Adenosine triphosphate

Source: Wikipedia, the free encyclopedia.
Adenosine-5'-triphosphate
Names
IUPAC name
Adenosine 5′-(tetrahydrogen triphosphate)
Systematic IUPAC name
O1-{[(2R,3S,4R,5R)-5-(6-Amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl} tetrahydrogen triphosphate
Identifiers
3D model (
JSmol
)
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard
 100.000.258 Edit this at Wikidata
IUPHAR/BPS
KEGG
UNII
  • InChI=1S/C10H16N5O13P3/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1 checkY
    Key: ZKHQWZAMYRWXGA-KQYNXXCUSA-N checkY
  • Key: ZKHQWZAMYRWXGA-KQYNXXCUBG
SMILES
  • O=P(O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n2cnc1c(ncnc12)N)[C@H](O)[C@@H]3O
  • c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N
Properties
C10H16N5O13P3
Molar mass 507.18 g/mol
Density 1.04 g/cm3 (disodium salt)
Melting point 187 °C (369 °F; 460 K) disodium salt; decomposes
Acidity (pKa) 0.9, 1.4, 3.8, 6.5
UV-vis (λmax) 259 nm[1]
Absorbance
ε259 = 15.4 mM−1 cm−1 [1]
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
checkY verify (what is checkY☒N ?)
A chemical formula
Interactive animation of the structure of ATP

Adenosine triphosphate (ATP) is a

energy transfer.[3]

When consumed in a

coenzyme. An average adult human processes around 50 kilograms (about 100 moles) daily.[4]

From the perspective of biochemistry, ATP is classified as a nucleoside triphosphate, which indicates that it consists of three components: a nitrogenous base (adenine), the sugar ribose, and the triphosphate.

Structure

ATP consists of an adenine attached by the #9-nitrogen atom to the 1′ carbon atom of a sugar (ribose), which in turn is attached at the 5' carbon atom of the sugar to a triphosphate group. In its many reactions related to metabolism, the adenine and sugar groups remain unchanged, but the triphosphate is converted to di- and monophosphate, giving respectively the derivatives ADP and AMP. The three phosphoryl groups are labeled as alpha (α), beta (β), and, for the terminal phosphate, gamma (γ).[5]

In neutral solution, ionized ATP exists mostly as ATP4−, with a small proportion of ATP3−.[6]

Metal cation binding

Polyanionic and featuring a potentially

cation, almost always magnesium, strongly affects the interaction of ATP with various proteins. Due to the strength of the ATP-Mg2+ interaction, ATP exists in the cell mostly as a complex with Mg2+
 bonded to the phosphate oxygen centers.[6][8]

A second magnesium ion is critical for ATP binding in the kinase domain.[9] The presence of Mg2+ regulates kinase activity.[10] It is interesting from an RNA world perspective that ATP can carry a Mg ion which catalyzes RNA polymerization.[citation needed]

Chemical properties

Salts of ATP can be isolated as colorless solids.[11]

The cycles of synthesis and degradation of ATP; 2 and 1 represent input and output of energy, respectively.

ATP is stable in aqueous solutions between

hydrolyses to ADP and phosphate. Living cells maintain the ratio of ATP to ADP at a point ten orders of magnitude from equilibrium, with ATP concentrations fivefold higher than the concentration of ADP.[12][13] In the context of biochemical reactions, the P-O-P bonds are frequently referred to as high-energy bonds.[14]

Reactive aspects

The hydrolysis of ATP into ADP and inorganic phosphate

ATP4-(aq) + H2O(l) = ADP3-(aq) + HPO32-(aq) + H+(aq)

releases 20.5 kilojoules per mole (4.9 kcal/mol) of enthalpy. This may differ under physiological conditions if the reactant and products are not exactly in these ionization states.[15] The values of the free energy released by cleaving either a phosphate (Pi) or a pyrophosphate (PPi) unit from ATP at standard state concentrations of 1 mol/L at pH 7 are:[16]

ATP + H
2O → ADP + Pi   ΔG°' = −30.5 kJ/mol (−7.3 kcal/mol)
ATP + H
2O → AMP + PPi  ΔG°' = −45.6 kJ/mol (−10.9 kcal/mol)

These abbreviated equations at a pH near 7 can be written more explicitly (R = adenosyl):

[RO-P(O)2-O-P(O)2-O-PO3]4− + H
2O → [RO-P(O)2-O-PO3]3− + [HPO4]2− + H+
[RO-P(O)2-O-P(O)2-O-PO3]4− + H
2O → [RO-PO3]2− + [HO3P-O-PO3]3− + H+

