A23187

fermentation of Streptomyces chartreusensis

gram positive bacteria and fungi. It also acts as a divalent cation ionophore, allowing these ions to cross cell membranes, which are usually impermeable to them.^[1] A23187 is most selective for Mn²⁺, somewhat less selective for Ca²⁺ and Mg²⁺, much less selective for Sr²⁺, and even less selective for Ba²⁺.^[2] The ionophore is used in laboratories to increase intracellular Ca²⁺ levels in intact cells. It also uncouples oxidative phosphorylation, the process cells use to synthesize Adenosine triphosphate which they use for energy. In addition, A23187 inhibits mitochondrial ATPase activity. A23187 also induces apoptosis in some cells (e.g. mouse lymphoma cell line, or S49, and Jurkat cells) and prevents it in others (e.g. cells dependent on interleukin 3 that have had the factor withdrawn).^[3]

liposomes loaded with anti-cancer drugs such as Topotecan.^[4]

IVF field, Ca Ionophore can be used in case of low fertilization rate after ICSI procedure, particularly with Globozoospermia (Round Head sperm syndrome), Ca Ionophore will replace absence of sperm acrosome, and plays role in oocyte activation after ICSI. Recommended use is 0.5 microgram/ml twice for 10 min interrupted with fresh media with 30 min incubation, followed with regular injected eggs culture for IVF.^[5]