Pfu DNA polymerase
Pfu DNA polymerase | |||||||
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Identifiers | |||||||
Organism | |||||||
Symbol | pol | ||||||
UniProt | P61875 | ||||||
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Pfu DNA polymerase is an enzyme found in the hyperthermophilic
It is a family B
Proofreading ability of Pfu polymerase
Pfu DNA polymerase has superior thermostability and proofreading properties compared with
Commercially available Pfu typically results in an error rate of 1 in 1.3 million base pairs and can yield 2.6% mutated products when amplifying 1 kb fragments using PCR. However, Pfu is slower and typically requires 1–2 minutes per cycle to amplify 1kb of DNA at 72 °C. Using Pfu DNA polymerase in PCR reactions also results in blunt-ended PCR products.[2]
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.[3]
History
Scientists led by Eric Mathur at the biotech company Stratagene, based in
Other polymerases from Pyrococcus strains such as "Deep Vent" (Q51334) from strain GB-D and Pwo DNA polymerase has also seen use.[3]
References
- ^ InterPro protein view: P61875
- ^ Agilent Technologies. "PfuTurbo DNA Polymerase Instruction Manual #600250" (PDF).
- ^ ISBN 978-1-4020-6240-7.
- PMID 1761218.
- ^ U.S. patent 5,489,523, U.S. patent 5,545,552