At cytoplasmic conditions, where the ADP/ATP ratio is 10 orders of magnitude from equilibrium, the ΔG is around −57 kJ/mol.[12]

Along with pH, the free energy change of ATP hydrolysis is also associated with Mg2+ concentration, from ΔG°' = −35.7 kJ/mol at a Mg2+ concentration of zero, to ΔG°' = −31 kJ/mol at [Mg2+] = 5 mM. Higher concentrations of Mg2+ decrease free energy released in the reaction due to binding of Mg2+ ions to negatively charged oxygen atoms of ATP at pH 7.[17]

This image shows a 360-degree rotation of a single, gas-phase magnesium-ATP chelate with a charge of −2. The anion was optimized at the UB3LYP/6-311++G(d,p) theoretical level and the atomic connectivity modified by the human optimizer to reflect the probable electronic structure.

Production from AMP and ADP

Production, aerobic conditions

A typical intracellular concentration of ATP may be 1–10 μmol per gram of tissue in a variety of eukaryotes.[18] The dephosphorylation of ATP and rephosphorylation of ADP and AMP occur repeatedly in the course of aerobic metabolism.[19]

ATP can be produced by a number of distinct cellular processes; the three main pathways in

beta-oxidation. The overall process of oxidizing glucose to carbon dioxide, the combination of pathways 1 and 2, known as cellular respiration, produces about 30 equivalents of ATP from each molecule of glucose.[20]

ATP production by a non-

mitochondria, which comprise nearly 25% of the volume of a typical cell.[21]

Glycolysis

Main article: Glycolysis

In glycolysis, glucose and glycerol are metabolized to

pyruvate. Glycolysis generates two equivalents of ATP through substrate phosphorylation catalyzed by two enzymes, phosphoglycerate kinase (PGK) and pyruvate kinase. Two equivalents of nicotinamide adenine dinucleotide (NADH) are also produced, which can be oxidized via the electron transport chain and result in the generation of additional ATP by ATP synthase. The pyruvate generated as an end-product of glycolysis is a substrate for the Krebs Cycle.[22]

Glycolysis is viewed as consisting of two phases with five steps each. In phase 1, "the preparatory phase", glucose is converted to 2 d-glyceraldehyde-3-phosphate (g3p). One ATP is invested in Step 1, and another ATP is invested in Step 3. Steps 1 and 3 of glycolysis are referred to as "Priming Steps". In Phase 2, two equivalents of g3p are converted to two pyruvates. In Step 7, two ATP are produced. Also, in Step 10, two further equivalents of ATP are produced. In Steps 7 and 10, ATP is generated from ADP. A net of two ATPs is formed in the glycolysis cycle. The glycolysis pathway is later associated with the Citric Acid Cycle which produces additional equivalents of ATP.[citation needed]

Regulation

In glycolysis,

cyclic AMP, ammonium ions, inorganic phosphate, and fructose-1,6- and -2,6-biphosphate.[22]

Citric acid cycle

Main articles: Citric acid cycle and Oxidative phosphorylation

In the

aerobic process because O2 is used to recycle the NADH and FADH2. In the absence of oxygen, the citric acid cycle ceases.[21]

The generation of ATP by the mitochondrion from cytosolic NADH relies on the

aspartate for transport back across the membrane and into the intermembrane space.[21]

In oxidative phosphorylation, the passage of electrons from NADH and FADH2 through the electron transport chain releases the energy to pump

proton motive force that is the net effect of a pH gradient and an electric potential gradient across the inner mitochondrial membrane. Flow of protons down this potential gradient – that is, from the intermembrane space to the matrix – yields ATP by ATP synthase.[23]
Three ATP are produced per turn.

Although oxygen consumption appears fundamental for the maintenance of the proton motive force, in the event of oxygen shortage (

hypoxia), intracellular acidosis (mediated by enhanced glycolytic rates and ATP hydrolysis), contributes to mitochondrial membrane potential and directly drives ATP synthesis.[24]

Most of the ATP synthesized in the mitochondria will be used for cellular processes in the cytosol; thus it must be exported from its site of synthesis in the mitochondrial matrix. ATP outward movement is favored by the membrane's electrochemical potential because the cytosol has a relatively positive charge compared to the relatively negative matrix. For every ATP transported out, it costs 1 H+. Producing one ATP costs about 3 H+. Therefore, making and exporting one ATP requires 4H+. The inner membrane contains an antiporter, the ADP/ATP translocase, which is an integral membrane protein used to exchange newly synthesized ATP in the matrix for ADP in the intermembrane space.[25]

