Luxol fast blue stain

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Micrograph of the pons using a hematoxylin & eosin-luxol fast blue stain.
Coronal section of a mouse brain stained with Hematoxylin & LFB

Luxol fast blue stain, abbreviated LFB stain or simply LFB, is a commonly used

demyelination in the central nervous system (CNS), but cannot discern myelination in the peripheral nervous system.[2]

Procedure

Luxol fast blue is a

Under the stain,

nerve cells appear purple. Tissues sections are treated over an extended period of time (usually overnight) and then differentiated with a lithium carbonate solution.[4]

Combination methods

The combination of LFB with a variety of common staining methods provides the most useful and reliable method for the demonstration of pathological processes in the CNS.

periodic acid-Schiff, Oil Red O, phosphotungstic acid, and Holmes silver nitrate method.[2]

See also

References