Ent-kaurene synthase
| ent-kaurene synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme ent-kaurene synthase (EC 4.2.3.19) catalyzes the chemical reaction
- ent-copalyl diphosphate ent-kaurene + diphosphate
This enzyme belongs to the family of
diterpenoid biosynthesis
.
In Stevia
In transcription of steviol glycoside synthesis genes including ent-kaurene synthase.[1][2] This is believed to reduce steviol content in the final plant product.[1][2]
References
- ^ ISSN 0175-7598.
- ^ .
- Fall RR, West CA (1971). "Purification and properties of kaurene synthetase from Fusarium moniliforme". J. Biol. Chem. 246 (22): 6913–28. PMID 4331199.
- Yamaguchi S, Saito T, Abe H, Yamane H, Murofushi N, Kamiya Y (1996). "Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.)". Plant J. 10 (2): 203–13. PMID 8771778.
- Kawaide H, Imai R, Sassa T, Kamiya Y (1997). "Ent-kaurene synthase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis". J. Biol. Chem. 272 (35): 21706–12. PMID 9268298.
- W, Sassa T; Kawaide, H; Ishizaki, A; Shinoda, S; Otsuka, M; Mitsuhashi, W; Sassa, T (2000). "Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase". Biosci. Biotechnol. Biochem. 64 (3): 660–4. PMID 10803977.
