Metaphase
Metaphase (from
genetic information, align in the equator of the cell between the spindle poles at the metaphase plate, before being separated into each of the two daughter nuclei. This alignment marks the beginning of metaphase.[2] Metaphase accounts for approximately 4% of the cell cycle's duration.[citation needed
]
In metaphase, microtubules from both duplicated centrosomes on opposite poles of the cell have completed attachment to kinetochores on condensed chromosomes. The centromeres of the chromosomes convene themselves on the metaphase plate, an imaginary line that is equidistant from the two spindle poles.[3] This even alignment is due to the counterbalance of the pulling powers generated by the opposing kinetochore microtubules,[4] analogous to a tug-of-war between two people of equal strength, ending with the destruction of B cyclin.[5]
In order to prevent deleterious
proteosome
. As long as securin and cyclin B remain active, separase remains inactive, preventing premature progression to anaphase.
Metaphase in cytogenetics and cancer studies
The analysis of metaphase
Quinacrine, produces a pattern of in total up to several hundred bands. Normal metaphase spreads are used in methods like FISH and as a hybridization matrix for comparative genomic hybridization
(CGH) experiments.
bcr-abl in chronic myelogenous leukemia
.
References
- ^ "Chromosome condensation through mitosis". ScienceDaily. Retrieved 12 June 2007.
- ISBN 9780393680393.
- ^ "Metaphase plate". Biology Dictionary. Biology Online. Retrieved 9 December 2012.
- ^ "Metaphase". Nature Education. Retrieved 9 December 2012.
- ^ "The Cell Cycle". Kimball's Biology Pages. Archived from the original on 19 November 2012. Retrieved 9 December 2012.
External links
Media related to Metaphase at Wikimedia Commons
