Peanut agglutinin
Peanut agglutinin (PNA) is plant
on their surface which include the Gal-β(1-3)-GalNAc carbohydrate sequence.Structure
| Legume lectin domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
The protein is 273 amino acids in length with the first 23 residues acting as a signal peptide which is subsequently cleaved. It has a
tetrameric quaternary structure. It is a member of the Lectin_legB PFAM
family.
Available Structures of peanut agglutinin
Uses in cell biology and biochemistry
Because peanut agglutinin specifically binds a particular carbohydrate sequence it finds use in a range of methods for cell biology and biochemistry. For example in PNA-affinity chromatography the binding specificity of peanut agglutinin is used to isolate glycosylated molecules which have the sugar sequence Gal-β(1-3)-GalNAc. Peanut agglutinin activity is inhibited by lactose and galactose which compete for the binding site.
Other uses include:
- Potent anti-T cell activity.
- Distinguishing between human lymphocyte subsets.
- Identification of cone cell inner and outer segments and to a lesser extent rod cell inner segments in the mammalian retina.[3]
- mucosamalignancies.
- Identification of mammalian-infective metacyclic promastigote Leishmania major parasites from other life cycle forms also found in the sandflyhost.
- Identification of the outer acrosome membrane in sperm, indicating acrosome integrity. [4]
See also
References
- ^ "PNA specification sheet Medicago AB" (PDF). Retrieved 2010-03-14.[permanent dead link]
- S2CID 20037257.
- PMID 6715128.
- S2CID 23548329.
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