Polyneuridine-aldehyde esterase

polyneuridine-aldehyde esterase
ExPASy		NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
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The enzyme polyneuridine-aldehyde esterase (EC 3.1.1.78) catalyzes the following reaction:^[1]

polyneuridine aldehyde + H₂O ${\displaystyle \rightleftharpoons }$ 16-epivellosimine + CO₂ + methanol

This enzyme participates in indole and ipecac alkaloid biosynthesis.

Nomenclature

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name is polyneuridine aldehyde hydrolase (decarboxylating). Other names in common use include:

• polyneuridine aldehyde esterase
• PNAE

Homologues

This enzyme is found in various forms in plant species such as

(tomato) among others.

Polyneuridine-aldehyde esterase also appears in select bacteria including Enterobacter cloacae.

Structure

The secondary structure of this enzyme consists mainly of

. In its native form, this enzyme has a tertiary structure that includes two main lobes (as depicted above in the blue 3D representation on the top right).

Reaction

${\displaystyle {\xrightarrow[{+H_{2}O\ -CH_{3}OH}]{polyneuridine-aldehyde\ esterase}}}$

${\displaystyle {\xrightarrow[{-CO_{2}}]{}}}$

Polyneuridine-aldehyde esterase catalyzes the

Mechanism

product 16-epi-vellosimine that is depicted as a space-filling model (carbon = white, oxygen = red, nitrogen = blue).^[2]

The mechanism of hydrolysis performed by polyneuridine-aldehyde esterase is not known. It has been suggested that the enzyme utilizes a

Broader significance

This enzyme is a part of the pathway of indole alkaloid biosynthesis. The indole alkaloids that result from this metabolic pathway are used by many plant species as a defense against herbivores and parasites.

Open questions

The precise mechanisms by which this enzyme performs its function is still unknown. As noted above, researchers are formulating suggestions as to how polyneuridine-aldehyde esterase catalyses the decomposition of polyneuridine-aldehyde, but a mechanism has not yet been affirmed with absolute certainty. Due to the lack of complete understanding of polyneuridine-aldehyde esterase's precise mechanism, this enzyme cannot be grouped into a family of enzymes. Based on mechanism theories, suggestions can be made as to how this enzyme should be categorized, and some parallels can be drawn between polyneuridine-aldehyde esterase and other enzymes.

References

Further reading

• Pfitzner A, Stockigt J (1983). "Polyneuridine aldehyde esterase: an unusual specific enzyme involved in the biosynthesis of sarpagine type alkaloids". Journal of the Chemical Society, Chemical Communications (8): 459–460.
  doi:10.1039/C39830000459
  .