Phlebotomy
The examples and perspective in this article may not represent a worldwide view of the subject. (July 2018) |
Phlebotomy | |
---|---|
ICD-9-CM | 38.99 |
MeSH | D018962 |
Phlebotomy is the process of making a puncture in a vein, usually in the arm, with a cannula for the purpose of drawing blood.[1] The procedure itself is known as a venipuncture, which is also used for intravenous therapy. A person who performs a phlebotomy is called a phlebotomist, although most doctors, nurses, and other technicians can also carry out a phlebotomy.[2] In contrast, phlebectomy is the removal of a vein.
Phlebotomies that are carried out in the treatment of some blood disorders are known as therapeutic phlebotomies.[3] The average volume of whole blood drawn in a therapeutic phlebotomy to an adult is 1 unit (450–500 ml) weekly to once every several months, as needed.[4]
Etymology
From
Phlebotomies
Phlebotomies are carried out by phlebotomists – people trained to draw blood mostly from veins for clinical or medical testing, transfusions, donations, or research. Blood is collected primarily by performing
A therapeutic phlebotomy may be carried out in the treatment of some blood disorders (example: Hemochromatosis, polycythemia vera, porphyria cutanea tarda), and chronic hives (in research).[8][9]
Australia
In Australia, there are a number of courses in phlebotomy offered by educational institutions, but training is typically provided on the job. The minimum primary qualification for phlebotomists in Australia is a Certificate III in Pathology Collection (HLT37215) from an approved educational institution.[10]
United Kingdom
In the
United States
Special state certification in the
South Africa
In South Africa learnerships to qualify as a Phlebotomy Technician are offered by many public and private educational institutions as well as by private academies owned up by pathology laboratories (such as Ampath Laboratories, Lancet, PathCare) and healthcare service providers (such as Netcare, South African National Blood Service). Some of the larger retail pharmacy chains offering in-store clinical services (such as Clicks, Dis-Chem) also provide training for aspirant phlebotomists. Certification can be obtained from a number of examination and testing institutions. To work as a phlebotomist in South Africa, registration with the Health Professions Council of South Africa (HPCSA) is required.
Sample-tube-types
Tube cap color or type in order of draw | Additive | Usage and comments |
---|---|---|
Blood culture bottle | Sodium polyanethol sulfonate ( microorganisms |
Usually drawn first for minimal risk of contamination.[13] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms.[14] |
Blue
("light blue") |
Sodium citrate
(weak calcium chelator/anticoagulant) |
Coagulation tests such as prothrombin time (PT) and partial thromboplastin time (PTT) and thrombin time (TT). Tube must be filled 100%. |
Plain red | No additive | Serum: cryoglobulins
|
Gold (sometimes red and grey "tiger top"[15]) | Clot activator and serum separating gel[16] | Serum-separating tube (SST): Tube inversions promote clotting. Most chemistry, endocrine and serology tests, including hepatitis and HIV. |
Orange | Clot activator and serum separating gel[17] | Rapid serum-separating tube (RST). |
Dark green | Sodium heparin (anticoagulant) | Chromosome testing, HLA typing, ammonia, lactate |
Light green | Lithium heparin (anticoagulant)
Plasma separator gel |
Plasma. Tube inversions prevent clotting |
Lavender ("purple") | EDTA (chelator / anticoagulant) | cyclosporin
|
Pink | K2 EDTA ( chelator / anticoagulant ) |
viral load |
Royal blue
("navy") |
EDTA ( chelator / anticoagulant ) |
Trace elements, heavy metals, most drug levels, toxicology |
Tan | Sodium EDTA ( chelator / anticoagulant ) |
Lead |
Gray |
Fluoride Oxalate
|
Glucose, lactate,[19] toxicology[20] |
Yellow | Acid-citrate-dextrose A (anticoagulant) | Tissue typing, DNA studies, HIV cultures |
Pearlescent ("white") | Separating gel and (K2)EDTA | toxoplasma and HHV-6
|
Black | Sodium Citrate | Paediatric ESR
|
QuantiFERON
Grey, Green, Yellow, Purple |
QuantiFERON
1. Grey (nil) tube 2. Green (TB1 antigen) tube 3. Yellow (TB2 antigen) tube 4. Purple (mitogen) tube |
Tuberculosis |
History
Early phlebotomists used techniques such as
See also
- Cytotechnologist
- Injection (medicine)
- Medical technologist
- List of surgeries by type
- Phlebotominae
- Phlebotomy licensure
References
- ISBN 978-1-947172-04-3.
- ^ "FAQ". National Association of Phlebotomists. Archived from the original on 28 December 2014. Retrieved 28 December 2014.
- PMID 27486346.
- ^ Retrieved 17 February 2022.
- PMID 26524965.
- PMID 21391461.
- ^ "Best practices in phlebotomy". WHO Guidelines on Drawing Blood. World Health Organization. 2010.
- PMID 20228645.
- PMID 31139656.
- ^ "Certificate III in Pathology Collection". Australian Government. Archived from the original on 14 January 2018. Retrieved 14 January 2018.
- ^ "Phlebotomist". NHS Careers. Archived from the original on 29 September 2014. Retrieved 15 September 2014.
- ^ "Archived copy" (PDF). Archived from the original (PDF) on 2016-03-23. Retrieved 2016-03-16.
{{cite web}}
: CS1 maint: archived copy as title (link) - ISBN 978-0-323-22592-2.
- ISBN 978-1-55581-881-4.
- ^ "Test Tube Guide and Order of Draw" (PDF). Guthrie Laboratory Services. June 2019.
- ^ "Specimen requirements/containers". Pathology & Laboratory Medicine, UCI School of Medicine. Retrieved 2020-09-10.
- ^ "Proper Order of Draw" (PDF). Memorial Lab Services. Retrieved 2023-06-15.
- PMID 1644639.
- ISBN 978-0-12-813777-2.
- ^ "What types of samples will the Toxicology Lab analyze?". Oklahoma State Bureau of Investigation. Retrieved 2024-01-24.