Ferredoxin—nitrite reductase
| ferredoxin—nitrite reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In
enzymology, a ferredoxin—nitrite reductase (EC 1.7.7.1) is an enzyme that catalyzes the chemical reaction
- NH3 + 2 H2O + 6 oxidized ferredoxin nitrite + 6 reduced ferredoxin + 7 H+
The 3
substrates of this enzyme are NH3, H2O, and oxidized ferredoxin, whereas its 3 products are nitrite, reduced ferredoxin, and H+
.
This enzyme belongs to the family of
Iron-sulfur
.
This enzyme can use many different isoforms of ferredoxin. In photosynthesizing tissues, it uses ferredoxin that is reduced by PSI and in the root it uses a form of ferredoxin (FdIII) that has a less negative midpoint potential and can be reduced easily by NADPH.[1]
Structural studies
As of late 2007, 3
structures have been solved for this class of enzymes, with PDB accession codes 1ZJ8, 1ZJ9, and 2AKJ
.
References
- PMID 14684843.
Literature
- Joy KW, Hageman RH (1966). "The purification and properties of nitrite reductase from higher plants, and its dependence on ferredoxin". Biochem. J. 100 (1): 263–73. PMID 4381617.
- Ramirez JM, Del Campo FF, Paneque A, Losada M (1966). "Ferredoxin-nitrite reductase from spinach". Biochim. Biophys. Acta. 118 (1): 58–71. PMID 5954064.
- Zumft WG, Paneque A, Aparicio PJ, Losada M (1969). "Mechanism of nitrate reduction in Chlorella". Biochem. Biophys. Res. Commun. 36 (6): 980–6. PMID 4390523.
