LB buffer

LB buffer, also known as lithium borate buffer, is a

Lithium Borate buffer has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-50 V/cm as compared to 5-10 V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time.^[1] Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected with lithium boric acid gels. ^[2][3]

SB buffer containing sodium borate is similar to lithium borate and has nearly all of its advantages at a somewhat lower cost, but the lithium buffer permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions and has a better resolution for fragments above 4kb.

See also

• Borate
• Lithium
• SB buffer
• TBE buffer

External links

• Sodium borate as an electrophoresis buffer

References

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