CENPA
| CENPA | |||
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Gene ontology | |||
| Molecular function | |||
| Cellular component | |||
| Biological process | |||
| Sources:Amigo / QuickGO | |||
Ensembl | |||||||||
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| UniProt | |||||||||
| RefSeq (mRNA) | |||||||||
| RefSeq (protein) | |||||||||
| Location (UCSC) | Chr 2: 26.76 – 26.8 Mb | Chr 5: 30.82 – 30.83 Mb | |||||||
| PubMed search | [3] | [4] | |||||||
| View/Edit Human | View/Edit Mouse |
Centromere protein A, also known as CENPA, is a protein which in humans is encoded by the CENPA gene.[5] CENPA is a histone H3 variant which is the critical factor determining the kinetochore position(s) on each chromosome[6] in most eukaryotes including humans.
Function
CENPA is a protein which
histone modification sites including H3K4, H3K9 and H3K27.[11]
Unusually for a histone, CENPA nucleosomes are not
repetitive satellite DNA.[16] The position of CENPA within satellite DNA are heritable at the protein level through a purely epigenetic mechanism.[17] This means that the position of CENPA protein binding to the genome is copied upon cell division to the two daughter cells independent of the underlying DNA sequence. Under circumstances in which CENPA is lost from a chromosome a fail-safe mechanism has been described in human cells in which CENPB recruits CENPA via a satellite DNA binding domain to repopulate the centromere with CENPA nucleosomes.[18]
CENPA interacts directly with the inner kinetochore through proteins including CENPC and CENPN.[19][20] Through this interaction the microtubules are able to accurately segregate chromosomes during mitosis.
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000115163 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029177 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ EntrezGene 1058
- PMID 19002142.
- PMID 23873148.
- PMID 35357911.
- PMID 11879637.
- PMID 28235947.
- PMID 29569072.
- PMID 17339380.
- S2CID 17907028.
- PMID 30503616.
- PMID 24905786.
- PMID 24763964.
- PMID 27510565.
- PMID 32959893.
- PMID 32516549.
- PMID 31578520.
External links
- Human CENPA genome location and CENPA gene details page in the UCSC Genome Browser.
Further reading
- Palmer DK, O'Day K, Trong HL, Charbonneau H, Margolis RL (May 1991). "Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone". Proceedings of the National Academy of Sciences of the United States of America. 88 (9): 3734–3738. PMID 2023923.
- Sullivan KF, Hechenberger M, Masri K (November 1994). "Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere". The Journal of Cell Biology. 127 (3): 581–592. PMID 7962047.
- Shelby RD, Vafa O, Sullivan KF (February 1997). "Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites". The Journal of Cell Biology. 136 (3): 501–513. PMID 9024683.
- Valdivia MM, Figueroa J, Iglesias C, Ortíz M (January 1998). "A novel centromere monospecific serum to a human autoepitope on the histone H3-like protein CENP-A". FEBS Letters. 422 (1): 5–9. S2CID 40104381.
- Fowler KJ, Newson AJ, MacDonald AC, Kalitsis P, Lyu MS, Kozak CA, et al. (1998). "Chromosomal localization of mouse Cenpa gene". Cytogenetics and Cell Genetics. 79 (3–4): 298–301. PMID 9605877.
- Muro Y, Azuma N, Onouchi H, Kunimatsu M, Tomita Y, Sasaki M, et al. (April 2000). "Autoepitopes on autoantigen centromere protein-A (CENP-A) are restricted to the N-terminal region, which has no homology with histone H3". Clinical and Experimental Immunology. 120 (1): 218–223. PMID 10759786.
- Lomonte P, Sullivan KF, Everett RD (February 2001). "Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ICP0". The Journal of Biological Chemistry. 276 (8): 5829–5835. PMID 11053442.
- Uren AG, Wong L, Pakusch M, Fowler KJ, Burrows FJ, Vaux DL, et al. (November 2000). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Current Biology. 10 (21): 1319–1328. S2CID 18455745.
- Zeitlin SG, Shelby RD, Sullivan KF (December 2001). "CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis". The Journal of Cell Biology. 155 (7): 1147–1157. PMID 11756469.
- Ando S, Yang H, Nozaki N, Okazaki T, Yoda K (April 2002). "CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells". Molecular and Cellular Biology. 22 (7): 2229–2241. PMID 11884609.
- Saxena A, Saffery R, Wong LH, Kalitsis P, Choo KH (July 2002). "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated". The Journal of Biological Chemistry. 277 (30): 26921–26926. PMID 12011073.
- Figueroa J, Pendón C, Valdivia MM (May 2002). "Molecular cloning and sequence analysis of hamster CENP-A cDNA". BMC Genomics. 3: 11. PMID 12019018.
- Saxena A, Wong LH, Kalitsis P, Earle E, Shaffer LG, Choo KH (September 2002). "Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc". Human Molecular Genetics. 11 (19): 2319–2329. PMID 12217960.
- Tomonaga T, Matsushita K, Yamaguchi S, Oohashi T, Shimada H, Ochiai T, et al. (July 2003). "Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer". Cancer Research. 63 (13): 3511–3516. PMID 12839935.
- Kunitoku N, Sasayama T, Marumoto T, Zhang D, Honda S, Kobayashi O, et al. (December 2003). "CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function". Developmental Cell. 5 (6): 853–864. PMID 14667408.
- Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (February 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes to Cells. 9 (2): 105–120. S2CID 21813024.
- Yasuda S, Oceguera-Yanez F, Kato T, Okamoto M, Yonemura S, Terada Y, et al. (April 2004). "Cdc42 and mDia3 regulate microtubule attachment to kinetochores". Nature. 428 (6984): 767–771. S2CID 4401953.
- Black BE, Foltz DR, Chakravarthy S, Luger K, Woods VL, Cleveland DW (July 2004). "Structural determinants for generating centromeric chromatin". Nature. 430 (6999): 578–582. S2CID 4392941.
- Sullivan BA, Karpen GH (November 2004). "Centromeric chromatin exhibits a histone modification pattern that is distinct from both euchromatin and heterochromatin". Nature Structural & Molecular Biology. 11 (11): 1076–1083. PMID 15475964.
- Sekulic N, Bassett EA, Rogers DJ, Black BE (September 2010). "The structure of (CENP-A-H4)(2) reveals physical features that mark centromeres". Nature. 467 (7313): 347–351. PMID 20739937.