Yeast artificial chromosome

Source: Wikipedia, the free encyclopedia.
This article is about Chromosomes derived from yeast DNA. For Yeast created from synthetic DNA, see
Synthetic DNA
.

Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast,

chromosome walking. This is the process that was initially used for the Human Genome Project, however due to stability issues, YACs were abandoned for the use of bacterial artificial chromosome [2]

The bakers' yeast S. cerevisiae is one of the most important experimental organisms for studying eukaryotic molecular genetics.[1]

Beginning with the initial research of the Rankin et al., Strul et al., and Hsaio et al., the inherently fragile chromosome was stabilized by discovering the necessary autonomously replicating sequence (ARS);[2] a refined YAC utilizing this data was described in 1983 by Murray et al.[3]

The primary components of a YAC are the ARS,

telomeres [4] from S. cerevisiae. Additionally, selectable marker genes, such as antibiotic resistance and a visible marker, are utilized to select transformed yeast cells. Without these sequences, the chromosome will not be stable during extracellular replication, and would not be distinguishable from colonies without the vector.[4]

This is a photo of two copies of the Washington University Human Genome YAC Library. Each of the stacks is approximately 12 microtiter plates. Each plate has 96 wells, each with different yeast clones.

Construction

A YAC is built using an initial circular DNA plasmid, which is typically cut into a linear DNA molecule using restriction enzymes; DNA ligase is then used to ligate a DNA sequence or gene of interest into the linearized DNA, forming a single large, circular piece of DNA.[3] [5] The basic generation of linear yeast artificial chromosomes can be broken down into 6 main steps:

  1. Ligation of selectable marker into plasmid vector: this allows for the differential selection of colonies with, or without the marker gene. An
    antibiotic resistance gene allows the YAC vector to be amplified and selected for in E. coli by rescuing the ability of mutant E. coli to synthesize leucine in the presence of the necessary components within the growth medium. TRP1 and URA3 genes are other selectable markers. The YAC vector cloning site for foreign DNA is located within the SUP4 gene. This gene compensates for a mutation in the yeast host cell that causes the accumulation of red pigment. The host cells are normally red, and those transformed with YAC only, will form colorless colonies. Cloning of a foreign DNA fragment into the YAC causes insertional inactivation of the gene, restoring the red color. Therefore, the colonies that contain the foreign DNA fragment are red.[5]
  2. Ligation of necessary centromeric sequences for mitotic stability[6]
  3. Ligation of Autonomously Replicating Sequences (ARS) providing an origin of replication to undergo mitotic replication. This allows the plasmid to replicate extrachromosomally, but renders the plasmid highly mitotically unstable, and easily lost without the centromeric sequences.[4][7]
  4. Ligation of artificial telomeric sequences to convert circular plasmid into a linear piece of DNA [8]
  5. Insertion of DNA sequence to be amplified (up to 1000kb)
  6. Transformation yeast colony[9]

Full chromosome III

Chromosome III is the third smallest chromosome in S. cerevisiae; its size was estimated from pulsed-field gel electro- phoresis studies to be 300–360 kb[10]

This chromosome has been the subject of intensive study, not least because it contains the three genetic loci involved in mating-type control: MAT, HML and HMR.[11] In March 2014,

S. cerevisiae 16 yeast chromosomes, the chromosome III, that he named synIII.[12][13]
The procedure involved replacing the genes in the original chromosome with synthetic versions and the finished synthesized chromosome was then integrated into a yeast cell. It required designing and creating 273,871 base pairs of DNA - fewer than the 316,667 pairs in the original chromosome.

Uses in biotechnology

Yeast expression vectors, such as YACs,

posttranslational modification. By being able to insert large fragments of DNA, YACs can be utilized to clone and assemble the entire genomes of an organism.[14]
With the insertion of a YAC into yeast cells, they can be propagated as linear artificial chromosomes, cloning the inserted regions of DNA in the process. With this completed, two processes can be used to obtain a sequenced genome, or region of interest:

  1. Physical Mapping [6]
  2. Chromosome Walking[15]

This is significant in that it allows for the detailed mapping of specific regions of the genome. Whole human chromosomes have been examined, such as the X chromosome,[16] generating the location of genetic markers for numerous genetic disorders and traits.[17]

Schematic of the pBR322 plasmid generated in the Boyer Lab at UC San Francisco by Bolivar and Rodriguez in 1972. It is one of the first and most widely utilized vectors, and the foundation for the YACs created by Murray and Szostak in 1983 The plasmid contains ampicillin and tetracycline resistance genes, and a suite of restriction enzyme target sites for inserting DNA fragments.

The Human Genome Project

In the United States, the Human Genome Project first took clear form in February of 1988, with the release of the National Research Council (NRC) report Mapping and Sequencing the Human Genome.

Chimerism may be due to either co-ligation of multiple genomic segments into a single YAC, or recombination of two or more YACs transformed in the same host Yeast cell.[19] The incidence of chimerism may be as high as 50%.[20] Other artifacts are deletion of segments from a cloned region, and rearrangement of genomic segments (such as inversion). In all these cases, the sequence as determined from the YAC clone is different from the original, natural sequence, leading to inconsistent results and errors in interpretation if the clone's information is relied upon. Due to these issues, the Human Genome Project ultimately abandoned the use of YACs and switched to bacterial artificial chromosomes, where the incidence of these artifacts is very low. In addition to stability issues, specifically the relatively frequent occurrence of chimeric events, YACs proved to be inefficient when generating the minimum tiling path covering the entire human genome. Generating the clone libraries is time consuming. Also, due to the nature of the reliance on sequence tagged sites (STS) as a reference point when selecting appropriate clones, there are large gaps that need further generation of libraries to span. It is this additional hindrance that drove the project to utilize BACs instead.[21] This is due to two factors:[22]

  1. BACs are much quicker to generate, and when generating redundant libraries of clones, this is essential
  2. BACs allow more dense coverage with STSs, resulting in more complete and efficient minimum tiling paths generated in silico.

However, it is possible to utilize both approaches, as was demonstrated when the genome of the nematode, C. elegans. There majority of the genome was tiled with BACs, and the gaps filled in with YACs.[21]

See also

References

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  2. PMID 386351
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  3. ^
    S2CID 4337825
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  4. ^
    PMID 322128
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  5. ^ Strachan T. (2011). Human molecular genetics / Tom Strachan and Andrew Read, 4th ed.
  6. S2CID 4348814
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  7. PMID 375221
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  8. PMID 6765606
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  9. PMID 3033825
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  10. S2CID 4271784
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  11. S2CID 4271784.{{cite journal}}: CS1 maint: multiple names: authors list (link
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  12. ^ Shukman, David (27 March 2014). "Scientists hail synthetic chromosome advance". BBC News. Retrieved 2014-03-28.
  13. PMID 24674868
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  14. ^ Burke, D., Carle, G. & Olson, M. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236, 806–812 (1987).
  15. PMID 1427839
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  16. PMID 15772651
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  17. S2CID 12997875
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  18. PMID 8506271
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  19. PMID 7713499
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  20. PMID 2000377
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  21. ^ a b Rowen, L., Mahairas, G. & Hood, L. Sequencing the Human Genome. Science (1997).
  22. PMID 11237014
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External links
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