Vancomycin-resistant Enterococcus
Vancomycin-resistant Enterococcus | |
---|---|
Other names | Vancomycin-resistant enterococci |
SEM micrograph of vancomycin-resistant enterococci | |
Specialty | Microbiology |
Prevention | Screen with peri-rectal swab[1] |
Treatment | Linezolid [2] |
Vancomycin-resistant Enterococcus, or vancomycin-resistant enterococci (VRE), are
Mechanism of acquired resistance
Six different types of vancomycin resistance are shown by enterococcus: Van-A, Van-B, Van-C, Van-D, Van-E and Van-G.[4] The significance is that Van-A VRE is resistant to both vancomycin and teicoplanin,[5] Van-B VRE is resistant to vancomycin but susceptible to teicoplanin,[6][7] and Van-C is only partly resistant to vancomycin.
The mechanism of resistance to vancomycin found in enterococcus involves the alteration of the peptidoglycan synthesis pathway.[8]
The D-alanyl-D-lactate variation results in the loss of one hydrogen-bonding interaction (four, as opposed to five for D-alanyl-D-alanine) being possible between vancomycin and the peptide. The D-alanyl-D-serine variation causes a six-fold loss of affinity between vancomycin and the peptide, likely due to steric hindrance.[9][10]
To become vancomycin-resistant, vancomycin-sensitive enterococci typically obtain new DNA in the form of
Diagnosis
Once the individual has VRE, it is important to ascertain which strain.[14]
Screening
Screening for VRE can be accomplished in a number of ways. For inoculating peri-rectal/anal swabs or stool specimens directly, one method uses bile esculin azide agar plates containing 6 μg/ml of vancomycin. Black colonies should be identified as an enterococcus to species level and further confirmed as vancomycin resistant by an MIC method before reporting as VRE.[1]
Vancomycin resistance can be determined for enterococcal colonies available in pure culture by inoculating a suspension of the organism onto a commercially available brain heart infusion agar (BHIA) plate containing 6 μg/ml vancomycin. The Clinical and Laboratory Standards Institute (CLSI) recommends performing a vancomycin MIC test and also motility and pigment production tests to distinguish species with acquired resistance (vanA and vanB) from those with vanC intrinsic resistance.[1] Detection of vancomycin resistance by the use of PCR targeting vanA and vanB can also be performed.[15][16]
Treatment of infection
History
High-level vancomycin-resistant
See also
- Vancomycin-resistant Staphylococcus aureus (VRSA)
- Antibiotic resistance
- Drug resistance
- MDR-TB
References
- ^ a b c "Vancomycin-resistant Enterococci (VRE) and the Clinical Laboratory". Centers for Disease Control and Prevention. Retrieved 21 May 2017. This article incorporates text from this source, which is in the public domain.
- ^ PMID 24247127.
- ^ "Vancomycin-resistant Enterococci (VRE) in Healthcare Settings". VRE in Healthcare Settings - HAI. CDC. Retrieved 2015-06-09.
- ISBN 9780387724188.
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- ^ "VRE and the Clinical Laboratory - HAI". Healthcare-associated Infections. CDC. Retrieved 2015-06-09.
- PMID 30020605. Retrieved 27 March 2022.
- PMID 16610573.
The LightCycler instrument (Roche Diagnostics Corporation) is used to detect vanA and vanB using a rapid real-time PCR assay(Figure 3). This method is more sensitive and faster (~3.5 vs >72 hours) than culture for detecting VRE colonization.47 The assay detects the presence of genes associated with vancomycin resistance in enterococci, vanA and vanB.
- PMID 28203378.
- PMID 22669234.
- ISBN 9783527676156.
- PMID 25304643.
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- S2CID 221144533.
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- ISBN 9780470600115.
- PMID 10706902.
The first reports of vancomycin-resistant enterococci (later classified as VanA type of resistance) involved strains of E. faecium that were resistant to vancomycin and teicoplanin (another glycopeptide) and that were isolated from patients in France and England in 1986. Vancomycin-resistant E. faecalis, subsequently classified as VanB type, was recovered from patients in Missouri in 1987.
- S2CID 205988392.
- ^ "Diseases and Organisms in Healthcare Settings". Healthcare-associated Infections (HAIs). CDC. Retrieved 2015-06-09.
- PMID 23115435.
- PMID 22957131.
- ^ Fathollahzadeh B, Hashemi FB, Emaneini M, Aligholi M, Nakhjavani FA (2006). "Frequency of Vancomycin-Resistant Enterococci from Three Hospitals in Iran". Daru. 14 (3): 141–146. Archived from the original (PDF) on 2016-03-04. Retrieved 2015-12-30.
Further reading
- Rastall B, Gibson G (2006-05-01). Prebiotics: Development and Application. John Wiley & Sons. ISBN 9780470023143.