Pribnow box
The Pribnow box (also known as the Pribnow-Schaller box) is a sequence of TATAAT of six
—that is, it shows the most frequently occurring base at each position in many promoters analyzed; individual promoters often vary from the consensus at one or more positions. It is also commonly called the -10 sequence or element, because it is centered roughly ten base pairs upstream from the site of initiation of transcription.The Pribnow box has a function similar to the TATA box that occurs in promoters in eukaryotes and archaea: it is recognized and bound by a subunit of RNA polymerase during initiation of transcription.[3] This region of the DNA is also the first place where base pairs separate during prokaryotic transcription to allow access to the template strand. The AT-richness is important to allow this separation, since adenine and thymine are easier to break apart (not only due to fewer hydrogen bonds, but also due to weaker base stacking effects).[4]
It is named after David Pribnow and Heinz Schaller.[citation needed][1][2]
Probability of occurrence of each nucleotide in E. coli
| T | A | T | A | A | T |
|---|---|---|---|---|---|
| 82% | 89% | 52% | 59% | 49% | 89% |
In fiction
The term "Pribnow box" is used in episode 13 of Neon Genesis Evangelion, in reference to the chamber holding simulation Evangelions for testing purposes.
See also
References
- ^ a b
Pribnow, David (March 1975). "Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter". PMID 1093168.
- ^ a b
Schaller, Heinz; Gray, Christopher; Herrman, Karin (February 1975). "Nucleotide sequence of an RNA polymerase binding site from the DNA of Bacteriophage fd". PMID 1054851.
- PMID 22136875.
- ^
Yakovchuk, Peter; Protozanova, Ekaterina; Frank-Kamenetskii, Maxim D. (January 2006). "Base-stacking and base-pairing contributions into thermal stability of the DNA double helix". PMID 16449200.
- PMID 3550697.
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