Thermus thermophilus

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Thermus thermophilus
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Deinococcota
Class: Deinococci
Order: Thermales
Family: Thermaceae
Genus: Thermus
Species:
T. thermophilus
Binomial name
Thermus thermophilus
(ex Oshima and Imahori 1974) Manaia et al. 1995

Thermus thermophilus is a

composting.[2]
T. thermophilus is classified into several strains, of which HB8 and HB27 are the most commonly used in laboratory environments. Genome analyses of these strains were independently completed in 2004.
transformation known to date. [4]

Cell structure

Thermus thermophilus is a

Gram-positive bacterium, but the amount, the degree of the cross-linkage and length of the glycan chain gives this bacterium its Gram-negative properties.[5]

Survival mechanisms

Thermus thermophilus was originally found within a

thermal vent in Japan. These bacteria can be found in a variety of geothermal environments. These Thermophiles require a more stringent DNA repair system, as DNA becomes unstable at high temperatures. The GC-content of this bacterium is about 69%, this contributes to the thermostability of this bacterium's genome.[6]

Strains

The two most widely used strains in laboratory settings are HB27 and HB8. The strain HB27 is capable of living in an

megaplasmid, known as pTT27 (0.23Mb long).[7] The chromosome of HB27 contains 1,968 protein coding genes, with 20% of these genes having no known function. While the megaplasmid contains 230 protein coding genes, about 39% of these genes have no known function.[8]

The strain HB8 is also an

polyploid organism, with a chromosome and megaplasmid copy number of about four to five.[7]

Applications

This organism has been advantageous for industrial biotechnological fields as it is an excellent source of enzymes, more specifically thermozymes. One of these enzymes being the Tth DNA polymerase (rTth to emphasize it being recombinant).

DNA polymerase I, thermostable
Identifiers
OrganismThermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
SymbolpolA
UniProt
P52028
Search for
StructuresSwiss-model
DomainsInterPro

rTth DNA polymerase is a recombinant thermostable DNA polymerase derived from Thermus thermophilus HB8, with optimal activity at 70-80 °C, used in some PCR applications. The enzyme possesses efficient reverse transcriptase activity in the presence of manganese.[9] This enzyme is beneficial for amplification of GC-rich targets and for crude samples. It can be used in applications of PCR, RT-PCR and also primer extension.[10] This polymerase has been shown to be resistant to DNA polymerase inhibitors present in clinical samples, it also has the capacity to detect RNA in the presence of inhibitors. Under the presence of inhibitors, it was shown to detect this RNA at a comparable level with its capacity to detect DNA.[9]

See also

References

  1. doi:10.1099/00207713-24-1-102
    .
  2. ^ Beffa T, Blanc M, Lyon PF, Vogt G, Marchiani M, Fischer JL, Aragno M (May 1996). "Isolation of Thermus strains from hot composts (60 to 80 °C)". Applied and Environmental Microbiology. 62 (5): 1723–7.
    PMID 8633870
    .
  3. ^ Henne A, Brüggemann H, Raasch C, Wiezer A, Hartsch T, Liesegang H, et al. (May 2004). "The genome sequence of the extreme thermophile Thermus thermophilus". Nature Biotechnology. 22 (5): 547–53.
    S2CID 25469576
    .
  4. PMID 3957870
    .
  5. PMID 7665471
    .
  6. PMID 25729055
    .
  7. ^
    PMID 20729360
    .
  8. PMID 25605305
    .
  9. ^
    PMID 29293599
    .
  10. ^ "rTth DNA Polymerase - TOYOBO USA". www.toyobousa.com. Retrieved 2021-05-07.

External links

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