Clostridioides difficile

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This article is about the bacterium. For the disease, see Clostridioides difficile infection.

Clostridioides difficile
C. difficile colonies on a blood agar plate
Electron micrograph of the bacterium
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Bacillota
Class: Clostridia
Order: Eubacteriales
Family: Peptostreptococcaceae
Genus: Clostridioides
Species:
C. difficile
Binomial name
Clostridioides difficile
(Hall & O'Toole, 1935) Lawson & Rainey, 2016
Synonyms
  • Bacillus difficilis Hall & O'Toole, 1935[1]
  • Clostridium difficile (Hall & O'Toole, 1935) Prévot, 1938[2]
  • Peptoclostridium difficile [3]

Clostridioides difficile (

spores that are able to tolerate extreme conditions that the active bacteria cannot tolerate.[8]

Clostridioides difficile is an important emerging human

fluoroquinolones and cephalosporins.[11][12][13] The majority of infections are acquired outside of hospitals, and most antibiotics have similar elevated risk of infection on par with many non-antibiotic risk factors, such as using stool softeners and receiving an enema.[14]

Clostridioides difficile can also become established in the human

laxative abuse.[16] Individuals with no history of gastrointestinal disturbances appear unlikely to become asymptomatic carriers. These carriers are thought to be a major reservoir of infection.[17]

Taxonomy

The species was transferred from the genus Clostridium to Clostridioides in 2016, thus giving it the binomial Clostridioides difficile.[18][19][20] This new name reflects the taxonomic differences between this species and members of the genus Clostridium, while maintaining the common name as C. diff.[19] As of 2018, the only other species in this new genus is Clostridioides mangenotii (formerly known as Clostridium mangenotii).[21]

Human pathogen

Pathogenic C. difficile strains produce multiple

C. difficile colitis), although their relative contributions have been debated. The diarrhea may range from a few days of intestinal fluid loss to life-threatening pseudomembranous colitis, which is associated with intense inflammation of the colon and formation of pseudomembranes on the intestinal mucosal surface.[8] This may progress to toxic megacolon, a severe form of colonic distention that can put a patient at risk for colon perforation, sepsis and shock. Toxins A and B are glucosyltransferases that target and inactivate the Rho family of GTPases. Toxin B (cytotoxin) induces actin depolymerization by a mechanism correlated with a decrease in the ADP-ribosylation of the low molecular mass GTP-binding Rho proteins.[23] There is also a binary toxin (AB toxin), but its role in disease is not fully understood.[24]

Additional virulence factors include an adhesion factor that mediates the binding to human colonic cells and a

para-cresol, which inhibits the growth of other microbes in its vicinity and allows it to outcompete normal human gut flora.[26]

Antimicrobial resistance

Centers for Disease Control in Atlanta warned of the emergence of an epidemic strain with increased virulence, antibiotic resistance, or both.[28] Resistance to other antibiotics such as metronidazole, the first choice of antimicrobial drug when treating CDI, has been observed in up to 12% of clinical isolates, so as treatment with various antibiotics continues, more diverse and stronger resistances will continue to evolve in C. difficile populations, further complicating attempts at effective treatment.[29]

Disinfecting surfaces in hospitals may also be challenging as C. difficile spores resist many

disinfectants,[30] including high concentrations of bleach.[31]

Transmission

Gram-stained C. diff amongst other microflora from infected patient fecal sample suspension.

Clostridioides difficile is transmitted from person or animal to person by the

fecal-oral route, shed in faeces. The organism forms heat-resistant aero-tolerant spores that are not killed by alcohol-based hand cleansers or routine surface cleaning, thus, these spores survive in clinical environments for long periods.[32] Any surface, device, or material (e.g., toilets, bathing tubs, and electronic rectal thermometers) that becomes contaminated with faeces may serve as a reservoir for the C. difficile spores, and C. difficile spores can live for long periods of time on surfaces.[33] Because of this, the bacterium may be cultured from almost any surface. Once spores are ingested, their acid resistance allows them to pass through the stomach unscathed. They germinate and multiply into vegetative cells in the colon upon exposure to bile acids. Consequently, the World Health Organization advocates the use of soap in addition to alcohol solutions to limit the spread of the spores.[34] Sporulation was shown to be significantly reduced after inactivation of C. diffiicile's DNA methyltransferase CamA,[35]
raising the prospect of developing a drug that may inhibit this bacterium in a specific manner.

Susceptibility to colonization appears to be triggered by diarrheal illnesses, such as

food poisoning, Crohn's disease or laxative abuse; people without a history of diarrheal illnesses who are exposed to C. difficile spores will not necessarily become carriers.[16] Once people are colonized by C. difficile, they remain carriers for year-long time scales, but the day-to-day abundance of C. difficile fluctuates considerably - fluctuating from being below the limit of detection to high levels of shedding from one day to the next. GI disturbances in carriers appear to trigger periods of increased shedding which may be an important factor for transmission.[citation needed
]

Host range

Clostridioides difficile infects pigs, calves, and humans, and inhabits a natural reservoir of soil, faeces of domestic animals and humans, sewage, the human intestinal tract, and retail meat.[36]

