C8orf82

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Chromosome 8 open reading frame 82 is a protein encoded in humans by the C8orf82 gene.

Gene

C8orf82 is a gene located on the minus strand of chromosome 8 in the species Homo sapiens at locus 8q24. The gene is 3,400 bases long and contains 3 exons.[1]

mRNA

The mRNA produced by the C8orf82 gene is 1,979 base pairs long with coding sequence that starts at base 159 and ends at base 809.[2] According to NCBI Gene there are no alternative transcripts or isoforms.[1] AceView lists several alternative transcripts and isoforms, the existence of which is uncertain.[3]

Homology

C8orf82 is conserved among invertebrates, fish, reptiles, mammals, and protists. C8orf82 was not found to be conserved among bacteria, archaea, plants, fungi, or trichoplax. The domain of unknown function DUF4505 seems to be well conserved among most orthologs.[1]

Orthologs

Below is a table of orthologs obtained using Blast.

Common name Genus & species Date of divergence from humans (MYA) Accession number Sequence length (AA) Sequence identity to humans Sequence similarity to humans
Chimpanzee
Pan troglodytes
 6.6 NP_001233407 216 98% 98%
Little brown bat Myotis lucifugus 97.5 XP_006107626 157 91% 92%
Wild boar Sus scrofa 97.5 XP_013851838 269 53% 56%
Peregrine falcon Falco peregrinus 320.5 XP_013149891 205 70% 76%
Burmese python Python bivittatus 320.5 XP_007436434 201 64% 75%
Schlegel's Japanese gecko
Gekko japonicus
 320.5 XP_015273778 214 65% 74%
Crested ibis Nipponia nippon 320.5 XP_009466919 168 66% 72%
Rock dove Columba livia 320.5 XP_013226589 159 59% 67%
Kiwi bird
Apteryx australis mantelli
 320.5 XP_013800585 318 55% 64%
Green turtle
Chelonia mydas
 320.5 XP_007063392 188 52% 64%
Blind cave fish Astyanax mexicanus 429.6 XP_007255700 217 63% 75%
Black cod Notothenia coriiceps 429.6 XP_010788219 237 61% 71%
Sheepshead minnow Cyprinodon variegatus 429.6 XP_015227424 166 57% 65%
Sea urchin Strongylocentrotus purpuratus 747.8 XP_784244 265 54% 72%
Swallowtail butterfly Papilio polytes 847 XP_013133745 166 52 72
Honey bee Apis mellifera 847 XP_393829.2 220 50% 64%
Mite Metaseiulus occidentalis 847 XP_003744318 211 42% 60%
Sponge Amphimedon queenslandica 936 XP_003384611 215 47% 62%
Protozoa Tetrahymena thermophila 1724.7 XP_001015777.2 208 30% 46%
Protist
Thecamonas trahens
 ??? XP_013760738 237 32% 44%

Evolutionary history

C8orf82 does not belong to a specific gene family and has no other known alternative splice isoforms. This holds true for its most distant ancestor as well.[1][4]

Comparison of C8orf82's % of amino acid changes over time to that of cytochrome c and fibrinogen. Generated using data from ortholog table.

The above graph show's how the behavior of C8orf82's percent of amino acid changes over time is similar to that of fibrinogen and vastly different from cytochrome c indicating a very slow rate of change over time.

Below is a graph that depicts the percent identity change of the orthologs listed in the ortholog table based on the orthologs dates of divergence.

C8orf82's orthologs percent identity change over time based on date of divergence.

Protein

The C8orf82 gene encodes for the protein termed UPF0598 protein C8orf82 or just C8orf82.[4]

General properties

The protein is 216 amino acids long and contains a domain of unknown function DUF4505. This domain of unknown function is also known as pfam14956.[5] The protein has a molecular weight of 23.8 kDa and an isoelectric point of 9.36.[6]

Compositional features

Isoleucine (0.5%) and lysine (1.4%) within C8orf82 were found to occur in lower frequencies compared to other normal human proteins, while proline(9.7%) and arginine (10.6%) had slightly higher occurrence frequencies.[6]

Post-translational modifications

Predicted

sumoylation, O-linked glycosylation, O-ß-GlcNAc attachment sites and an NES motif was also detected.[7]

A conceptual translation of UPF0598 protein C8orf82 showing post translation modifications and secondary structure.

Subcellular localization

C8orf82 is predicted to be localized within the mitochondria using PSORT II analysis. The results of a k-NN prediction attributed 60.9% to mitochondrial localization and 26.1% to nuclear localization.[8]

Interacting proteins

ETFA has been shown to interact with C8orf82 using high-throughput affinity-purification mass spectrometry.[9]

Expression

Using

myocytes were weakly stained or negative. A staining analysis was also done on cancer cells and the results were as follows; a majority of malignant cells displayed weak to moderate positivity. Many cases of malignant carcinoids and lymphomas were negative. In all these tests, only a single antibody was used (CAB034331).[10]

Microarray data has shown C8orf82 to be ubiquitously expressed.

Microarray data for C8orf82 throughout normal human tissues.[11]

An EST profile for C8orf82 also shows ubiquitous expression.

EST profile for C8orf82 taken from Unigene.[12]

References

  1. ^ a b c d NCBI (National Center for Biotechnology Information) gene entry on C8orf82 [1]
  2. ^ NCBI Nucleotide entry on C8orf82
  3. ^ AceView entry on C8orf82
  4. ^ a b GeneCards entry on C8orf82
  5. ^ NCBI Protein entry on C8orf82
  6. ^ a b SDSC Biology WorkBench 3.2 - Statistical Analysis of Primary Structure tool [2]
  7. ^ ExPASy Proteomics Tools
  8. ^ "PSORT II Prediction Analysis". Archived from the original on 2021-07-09. Retrieved 2016-05-09.
  9. PMID 26186194
    .
  10. ^ The Human Protein Atlas entry on C8orf82
  11. ^ NCBI GEO Profile entry on C8orf82 GDS3113
  12. ^ Unigene entry for C8orf82
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