EGTA

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EGTA (chemical)
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EGTA
Skeletal formula of EGTA
Ball-and-stick model of the EGTA molecule
Names
Preferred IUPAC name
3,12-Bis(carboxymethyl)-6,9-dioxa-3,12-diazatetradecane-1,14-dioic acid
Other names
Triethylene glycol diamine tetraacetic acid
Identifiers
3D model (
JSmol
)
ChEBI
ChEMBL
ChemSpider
ECHA InfoCard
 100.000.592 Edit this at Wikidata
KEGG
UNII
  • InChI=1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24) checkY
    Key: DEFVIWRASFVYLL-UHFFFAOYSA-N checkY
  • InChI=1/C10H13NO2/c1-3-13-10-6-4-9(5-7-10)11-8(2)12/h4-7H,3H2,1-2H3,(H,11,12)
    Key: CPJSUEIXXCENMM-UHFFFAOYAO
  • InChI=1/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
    Key: DEFVIWRASFVYLL-UHFFFAOYAF
  • O=C(O)CN(CC(=O)O)CCOCCOCCN(CC(=O)O)CC(=O)O
Properties
C14H24N2O10
Molar mass 380.350 g·mol−1
Melting point 241 °C (466 °F; 514 K)
Acidity (pKa) 2.0, 2.68, 8.85, 9.43[1]
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)

EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid), also known as egtazic acid (

EDTA. Compared to EDTA, it has a lower affinity for magnesium, making it more selective for calcium ions. It is useful in buffer solutions that resemble the environment in living cells[3]
where calcium ions are usually at least a thousandfold less concentrated than magnesium.

The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91. See Qin et al. for an example of a pKa calculation.[4]

EGTA has also been used experimentally for the treatment of animals with

recombinant fusion proteins are bound to calmodulin
beads and eluted out by adding EGTA.

EGTA is often employed in dentistry and endodontics for the removal of the smear layer.

See also

References

  1. ^ Raaflaub, J. (1956) Methods Biochem. Anal. 3, 301–324.
  2. ^ Pubchem. "EGTA | C14H24N2O10 - PubChem". pubchem.ncbi.nlm.nih.gov. Retrieved 2017-04-24.
  3. ISBN 0-8247-0774-5
    .
  4. PMID 10051660
    .
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