RPMI 1640

RPMI 1640, simply known as RPMI medium, is a

cells in suspension cultures, but can also support a wide variety of adherent cells.

It was originally developed to culture human leukemic cells. Over the years, the original formulation was modified and refined by researchers and commercial suppliers to enhance its ability to support the growth of many cell types. This medium contains a great deal of

.

Composition

Many different formulations exist. Typically, one liter of RPMI 1640 contains:[2]

• Glucose (2 g)
• A pH indicator (phenol red, 5 mg)
• Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
• Amino acids (300 mg glutamine; 200 mg arginine; 50 mg each asparagine, cystine, leucine, and isoleucine; 40 mg lysine hydrochloride; 30 mg serine; 20 mg each aspartic acid, glutamic acid, hydroxyproline, proline, threonine, tyrosine, and valine; 15 mg each histidine, methionine, and phenylalanine; 10 mg glycine; 5 mg tryptophan; and 1 mg reduced glutathione)
• Vitamins (35 mg i-inositol; 3 mg choline chloride; 1 mg each para-aminobenzoic acid, folic acid, nicotinamide, pyridoxine hydrochloride, and thiamine hydrochloride; 0.25 mg calcium pantothenate; 0.2 mg each biotin and riboflavin; and 0.005 mg cyanocobalamin)

References

External links

• http://www.bioind.com/RPMI1640
• http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Product_Lines/Classic_Media___Salts/RPMI_Media.html
• http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI.html
• http://www.captivatebio.com/rpmi-1640-medium.html