Regulation

The citric acid cycle is regulated mainly by the availability of key substrates, particularly the ratio of NAD+ to NADH and the concentrations of

Citrate – the ion that gives its name to the cycle – is a feedback inhibitor of citrate synthase and also inhibits PFK, providing a direct link between the regulation of the citric acid cycle and glycolysis.[22]

Beta oxidation

Main article:
Beta-oxidation

In the presence of air and various cofactors and enzymes, fatty acids are converted to

beta-oxidation. Each cycle of beta-oxidation shortens the fatty acid chain by two carbon atoms and produces one equivalent each of acetyl-CoA, NADH, and FADH2. The acetyl-CoA is metabolized by the citric acid cycle to generate ATP, while the NADH and FADH2 are used by oxidative phosphorylation to generate ATP. Dozens of ATP equivalents are generated by the beta-oxidation of a single long acyl chain.[26]

Regulation

In oxidative phosphorylation, the key control point is the reaction catalyzed by cytochrome c oxidase, which is regulated by the availability of its substrate – the reduced form of cytochrome c. The amount of reduced cytochrome c available is directly related to the amounts of other substrates:

which directly implies this equation:

Thus, a high ratio of [NADH] to [NAD+] or a high ratio of [ADP] [Pi] to [ATP] imply a high amount of reduced cytochrome c and a high level of cytochrome c oxidase activity.[22] An additional level of regulation is introduced by the transport rates of ATP and NADH between the mitochondrial matrix and the cytoplasm.[25]

Ketosis

Main article: Ketone bodies

Ketone bodies can be used as fuels, yielding 22 ATP and 2

1,2-propanediol. Though the pathway follows a different series of steps requiring ATP, 1,2-propanediol can be turned into pyruvate.[27]

Production, anaerobic conditions

Fermentation is the metabolism of organic compounds in the absence of air. It involves substrate-level phosphorylation in the absence of a respiratory electron transport chain. The equation for the reaction of glucose to form lactic acid
is:

C
6H
12O
6 + 2 ADP + 2 Pi → 2 CH
3CH(OH)COOH + 2 ATP + 2 H
2O

Anaerobic respiration is respiration in the absence of O
2. Prokaryotes can utilize a variety of electron acceptors. These include nitrate, sulfate, and carbon dioxide.

ATP replenishment by nucleoside diphosphate kinases

ATP can also be synthesized through several so-called "replenishment" reactions catalyzed by the enzyme families of

nucleoside diphosphate kinases (NDKs), which use other nucleoside triphosphates as a high-energy phosphate donor, and the ATP:guanido-phosphotransferase family.[citation needed
]

ATP production during photosynthesis

In plants, ATP is synthesized in the

thylakoid membrane of the chloroplast. The process is called photophosphorylation. The "machinery" is similar to that in mitochondria except that light energy is used to pump protons across a membrane to produce a proton-motive force. ATP synthase then ensues exactly as in oxidative phosphorylation.[28] Some of the ATP produced in the chloroplasts is consumed in the Calvin cycle, which produces triose
sugars.

ATP recycling

The total quantity of ATP in the human body is about 0.1 mol/L.[29] The majority of ATP is recycled from ADP by the aforementioned processes. Thus, at any given time, the total amount of ATP + ADP remains fairly constant.

The energy used by human cells in an adult requires the hydrolysis of 100 to 150 mol/L of ATP daily, which means a human will typically use their body weight worth of ATP over the course of the day.[30] Each equivalent of ATP is recycled 1000–1500 times during a single day (150 / 0.1 = 1500),[29] at approximately 9×1020 molecules/s.[29]

decarboxylase enzyme from the bacterium Staphylococcus epidermidis (PDB: 1G5Q​) with a bound flavin mononucleotide
cofactor

Biochemical functions

Intracellular signaling

ATP is involved in signal transduction by serving as substrate for kinases, enzymes that transfer phosphate groups. Kinases are the most common ATP-binding proteins. They share a small number of common folds.[31] Phosphorylation of a protein by a kinase can activate a cascade such as the mitogen-activated protein kinase cascade.[32]

ATP is also a substrate of

second messenger, cyclic AMP, which is involved in triggering calcium signals by the release of calcium from intracellular stores.[33] This form of signal transduction is particularly important in brain function, although it is involved in the regulation of a multitude of other cellular processes.[34]

DNA and RNA synthesis

ATP is one of four monomers required in the synthesis of

DNA transcription
also consume ATP.

