Thayer–Martin agar
Thayer–Martin agar (or Thayer–Martin medium, or VPN agar) is a
antibiotics. It is used for culturing and primarily isolating pathogenic Neisseria bacteria, including Neisseria gonorrhoeae and Neisseria meningitidis, as the medium inhibits the growth of most other microorganisms. When growing Neisseria meningitidis, one usually starts with a normally sterile body fluid (blood or CSF), so a plain chocolate agar is used. Thayer–Martin agar was initially developed in 1964, with an improved formulation published in 1966.[1][2][3]
Components
It usually contains the following combination of antibiotics, which make up the VPN acronym:
- Gram-positive organisms, although some Gram-positive organisms such as Lactobacillus and Pediococcusare intrinsically resistant
- Gram-negative organisms except Neisseria, although some other Gram-negative organisms such as Legionellaare also resistant
- fungi
- Trimethoprim inhibits swarming of Proteus spp[4]
Clinical implications
A negative culture on Thayer–Martin in a patient exhibiting symptoms of pelvic inflammatory disease most likely indicates an infection with Chlamydia trachomatis. [citation needed]
References
- ^ "THAYER-MARTIN Agar (Base)".
- PMID 14105729.
- PMID 4957043.
- ^ Tankeshwar, Acharya (2016-06-13). "Modified Thayer-Martin Agar: Composition, preparation, uses and colony characteristics". Learn Microbiology Online. Retrieved 2020-10-28.