Hygromycin B
Clinical data | |
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Trade names | Hygromix |
Other names | O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine , HYG |
AHFS/Drugs.com | International Drug Names |
ATC code |
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Identifiers | |
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JSmol) | |
Melting point | 160 to 180 °C (320 to 356 °F) (decomp.) |
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Hygromycin B is an
protein synthesis.[1]
History
Hygromycin B was originally developed in the 1950s for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: Hygromix). Hygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980s.[2][3]
Mechanism of action
Hygromycin is active against both
polypeptide synthesis. It stabilizes the tRNA-ribosomal acceptor site, thereby inhibiting translation.[citation needed
]
Use in research
In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin
resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.[4] Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells. Based on impurity monitor method,[5] four different kinds of impurities are discovered in commercial hygromycin B from different suppliers and toxicities of different impurities to the cell lines are described in the following external links.[citation needed
]
Use in plant research
Hygromycin
transformants.[6]
Fungus Coniothyrium minitans was transformed with the hygromycin B resistance gene to improve the infection rates of Sclerotinia sclerotiorum, a fungal parasite of many crops.[7]
References
- PMID 24542808.
- PMID 6319235.
- PMID 6314265.
- PMID 6318654.
- ^ Kauffman JS (2009). "Analytical Strategies for Monitoring Residual Impurities Best methods to monitor product-related impurities throughout the production process". BioPharm International. 23: 1–3.
- S2CID 84495391. Retrieved November 17, 2014.
- PMID 12825495.