Pyranose oxidase
| pyranose oxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
ExPASy NiceZyme view | | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In
- D-glucose + O2 2-dehydro-D-glucose + H2O2
Thus, the two
2-dehydro-D-glucose and H2O2
.
Pyranose oxidase is able to oxidize
D-glucono-1,5-lactone, which have the same ring conformation and configuration at C-2, C-3 and C-4.[2]
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is pyranose:oxygen 2-oxidoreductase. Other names in common use include glucose 2-oxidase, and pyranose-2-oxidase. This enzyme participates in pentose phosphate pathway. It employs one cofactor, FAD.
Structural studies
As of late 2007, 8
structures have been solved for this class of enzymes, with PDB accession codes 1TT0, 1TZL, 2F5V, 2F6C, 2IGK, 2IGM, 2IGN, and 2IGO
.
Use in biosensors
Recently, pyranose oxidase has been gaining on popularity within biosensors.E. coli.[1]
References
Further reading
- Machida Y, Nakanishi T (1984). "Purification and properties of pyranose oxidase from Coriolus versicolor". Agricultural and Biological Chemistry. 48 (10): 2463–2470. .
- Neidleman SL, Amon Jr WF, Geigert J (1981). "Process for the production of fructose". Chem. Abstr. 94: 20737.
- Ruelius HW, Kerwin RM, Janssen FW (November 1968). "Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. I. Isolation and purification". Biochimica et Biophysica Acta (BBA) - Enzymology. 167 (3): 493–500. PMID 5725162.
