Serial block-face scanning electron microscopy
Serial block-face scanning electron microscopy is a method to generate high resolution three-dimensional images from small samples. The technique was developed for brain tissue, but it is widely applicable for any biological samples.
Applications
One of the first applications of serial block-face scanning electron microscopy was to analyze the connectivity of
Many different samples can be prepared for serial block-face scanning electron microscopy and the ultramicrotome is able to cut many materials, therefore this technique has wider applicability. It is starting to find applications in many other areas ranging from cell and developmental biology to materials science.[8]
Advantages and disadvantages
A disadvantage encountered with the SBEM method is that the thickness of the slice which can be removed with the ultra-microtome is limited (~25 nm), thus the resolution in the depth direction is limited. An advantage of the SBEM technique is that the specimen is stationary what improves the alignment in the stacks of images. Another advantage of the SBEM technique is the ability to acquire large data sets with a high level of detail. Because cutting by the ultra-microtome is extremely fast (comparing to the milling process in FIB-SEM), it can expose a wide area of the material (x and y directions) every sectioning. Additionally, by fast cutting, we can acquire many images in z-direction in a short period of time.[1]
See also
References
- ^ PMID 15514700.
- PMID 27501303.
- PMID 26235643.
- ^ "3View System for Image Capture of 3D Ultrastructures | Gatan, Inc".
- ^ "Teneo VolumeScope SEM for Life Sciences". www.fei.com. Mark Anderson. 2017-10-02. Retrieved 2017-10-09.
{{cite web}}
: CS1 maint: others (link) - ^ "Katana microtome".
- ^ "Challenge << EyeWire". Archived from the original on April 14, 2012. Retrieved March 27, 2012.
- PMID 29977493.