Amino acid activation in protein synthesis

Main article: Amino acid activation

Aminoacyl-tRNA synthetase
enzymes consume ATP in the attachment tRNA to amino acids, forming aminoacyl-tRNA complexes. Aminoacyl transferase binds AMP-amino acid to tRNA. The coupling reaction proceeds in two steps:

  1. aa + ATP ⟶ aa-AMP + PPi
  2. aa-AMP + tRNA ⟶ aa-tRNA + AMP

The amino acid is coupled to the penultimate nucleotide at the 3′-end of the tRNA (the A in the sequence CCA) via an ester bond (roll over in illustration).

ATP binding cassette transporter

Transporting chemicals out of a cell against a gradient is often associated with ATP hydrolysis. Transport is mediated by

ATP binding cassette transporters. The human genome encodes 48 ABC transporters, that are used for exporting drugs, lipids, and other compounds.[36]

Extracellular signalling and neurotransmission

Cells secrete ATP to communicate with other cells in a process called purinergic signalling. ATP serves as a neurotransmitter in many parts of the nervous system, modulates ciliary beating, affects vascular oxygen supply etc. ATP is either secreted directly across the cell membrane through channel proteins[37][38] or is pumped into vesicles[39] which then fuse with the membrane. Cells detect ATP using the purinergic receptor proteins P2X and P2Y.[40] ATP has been shown to be a critically important signalling molecule for microglia - neuron interactions in the adult brain,[41] as well as during brain development.[42] Furthermore, tissue-injury induced ATP-signalling is a major factor in rapid microglial phenotype changes.[43]

Muscle contraction

ATP fuels muscle contractions.[44] Muscle contractions are regulated by signaling pathways, although different muscle types being regulated by specific pathways and stimuli based on their particular function. However, in all muscle types, contraction is performed by the proteins actin and myosin.[45]

ATP is initially bound to myosin. When ATPase hydrolyzes the bound ATP into ADP and inorganic phosphate, myosin is positioned in a way that it can bind to actin. Myosin bound by ADP and Pi forms cross-bridges with actin and the subsequent release of ADP and Pi releases energy as the power stroke. The power stroke causes actin filament to slide past the myosin filament, shortening the muscle and causing a contraction. Another ATP molecule can then bind to myosin, releasing it from actin and allowing this process to repeat.[45][46]

Protein solubility

ATP has recently been proposed to act as a biological hydrotrope[47] and has been shown to affect proteome-wide solubility.[48]

Abiogenic origins

Acetyl phosphate (AcP), a precursor to ATP, can readily be synthesized at modest yields from thioacetate in pH 7 and 20 °C and pH 8 and 50 °C, although acetyl phosphate is less stable in warmer temperatures and alkaline conditions than in cooler and acidic to neutral conditions. It is unable to promote polymerization of ribonucleotides and amino acids and was only capable of phosphorylation of organic compounds. It was shown that it can promote aggregation and stabilization of AMP in the presence of Na+, aggregation of nucleotides could promote polymerization above 75 °C in the absence of Na+. It is possible that polymerization promoted by AcP could occur at mineral surfaces.[49] It was shown that ADP can only be phosphorylated to ATP by AcP and other nucleoside triphosphates were not phosphorylated by AcP. This might explain why all lifeforms use ATP to drive biochemical reactions.[50]

ATP analogues

Biochemistry laboratories often use in vitro studies to explore ATP-dependent molecular processes. ATP analogs are also used in X-ray crystallography to determine a protein structure in complex with ATP, often together with other substrates.[citation needed]

Enzyme inhibitors of ATP-dependent enzymes such as kinases are needed to examine the binding sites and transition states involved in ATP-dependent reactions.[citation needed]

Most useful ATP analogs cannot be hydrolyzed as ATP would be; instead, they trap the enzyme in a structure closely related to the ATP-bound state. Adenosine 5′-(γ-thiotriphosphate) is an extremely common ATP analog in which one of the gamma-phosphate oxygens is replaced by a sulfur atom; this anion is hydrolyzed at a dramatically slower rate than ATP itself and functions as an inhibitor of ATP-dependent processes. In crystallographic studies, hydrolysis transition states are modeled by the bound vanadate ion.

Caution is warranted in interpreting the results of experiments using ATP analogs, since some enzymes can hydrolyze them at appreciable rates at high concentration.[51]

Medical use

ATP is used intravenously for some heart-related conditions.[52]

History

ATP was discovered in 1929 by

Yellapragada Subba Rao of Harvard Medical School,[55]
both teams competing against each other to find an assay for phosphorus.

