Oxidase
In biochemistry, an oxidase is an oxidoreductase (any enzyme that catalyzes a redox reaction) that uses dioxygen (O2) as the electron acceptor. In reactions involving donation of a hydrogen atom, oxygen is reduced to water (H2O) or hydrogen peroxide (H2O2). Some oxidation reactions, such as those involving monoamine oxidase or xanthine oxidase, typically do not involve free molecular oxygen.[1][2]
The oxidases are a subclass of the oxidoreductases. The use of dioxygen is the only unifying feature; in the EC classification, these enzymes are scattered in many categories.
Examples
An important example is EC 7.1.1.9
- EC 1.1.3.4 Glucose oxidase
- EC 1.4.3.4 Monoamine oxidase
- EC 1.14.-.- Cytochrome P450 oxidase
- EC 1.6.3.1 NADPH oxidase
- EC 1.17.3.2 Xanthine oxidase
- EC 1.1.3.8 L-gulonolactone oxidase
- EC 1.10.3.2 Laccase
- EC 1.4.3.13 Lysyl oxidase
- EC 1.10.3.2 Polyphenol oxidase
- Sulfhydryl oxidase. This enzyme oxidises thiolgroups.
Oxidase test
In
The test is used to determine whether a bacterium is an aerobe or anaerobe. However a bacterium that is Oxidase negative is not necessarily anaerobic, instead showing the bacterium does not possess cytochrome c oxidase.
References
- ISBN 978-0471205036.
- ISBN 978-0198502296.
External links
- Catalase & Oxidase tests video
- Oxidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)