Tamir Gonen
Tamir Gonen | |
---|---|
PhD ) | |
Awards |
|
Scientific career | |
Fields | Membrane protein Structural biology cryoEM MicroED |
Institutions | Howard Hughes Medical Institute University of California, Los Angeles Janelia Research Campus University of Washington Harvard Medical School |
Thesis | Novel protein-protein interactions in the lens: a solution to the Mp20 enigma |
Doctoral advisor | Edward N. Baker Joerg Kistler |
Other academic advisors | Thomas Walz |
Website | https://cryoem.ucla.edu/ |
Tamir Gonen (born 1975) is an American structural biochemist and membrane biophysicist best known for his contributions to
Education
Gonen attended the University of Auckland in New Zealand and graduated with a Bachelor of Science double major in Inorganic Chemistry and Biological Sciences, followed by First Class Honors in Biological Sciences in 1998. He then obtained a Doctor of Philosophy in Biological Science in 2002 from the University of Auckland for research with by Edward N. Baker and Joerg Kistler.[1] Postdoctoral education was conducted at Harvard Medical School at the laboratory of Thomas Walz.
Research
Gonen's current research focuses on the structures and functions of medically important membrane proteins that are involved in homeostasis and method development in cryoEM, namely microcrystal electron diffraction (microED). He published the first atomic resolution structure determined by cryoEM detailing the structure of aquaporin-0 at 1.9Å resolution.[2]
Development of microcrystal electron diffraction
The Gonen laboratory spearheaded the use of electron diffraction for the determination of protein structure from 3D nano crystals in a frozen hydrated state.[3][4][5] The method termed microED was established in 2013 with a proof of principle paper published in eLife.[6] In 2014 continuous rotation MicroED was established and demonstrated.[7] In 2015 the first novel structure was determined by MicroED for the protein alpha-synuclein at 1.4Å resolution[8] in collaboration with David Eisenberg and in 2016 microED yielded 1Å resolution data from protein nanocrystals where the phase could be solved ab initio.[9] MicroED has been used for drug discovery,[10] determination of membrane proteins such as ion channels[11] materials[12] and small organic molecules studied in a frozen hydrated state[13][14] and extended to sub atomic resolution better than 0.8Å.[15]
Career
- Postdoctoral fellow, Harvard medical School (2002–2005)
- Assistant professor, University of Washington, Seattle (2005–2010)
- Early career scientist, Howard Hughes Medical Institute (2009–2011)
- Associate professor with tenure, University of Washington, Seattle (2011)
- Group leader, Howard Hughes Medical Institute Janelia Research Campus (2011–2017)
- Professor of biological chemistry and physiology, University of California Los Angeles, David Geffen School of Medicine (2017–Present)
- Investigator, Howard Hughes Medical Institute (2017–Present)
Honors
- First Class Honors in Biological Sciences (University of Auckland, 1998)
- Career Development Award, American Diabetes Association (2009)
- Member, Royal Society of New Zealand (2014)
- Chair, Biophysical Society CryoEM subgroup (2018)
- A.L. Patterson Award from the American Crystallographic Association (2023)
Memberships
2014 Royal Society of New Zealand
References
- hdl:2292/1094.
- PMID 16319884.
- S2CID 38786632.
- ^ Curry, Stephen (2013-11-19). "The Goldilocks Protocol: electrons sent in to put microcrystals to work for structural biology | Stephen Curry". the Guardian. Retrieved 2018-07-31.
- S2CID 29710840.
- PMID 24252878.
- PMID 25086503.
- PMID 26352473.
- PMID 27647903.
- bioRxiv 10.1101/241182.
- PMID 30167468.
- PMID 29072840.
- PMID 29335561.
- PMID 30555912.
- PMID 29439243.