It was proposed to be the intermediary between energy-yielding and energy-requiring reactions in cells by Fritz Albert Lipmann in 1941.[56]

It was first synthesized in the laboratory by

Alexander Todd in 1948,[57] and he was awarded the Nobel Prize in Chemistry
in 1957 partly for this work.

The 1978 Nobel Prize in Chemistry was awarded to Peter Dennis Mitchell for the discovery of the chemiosmotic mechanism of ATP synthesis.

The 1997 Nobel Prize in Chemistry was divided, one half jointly to

Jens C. Skou "for the first discovery of an ion-transporting enzyme, Na+, K+ -ATPase."[58]

See also

References

  1. ^ a b "Adenosine 5'-triphosphate disodium salt Product Information" (PDF). Sigma. Archived (PDF) from the original on 2019-03-23. Retrieved 2019-03-22.
  2. PMID 31985968
    . Retrieved 13 November 2023.
  3. PMID 6250450
    .
  4. ISBN 9780471754770
    .
  5. PMID 31985968
    , retrieved 2023-09-28
  6. ^
    PMID 11772
    .
  7. PMID 1645933
    .
  8. PMID 3537318
    .
  9. PMID 9730835
    .
  10. PMID 16305747
    .
  11. ISBN 0911910131
    .
  12. ^
    ISBN 978-0-12-518121-1
    .
  13. ISBN 978-0-7167-4684-3
    .
  14. S2CID 4298762
    .
  15. PMID 3528161
    .
  16. ISBN 978-0-7167-8724-2
    .
  17. ISBN 978-1305577206
    .
  18. PMID 1212224
    .
  19. ^ "Adenosine triphosphate". Britannica. 11 November 2023. Retrieved 1 December 2023.
  20. ^
    PMID 14641005
    .
  21. ^
    ISBN 978-0-7167-4366-8
    .
  22. ^
    ISBN 978-0-471-19350-0
    .
  23. S2CID 4275221
    .
  24. PMID 30713504
    .
  25. ^
    PMID 16868313
    .
  26. S2CID 24865576
    .
  27. ^ "Integrated Risk Information System" (PDF). 2013-03-15. Archived (PDF) from the original on 2015-09-24. Retrieved 2019-02-01.
  28. S2CID 1754660
    .
  29. ^
    ISBN 978-0-323-07307-3
    . Retrieved 16 May 2020.
  30. ISBN 978-0-323-07307-3
    . Retrieved 16 May 2020.
  31. PMID 16244704
    .
  32. PMID 16806044
    .
  33. PMID 16934836
    .
  34. PMID 11264454
    .
  35. PMID 7592405
    .
  36. S2CID 34707074. Archived
    (PDF) from the original on 2018-04-21. Retrieved 2018-04-20.
  37. PMID 29739879
    .
  38. PMID 26009770
    .
  39. PMID 21810784
    .
  40. PMID 25530618
    .
  41. PMID 31831638
    .
  42. PMID 36130488
    .
  43. PMID 38926390
    .
  44. PMID 1842855
    .
  45. ^
    PMID 25646377
    .
  46. ^ "38.17: Muscle Contraction and Locomotion - ATP and Muscle Contraction". Biology LibreTexts. 2018-07-16. Retrieved 2024-05-01.
  47. S2CID 24622983
    .
  48. PMID 30858367
    .
  49. PMID 29502283
    .
  50. ^ "Ancient chemistry may explain why living things use ATP as the universal energy currency: An early step in metabolic evolution set the stage for emergence of ATP as the universal energy carrier". ScienceDaily. Retrieved 2023-08-27.
  51. PMID 8519760
    .
  52. PMID 22057692
    .
  53. S2CID 20328411
    .
  54. PMID 12161449. Archived
    from the original on 2017-08-08. Retrieved 2017-10-24.
  55. S2CID 87425890
    .
  56. ISSN 0196-7398
    .
  57. ^ "History: ATP first discovered in 1929". The Nobel Prize in Chemistry 1997. Nobel Foundation. Archived from the original on 2010-01-23. Retrieved 2010-05-26.
  58. ^ "The Nobel Prize in Chemistry 1997". Nobel Prize. Archived from the original on 24 October 2017. Retrieved 21 January 2018.
Wikimedia Commons has media related to Adenosine triphosphate.
Retrieved from "https://en.wikipedia.org/w/index.php?title=Adenosine_triphosphate&oldid=1284914921"