User:Cfb49840/Methanobacterium

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Methanobacterium, or methanogens, are a genus of the Methanobacteria class in the Archaea kingdom, which are known for their ability to produce methane as a metabolic byproduct.[1] Methane gas is known for being a fuel source, but also, a greenhouse gas, most notably known for implicating global warming.[2] Despite the name, this genus belongs not to the bacterial domain, but the to the archaeal domain because they lack peptidoglycan in their cell walls.[3] Methanogens, therefore are archae that produce methane. [4] Methanobacterium are nonmotile and live without oxygen.[5] They are incredibly sensitive to oxygen which means they strictly live in anoxic environments.[2] A shared trait by all methanogens is their ability to cycle products.[2] They can use the products of metabolic activities occurring during methanogenesis as substrates for the formation of methane.[2] Methanobacterium species typically thrive in environments with optimal growth temperatures ranging from 28 to 40 °C, and in versatile ecological ranges.[6] They are a part of the scientific world that is still relatively unknown, but methanogens are thought to be some of earth’s earliest life forms, with origins dating back over 3.4 billion years.[7] In summary, much is still unknown but over the course of many years, the scientific community has accumulated vast data regarding methanogen's history, microbiology, and much more.

History

The evolutionary history of Methanobacterium is still relatively unknown, but methanogens are thought to be some of earth’s earliest life forms, with methane origins dating back over 3.4 billion years.[7] They were always obtained via high dilution strategies, making it almost impossible to successfully isolate.[8] In 1776, Alesandro Volta discovered that gas bubbles coming from a freshwater swamp were flammable.[9] This finding lead him to propose the possibility that living organisms were producing methane gas. [9] However, many concluded the methane gas was a result of decomposing organic matter.[9] In 1993, methanogens were first cultured and it was revealed that this methane was coming from living organisms.[9] In 1966, a strain named Methanobacterium formicicum was cultured and isolated from a sewage sample collected in Urbana, Illinois. [8] The archae was present at high densities in anaerobic digestors and freshwater sediments.[8] In this report, the cells were shaped like long crooked rods, forming rod chains and filaments.[8]

Microbiology

Structure

Methanobacterium are generally

microbes.[10] Because there are many different species in the Methanobacterium genus, there are a variety of shapes, sizes, and arrangements these microbes can possess.[11] These rod shaped microbes can be curved, straight, or crooked.[10] They can also range in size; some can be short or long, and can be found individually, in pairs, or in chains.[11] Some Methanobacterium species can even be found in large clusters or aggregates consisting of long intertwined chains of individual microbes.[12]

There have been many strains of Methanobacterium that have been isolated and studied profoundly. One in particular, Methanobacterium thermoautotrophicum, revealed the presence of intracytoplasmic membranes, an internal membrane system consisting of 3 membranes stacked on top of each other without a cytoplasm separating them

pseudomurein,[15] a carbohydrate backbone and a cross-linking peptide with amino acids that form the peptide bonds and serve the nature of the bonding and sugar type.[16]

Physiology

Methanobacterium are strict anaerobes, meaning they cannot survive in the presence of oxygen.[10] They are ubiquitous in hot, low-oxygen environments, such as anaerobic digesters, wastewater, and hot springs.[17] Most species belonging to this genus are also autotrophs, which create organic compounds from inorganic materials such as carbon dioxide.[11] Methanobacterium can be classified as hydrogenotrophic methanogens.[11] Hydrogenotrophic methanogens use hydrogen, carbon dioxide, formate, and alcohols to synthesize methane.[11] These substrates are also important for the growth and maintenance of Methanobacterium.[11]

Mutagenesis is a vital part of the carbon cycle as it performs the conversion of organic carbon into methane gas.

secondary alcohols.[2] Some members of this genus can use formate to reduce methane; others live exclusively through the reduction of carbon dioxide with hydrogen.[4]

Genome

Researchers have been able to sequence the genome of seven different Methanobacterium and

pseudomurein cell wall, propose that the species is capable of inhibition by the small molecule inhibitor and vaccine. [19] This is determined by the methane alleviation devices that have the ability to grow the genes found in the rumen.[19]

Methanobacterium plays a role in both the waste and

alpha subunit, shows that there are additional unidentified methanogens that exist in other ecosystems.[20]

Bacterial Essentials: Growth and Survival

Optimal Growth Temperature

Methanobacterium species typically thrive in environments with optimal growth temperatures ranging from 28 to 40°C.[6] Methanobacteria are widely distributed in geothermal settings like hot springs and hydrothermal vents.[21] This mesophilic temperature range indicates that Methanobacterium organisms are adapted to moderate environmental conditions, neither extremely hot nor cold.[22] This temperature preference allows them to inhabit a variety of anaerobic environments, including soil, sediments, and animal digestive tracts, where conditions often fall within this mesophilic range.[6] Within these habitats, Methanobacterium species contribute to methane production through their hydrogenotrophic metabolism, utilizing hydrogen and carbon dioxide as metabolic substrates.[6]

