Methylotroph
Methylotrophs are a diverse group of
Metabolism
The key intermediate in methylotrophic metabolism is formaldehyde, which can be diverted to either assimilatory or dissimilatory pathways.[2] Methylotrophs produce formaldehyde through oxidation of methanol and/or methane. Methane oxidation requires the enzyme methane monooxygenase (MMO).[3][4] Methylotrophs with this enzyme are given the name methanotrophs. The oxidation of methane (or methanol) can be assimilatory or dissimilatory in nature (see figure). If dissimilatory, the formaldehyde intermediate is oxidized completely into to produce reductant and energy.[5][6] If assimilatory, the formaldehyde intermediate is used to synthesize a 3-Carbon () compound for the production of biomass.[2][7] Many methylotrophs use multi-carbon compounds for anabolism, thus limiting their use of formaldehyde to dissimilatory processes, however methanotrophs are generally limited to only metabolism.[2][5]
Single Carbon Compounds | Chemical Formula | Multi-Carbon Compounds | Chemical Formula |
---|---|---|---|
Carbon monoxide | Dimethyl ether | ||
Formaldehyde | Dimethylamine | ||
Formamide | Dimethyl sulfide | ||
Formic acid | Tetramethylammonium | ||
Methane | Trimethylamine | ||
Methanol | Trimethylamine N-oxide | ||
Methylamine | Trimethylsuphonium | ||
Methyl halide |
Catabolism
Methylotrophs use the electron transport chain to conserve energy produced from the oxidation of compounds. An additional activation step is required in methanotrophic metabolism to allow degradation of chemically-stable methane. This oxidation to methanol is catalyzed by MMO, which incorporates one oxygen atom from into methane and reduces the other oxygen atom to water, requiring two equivalents of reducing power.[4][5] Methanol is then oxidized to formaldehyde through the action of methanol dehydrogenase (MDH) in bacteria,[12] or a non-specific alcohol oxidase in yeast.[13] Electrons from methanol oxidation are passed to a membrane-associated quinone of the electron transport chain to produce .[14]
In dissimilatory processes, formaldehyde is completely oxidized to and excreted. Formaldehyde is oxidized to formate via the action of Formaldehyde dehydrogenase (FALDH), which provides electrons directly to a membrane associated quinone of the electron transport chain, usually cytochrome b or c.[2][5] In the case of associated dehydrogenases, is produced.[7]
Finally, formate is oxidized to by cytoplasmic or membrane-bound Formate dehydrogenase (FDH), producing [15] and .
Anabolism
The main metabolic challenge for methylotrophs is the assimilation of single carbon units into biomass. Through de novo synthesis, methylotrophs must form carbon-carbon bonds between 1-Carbon () molecules. This is an energy intensive process, which facultative methylotrophs avoid by using a range of larger organic compounds.[16] However, obligate methylotrophs must assimilate molecules.[2][5] There are four distinct assimilation pathways with the common theme of generating one molecule.[2] Bacteria use three of these pathways[7][11] while Fungi use one.[17] All four pathways incorporate 3 molecules into multi-carbon intermediates, then cleave one intermediate into a new molecule. The remaining intermediates are rearranged to regenerate the original multi-carbon intermediates.
Bacteria
Each species of methylotrophic bacteria has a single dominant assimilation pathway.[5] The three characterized pathways for carbon assimilation are the ribulose monophosphate (RuMP) and serine pathways of formaldehyde assimilation as well as the ribulose bisphosphate (RuBP) pathway of CO2 assimilation.[2][7][11][18]
Ribulose bisphosphate (RuBP) cycle
Unlike the other assimilatory pathways, bacteria using the RuBP pathway derive all of their organic carbon from assimilation.[5][19] This pathway was first elucidated in photosynthetic autotrophs and is better known as the Calvin Cycle.[19][20] Shortly thereafter, methylotrophic bacteria who could grow on reduced compounds were found using this pathway.[21]
First, 3 molecules of ribulose 5-phosphate are phosphorylated to
Ribulose monophosphate (RuMP) cycle
A new pathway was suspected when RuBisCO was not found in the methanotroph Methylmonas methanica.[22] Through radio-labelling experiments, it was shown that M. methanica used the Ribulose monophate (RuMP) pathway. This has led researchers to propose that the RuMP cycle may have preceded the RuBP cycle.[5]
Like the RuBP cycle, this cycle begins with 3 molecules of ribulose-5-phosphate. However, instead of phosphorylating ribulose-5-phosphate, 3 molecules of formaldehyde form a C-C bond through an aldol condensation, producing 3 molecules of 3-hexulose 6-phosphate (hexulose phosphate). One of these molecules of hexulose phosphate is converted into GAP and either
Serine cycle
Unlike the other assimilatory pathways, the serine cycle uses carboxylic acids and amino acids as intermediates instead of carbohydrates.
Yeasts
Methylotrophic yeast metabolism differs from bacteria primarily on the basis of the enzymes used and the carbon assimilation pathway. Unlike bacteria which use bacterial MDH, methylotrophic yeasts oxidize methanol in their peroxisomes with a non-specific alcohol oxidase. This produces formaldehyde as well as hydrogen peroxide.[24][25] Compartmentalization of this reaction in peroxisomes likely sequesters the hydrogen peroxide produced. Catalase is produced in the peroxisomes to deal with this harmful by-product.[17][24]
Dihydroxyacteone (DHA) cycle
The dihydroxyacetone (DHA) pathway, also known as the xylulose monophosphate (XuMP) pathway, is found exclusively in yeast.[24][26] This pathway assimilates three molecules of formaldehyde into 1 molecule of DHAP using 3 molecules of xylulose 5-phosphate as the key intermediate.
DHA synthase acts as a transferase (transketolase) to transfer part of xylulose 5-phosphate to DHA. Then these 3 molecules of DHA are phosphorylated to DHAP by triokinase. Like the other cycles, 3 molecules are produced with 1 molecule being directed for use as cell material. The other 2 molecules are used to regenerate xylulose 5-phosphate.[27]
Environmental Implications
As key players in the carbon cycle, methylotrophs work to reduce global warming primarily through the uptake of methane and other greenhouse gases. In aqueous environments, methanogenic archaea produce 40-50% of the world's methane. Symbiosis between methanogens and methanotrophic bacteria greatly decreases the amount of methane released into the atmosphere.[28]
This symbiosis is also important in the marine environment. Marine bacteria are very important to
Because these compounds are volatile and impact the climate and atmosphere, research on the interaction of these bacteria with these one-carbon compounds can also help understanding of air-sea fluxes of these compounds, which impact climate predictions.[31][29] For example, it is uncertain whether the ocean acts as a net source or sink of atmospheric methanol, but a diverse set of methylotrophs use methanol as their main energy source. In some regions, methylotrophs have been found to be a net sink of methanol,[32] while in others a product of methylotroph activity, methylamine, has been found to be emitted from the ocean and form aerosols.[29] The net direction of these fluxes depends on the utilization by methylotrophs.
Studies have found that methylotrophic capacity varies with the productivity of a system, so the impacts of methylotrophy are likely seasonal. Because some of the one-carbon compounds used by methylotrophs, such as
Impacts of methylotrophs were also found in
The use of methylotrophs in the agricultural sector is another way in which they can potentially impact the environment. Traditional
References
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