728565
   .
10. ^ Fanelli, Alex (2016). "MCF-7 Cells: human breast adenocarcinoma cell line". Retrieved 3 December 2017.
11. PMID 26947176
    .
12. PMID 26325577
    .
13. ^ Cosmic. "COSMIC: Sample overview for 1289391". cancer.sanger.ac.uk. Retrieved 2017-05-10.
14. PMID 19573809
    .

External links

Wikimedia Commons has media related to MCF-7 cells.

• The University of Texas MD Anderson Breast Cancer Cell Line Data Base
• Cellosaurus entry for MCF-7