Habitat

Methanobacterium species inhabit various anaerobic environments, showcasing a versatile ecological range.[10] They can be found in diverse habitats such as soil, wetlands, sediment layers, sewage treatment plants, and the gastrointestinal tracts of animals.[6] Within these environments, Methanobacterium species play crucial roles in anaerobic microbial ecosystems, contributing to processes like organic matter decomposition via methane production through the methanogenesis pathway.[23]

Diversity and Taxonomy

Methanogens, including Methanobacterium, belong to the archaea domain, characterized by unique features such as unconventional 16S rRNA sequences, distinct lipid structures, and novel cell wall compositions.

psychrotrophic to hyperthermophilic, and varying salinity preferences from freshwater to saturated brine.[4] Despite their taxonomic placement within archaea, methanogens display diverse cellular envelopes, which can consist of protein surface layers (S-layers), glycosylated S-layer proteins, additional polymers like methanochondroitin, or pseudomurein in Gram-positive staining species.[4] Methanogens are unique among archaea in their adaptability to a broad spectrum of environmental conditions, with a preference for neutral to moderately alkaline pH values.[4]

Taxonomically, methanogens are classified into 25 genera, distributed across 12 families and five orders, highlighting the substantial phenotypic and genotypic diversity within this group.[4] This taxonomic diversity suggests that methanogenesis, the metabolic pathway through which methanogens produce methane, is an ancient and widespread trait.[4] The monophyletic nature of modern methanogens indicates that methanogenesis likely evolved only once, with all contemporary methanogens sharing a common ancestor.[4] Recent taxonomic schemes reflect the rich diversity and evolutionary history of methanogens, underscoring their importance in anaerobic microbial ecosystems and their intriguing adaptation to diverse environmental niches.[4]

Each species of Methanobacterium is capable of the syntropic process of methane production, with a majority of the species being hydrogenotrophic.[24] The species differ in their ability to use different substrates for the methane production process. The substrates utilized in the methane production process can be hydrogenotrophic, methylotrophic, or acetoclastic.[24]

Domain: Archaea

Phylum: Euryarchaeota

Class: Methanobacteria

Order: Methanobacteriales

Family: Methanobacteriaceae

Genus: Methanobacterium[25]

Examples of Methanobacterium species

There are many different species of Methanobacterium with officially recognized names.[26] Microbes in the gut, degrade nutrients from feed (polysaccharides, proteins, and fats) into organic molecules which later are turned into methane by Methanobacterium such as Methanobacterium formicicum.[18] Methanobacterium formicicum can be found in the human gut as well as in animals and can cause gastrointestinal and metabolic disorders in both humans and animals.[18]

Methanobacteria oryzae was isolated from

rice field soil in the Philippines, thrive in environments that allow archaea to use hydrogen and acetate as their main energy source.[27] This Methanobacteria as well as other species of Methanobacterium found in rice field soils from around the world are a major source of methane which is a major greenhouse gas.[27]Methanobacterium palustre thrives in marshland areas and was first found in a peat bog.[28] Methanobacterium arcticum was isolated from permafrost sediments in the Russian Arctic.[26] This species of Methanobacterium uses only hydrogen, carbon dioxide, and formate as fuel.[26] Unlike some other Methanobacteria, it does not use acetate
to grow.

Methanobacterium In Human Gut

Methanobacterium are found in the human colon.

bacterial breakdown. [29]

There are two specific groups that have undergone isolation and culture from the human intestines.

amplicon sequencing. [30]

A type of Methanobacterium called M. smithii is specifically found in the human intestines. [31] M. smithii is able to  integrate glycans within the intestines for fixing, which is used for regulating protein expression. [31]An increase of methane concentration in human residue is correlated with BMI. [31]

Methanogens task is to remove hydrogen remains in the gut, based on hydrogen accumulation in the intestines that can reduce the productivity of the microbial activities. [31]Methanogen can, also,be used as probiotics. [31]This is possible since methanogens are capable of using trimethylamine as a substrate for methanogenesis.[31] Trimethylamine is produced in the human intines by intestinal bacteria.[31] An increase of trimethylamine may cause cardiovascular diseases. [31]These methanogens are able to  utilize hydrogen to decrease trimethylamine while it is growing in the intestines. [31]

References

  1. ^ "Supplemental Information 3: Taxon list extracted from taxonomic sources, with corresponding NCBI taxonomy identifiers by which NCBI sequence accessions were filtered". dx.doi.org. Retrieved 2024-02-29.
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  4. ^ a b c d e f g h i j k l m "Methanogen - an overview | ScienceDirect Topics". www.sciencedirect.com. Retrieved 2024-02-29.
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  11. ^ a b c d e f "Midas Field Guide". www.midasfieldguide.org. Retrieved 2024-02-29.
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  13. ISSN 0021-9193. {{cite journal}}: Check date values in: |date= (help
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  14. ^ "Methanobacterium palustre - microbewiki". microbewiki.kenyon.edu. Retrieved 2024-04-02.
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  18. ^ a b c Chellapandi, P (6 December 2018). "Methanobacterium formicicum as a target rumen methanogen for the development of new methane mitigation interventions: A review". Vet Animal Science. 6: 86–94 – via PubMed.
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  25. ^ "WoRMS - World Register of Marine Species - Methanobacterium Kluyver & van Niel, 1936". www.marinespecies.org. Retrieved 2024-03-30.
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  31. ^
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