E-box
An E-box (enhancer box) is a
Discovery
The E-box was discovered in a collaboration between
In 1989, David Baltimore's lab discovered the first two E-box binding
Since then, researchers have established that the E-box affects gene
Binding
E-box binding proteins play a major role in regulating transcriptional activity. These proteins usually contain the
The E-box binding is modulated by Zn2+ in mice. The CT-Rich Regions (CTRR) located about 23
The binding specificity of different E-boxes is found to be essential in their function. E-boxes with different functions have a different number and type of binding factor.[12]
The consensus sequence of the E-box is usually CANNTG; however, there exist other E-boxes of similar sequences called noncanonical E-boxes. These include, but are not limited to:
- CACGTT sequence 20 bp upstream of the mouse Period2 (PER2) gene and regulates its expression[13]
- CAGCTT sequence found within the MyoD core enhancer[14]
- CACCTCGTGAC sequence in the lipoproteins.[15]
Role in the circadian clock
The link between E-box-regulated genes and the
The E-box plays an important role in circadian genes; so far, nine E/E'BOX controlled circadian genes have been identified:
The E-box is one of the top five transcription factor families associated with the circadian phase and is found in most tissues.
E-box like CLOCK-related elements (EL-box; GGCACGAGGC) are also important in maintaining circadian rhythmicity in clock-controlled genes. Similarly to the E-box, the E-box like CLOCK related element can also induce transcription of BMAL1/CLOCK, which can then lead to expression in other EL-box containing genes (Ank, DBP, Nr1d1).
Both non-canonical E-boxes and E-box-like sequences are crucial for circadian oscillation. Recent research on this forms an hypothesis that either a canonical or non-canonical E-box followed by an E-box like sequence with 6 base pair interval in between is a necessary combination for circadian transcription.[21] In silico analysis also suggests that such an interval existed in other known clock-controlled genes.
Role of proteins which bind to E-boxes
There are several proteins that bind to the E-box and affect gene transcription.
CLOCK-ARNTL complex
The CLOCK-
Knowing that binding activates transcription of the per gene in the promoter region, researchers discovered in 2002 that
In 2006, Ripperger and Schibler discovered that the binding of this complex to the E-box drove circadian
MYC (c-Myc, an oncogene)
MYC (
In 1991, researchers tested whether c-Myc could bind to DNA by dimerizing it to E12. Dimers of E6, the chimeric protein, were able to bind to an E-box element (GGCCACGTGACC) which was recognized by other HLH proteins.[24] Expression of E6 suppressed the function of c-Myc, which showed a link between the two.
In 1996, it was found that Myc heterodimerizes with MAX and that this heterodimeric complex could bind to the CAC(G/A)TG E-box sequence and activate transcription.[25]
In 1998, it was concluded that the function of c-Myc depends upon activating transcription of particular genes through E-box elements.[26]
MYOD1 (MyoD)
MyoD comes from the Mrf
When MyoD binds to the E-box motif CANNTG, muscle differentiation and expression of muscle-specific proteins is initiated.[27] The researchers ablated various parts of the recombinant MyoD sequence and concluded that MyoD used encompassing elements to bind the E-box and the tetralplex structure of the promoter sequence of the muscle specific gene α7 integrin and sarcomeric sMtCK.
MyoD regulates HB-EGF (
MyoD can also bind to noncanonical E boxes of MyoG and regulate its expression.[28]
MyoG (Myogenin)
MyoG belongs to the MyoD transcription factor family. MyoG-E-Box binding is necessary for
MyoG and MyoD have also been shown to involve in
TCF3 (E47)
E47 is produced by alternative spliced E2A in E47 specific bHLH-encoding
Similar to other E-box binding proteins, E47 also binds to the CANNTG sequence in the E-box. In homozygous E2A knock-out mice,
Recent research
Although the structural basis for how BMAL1/CLOCK interact with the E-box is unknown, recent research has shown that the
Researchers at the Medical School of Nanjing University found that the amplitude of FBXL3 (F-box/Leucine rich-repeat protein) is expressed via an E-box.[40] They studied mice with FBXL3 deficiency and found that it regulates feedback loops in circadian rhythms by affecting circadian period length.
A study published April 4, 2013 by researchers at
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External links
- E-Box+Elements at the U.S. National Library of Medicine Medical Subject Headings (MeSH)