Humanized antibody
Humanized antibodies are
Humanized antibodies are distinct from
There are other ways to develop monoclonal antibodies. This
Use of recombinant DNA in humanization process
The humanization process takes advantage of the fact that production of monoclonal antibodies can be accomplished using
Distinction from "chimeric antibody"
Humanization is usually seen as distinct from the creation of a mouse-human antibody chimera. So, although the creation of an antibody chimera is normally undertaken to achieve a more human-like antibody (by replacing constant region of the mouse antibody with that from human) simple chimeras of this type are not usually referred to as humanized. Rather, the protein sequence of a humanized antibody is essentially identical to that of a human variant, despite the non-human origin of some of its complementarity-determining region (CDR) segments responsible for the ability of the antibody to bind to its target antigen.
Chimeric antibody names contain a -xi- stem. Examples of chimeric antibodies approved for human therapy include abciximab (ReoPro), basiliximab (Simulect), cetuximab (Erbitux), infliximab (Remicade) and rituximab (MabThera). There are also several examples of chimerics currently in clinical trials (e.g. bavituximab, see sortable list for additional examples).
Humanizing via a chimeric intermediate
The humanization process may also include the creation of a mouse-human chimera as an initial step. In this case, a mouse variable region is spliced to a human constant region. The chimera can then be further humanized by selectively altering the sequence of amino acids in the variable region of the molecule.
The alteration process must be "selective" to retain the specificity for which the antibody was originally developed. That is, since the CDR portions of the variable region are essential to the ability of the antibody to bind to its intended target, the amino acids in these portions cannot be altered without the risk of undermining the purpose of the development. Aside from the CDR segments, the portions of the variable regions that differ from those in humans can be corrected by exchanging the appropriate individual amino acids. This is accomplished at the DNA level through mutagenesis.
Naming of humanized chimeras includes the stem for both designations (-xi- + -zu-).
Humanization by insertion of relevant CDRs into human antibody "scaffold"
It is possible to produce a humanized antibody without creating a chimeric intermediate. "Direct" creation of a humanized antibody can be accomplished by inserting the appropriate CDR coding segments (so-called 'donor', responsible for the desired binding properties) into a human antibody "scaffold" (so-called 'acceptor'). As discussed above, this is achieved through recombinant DNA methods using an appropriate vector
Alemtuzumab is an early example of an antibody whose humanization did not include a chimeric intermediate. In this case, a monoclonal dubbed "Campath-1" was developed to bind CD52 using a mouse system. The hypervariable loops of Campath-1 (that contain its CDRs and thereby impart its ability to bind CD52) were then extracted and inserted into a human antibody framework.[1] Alemtuzumab is approved for treatment of B-cell chronic lymphocytic leukemia[11] and is currently in clinical trials for a variety of other conditions including multiple sclerosis.[12]
Derivation from sources other than mice
There are technologies that completely avoid the use of mice or other non-human mammals in the process of discovering antibodies for human therapy. Examples of such systems include various "display" methods (primarily phage display) as well as methods that exploit the elevated B-cell levels that occur during a human immune response.
Display methods
These employ the selective principles of specific antibody production but exploit micro-organisms (as in
This means that, although antibody fragments created using display methods are of fully human sequence, they are not full antibodies. Therefore, processes in essence identical to humanization are used to incorporate and express the derived affinities within a full antibody.
Adalimumab (Humira) is an example of an antibody approved for human therapy that was created through phage display.[13][14]
Antibodies from human patients or vaccine recipients
It is possible to exploit human immune reaction in the discovery of monoclonal antibodies. Simply put, human immune response works in the same way as that in a mouse or other non-human mammal. Therefore, persons experiencing a challenge to their immune system, such as an infectious disease, cancer or a vaccination are a potential source of monoclonal antibodies directed at that challenge. This approach seems especially apt for the development of anti-viral therapies that exploit the principles of passive immunity. Variants of this approach have been demonstrated in principle[15] and some are finding their way into commercial development.[16]
See also
References
- ^ S2CID 4335569.
- PMID 2513570. (This is an early example of the use of the term "humanized antibody".)
- ^ PMID 9202712.
- ^ Clinical Trials page list for otelixizumab
- PMID 15848072.
- PMID 18424812.
- PMID 27667400.
- PMID 28165915.
- PMID 36594750.
- ^ Fletcher, L. PDL's mAb technology finds right timing. Nat Biotechnol 19, 395–396 (2001). https://doi.org/10.1038/88010
- ^ DrugBank entry for alemtuzumab
- ^ Clinical Trials pages for alemtuzumab
- PMID 10577977.
- PMID 12379628.
- PMID 14659916.
- ^ http://www.theraclone-sciences.com/pdf/Theraclone_ISTAR.pdf Archived 2016-03-03 at the Wayback Machine Example of method using human patients as source of monoclonal antibodies