Kynurenine—oxoglutarate transaminase
| kynurenine-oxoglutarate transaminase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
ExPASy NiceZyme view | | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In
enzymology, a kynurenine-oxoglutarate transaminase (EC 2.6.1.7) is an enzyme that catalyzes the chemical reaction
- L-kynurenine + 2-oxoglutarate ⇌ 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Thus, the two
L-glutamate. The former product is an unstable α-oxo acid that quickly undergoes intramolecular cyclization to form kynurenic acid.[1]
This enzyme belongs to the family of
tryptophan metabolism. It employs one cofactor, pyridoxal phosphate
.
KYAT1, AADAT (aka KYAT2), and KYAT3 are examples of enzymes of this class. GOT2 (aka KYAT4) is also believed to catalyze the above reaction.[2]
Structural studies
As of early 2009, 18
structures have been solved for this class of enzymes, with PDB accession codes 1X0M, 1YIY, 1YIZ, 1W7L, 1W7M, 1W7N, 3E2F, 3E2Y, 3E2Z, 2ZJG, 2YGZ, 2Z61, 2R5C, 2R2N, 2R5E, 3B46, 3DC1, and 2QLN
.
References
Further reading
- Bonner DM, Jakoby WB (August 1956). "Kynurenine transaminase from neurospora". The Journal of Biological Chemistry. 221 (2): 689–95. PMID 13357462.
- Mason M (July 1957). "Kynurenine transaminase of rat kidney; a study of coenzyme dissociation". The Journal of Biological Chemistry. 227 (1): 61–8. PMID 13449053.
- Rossi F, Han Q, Li J, Li J, Rizzi M (November 2004). "Crystal structure of human kynurenine aminotransferase I". The Journal of Biological Chemistry. 279 (48): 50214–20. PMID 15364907.
- Chon H, Matsumura H, Koga Y, Takano K, Kanaya S (November 2005). "Crystal structure of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3 at 2.20 A resolution". Proteins. 61 (3): 685–8. S2CID 35477005.
- Han Q, Gao YG, Robinson H, Ding H, Wilson S, Li J (May 2005). "Crystal structures of Aedes aegypti kynurenine aminotransferase". The FEBS Journal. 272 (9): 2198–206. S2CID 38329597.
- Han Q, Robinson H, Cai T, Tagle DA, Li J (February 2009). "Biochemical and structural properties of mouse kynurenine aminotransferase III". Molecular and Cellular Biology. 29 (3): 784–93. PMID 19029248.
- Han Q, Robinson H, Li J (February 2008). "Crystal structure of human kynurenine aminotransferase II". The Journal of Biological Chemistry. 283 (6): 3567–73. PMID 18056995.
- Rossi F, Garavaglia S, Montalbano V, Walsh MA, Rizzi M (February 2008). "Crystal structure of human kynurenine aminotransferase II, a drug target for the treatment of schizophrenia". The Journal of Biological Chemistry. 283 (6): 3559–66. PMID 18056996.
- Han Q, Cai T, Tagle DA, Robinson H, Li J (August 2008). "Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II". Bioscience Reports. 28 (4): 205–15. PMID 18620547.
- Han Q, Gao YG, Robinson H, Li J (February 2008). "Structural insight into the mechanism of substrate specificity of aedes kynurenine aminotransferase". Biochemistry. 47 (6): 1622–30. PMID 18186649.
- Wogulis M, Chew ER, Donohoue PD, Wilson DK (February 2008). "Identification of formyl kynurenine formamidase and kynurenine aminotransferase from Saccharomyces cerevisiae using crystallographic, bioinformatic and biochemical evidence". Biochemistry. 47 (6): 1608–21. PMID 18205391.