A 2015 CDC study estimated that C. diff afflicted almost half a million Americans and caused 29,000 deaths in 2011. The study estimated that 40% of cases began in nursing homes or community health-care settings, while 24% occurred in hospitals.[37]

Clostridioides difficile is common in the human digestive system. However, it is a poor competitor, and is often outcompeted for nutrients by other bacteria in the digestive system. As a result, C. difficile is kept to a manageable number. If the sudden introduction of an antibiotic disrupts the microbiome, C. difficile may be able to grow as a result of many of its competitors being killed off. The incubation period is 5–10 days, with a range of 1 day to weeks following antibiotic treatment for antibiotic associated diarrhea. Additionally, carriage of C. difficile with high levels of toxins is common in young children, while disease is rare. The production of one or even both toxins is not always sufficient for producing symptoms.[38]

Signs and symptoms

Symptoms of C. difficile infection include

bowel movements a day), dehydration, abdominal pain that can be severe, loss of appetite, and nausea.[39]

Pathophysiology

C. difficile is transmitted through the oral-fecal route, and many reproduce through spores. The germination of these spores depends on the ability to sense primary bile acids in the liver, like taurocholate, which are sensed by the germinant receptor CspC. Secondary bile acids can inhibit these processes in the colon. Spores can grow and colonize the intestine by antibiotic-induced shifts in the host microbiota. C. difficile secretes mucolytic enzymes like CWp84 to degrade the colonic mucosa. These spores are also capable of adhering to colon cells. Additionally, C. difficile is a motile bacterium that can switch between both the motile and sessile phases, a process regulated by cyclic-di-GMP. On top of that, C. difficile is capable of forming biofilms and cell-to-cell signaling.[40] C. difficile is often transferred from other patients through the hands of healthcare workers or from the overall hospital environment and acquired from ingesting the pathogen. The spores resist the stomach acidity and germinate into their vegetative form in the small intestine. C. difficile can carry a broad spectrum of clinical manifestations, from being asymptomatic to severe colitis and death.[41] As such, C. difficile is the most prevalent U.S. healthcare infection, posing serious health risks and substantial care costs.[42]

Host immune response

The C. difficile secreted toxins A (TcdA) and B (TcdB), contain immunogenic antigens that are recognised by antibodies and T cells. However, the levels of anti-TcdA and -TcdB IgG antibodies have not been able to discriminate healthy individuals from patients with C. difficile infection, meaning they have limited clinical use.[43][44] Recent work has shown these toxins are also recognised by helper CD4+ T cells, predominantly by the Th17 helper cells, which are important in maintaining a healthy gut environment, although in patients with severe infection these cells are impaired.[45] Interestingly, individuals with severe C. difficile infection had significantly more toxin-specific T cells compared to those with mild infection, indicating T cells are playing a key role in fighting this infection. This immune response can further dysregulate microRNA expression.[46] This is further evidenced by the recovery of the toxin-specific Th17 cells and microRNA expression following Fecal microbiota transplant of patients with severe disease.[46][47] New findings show that the loss of the interleukin-10 corresponds to higher levels of interleukin-22, which has been found to be important in a host's response to a C.difficile infection. Thus, IL-10 deficiency can increase a host's defense against the pathogen. This could be of particular interest in future research for treatments. [48]

Diagnosis

A C. difficile infection is often suspected to be caused by foul-smelling diarrhea, but it is not confirmed if the patient has a CDI. To confirm a CDI, a cytotoxin assay detects the cell's toxin B (ToxB) cytotoxicity in the fecal eluate. The presence of C. difficile toxin is confirmed by the anti-toxin antibodies' neutralization of the cytotoxic effect. C. difficile strains can also be cultured before conducting a cytotoxin assay. These cultures detect the C. difficile strain that can produce toxins.[49] However, these enzyme immunoassays are more widely used due to their rapid turnaround, low cost, and simplicity. Additionally, they show lower sensitivity than toxigenic stool cultures. PCR assays have a shorter turnaround time and a higher sensitivity range than the toxigenic stool culture. Using a PCR-based assay helps avoid detection of asymptomatic patients. [41]

Treatment

Patients being treated with antibiotics when symptoms begin should stop taking them, if possible. This break in antibiotic therapy can sometimes lead to spontaneous resolution of symptoms. Patients who do not respond to the cessation of broad-spectrum antibiotics will need to be treated with antibiotics capable of killing C. difficile spores. Primary infections are typically treated with vancomycin, with a usual dosage of 125 mg every 6 hours.[50] The vancomycin regimen has replaced the traditional use of metronidazole due to its greater efficacy, safety profile, and lower recurrence rates. In patients who cannot tolerate vancomycin, fidaxomicin is an acceptable option with similar efficacy and even lower recurrence rates than vancomycin.[51] In cases of fulminant CDI, adjuvant therapy with parenteral metronidazole plus oral vancomycin or fidaxomicin is suggested.[52]

Approximately 15-30%[53] of patients who successfully complete therapy of primary infection with metronidazole or vancomycin will experience a relapse. About 40% of these patients will continue to have recurrent C. difficile infection. The first relapse of C. difficile is usually treated with the same antibiotic used to treat the primary infection. Any subsequent infections should not be treated with metronidazole. Occasionally, a standard 10-day course of oral vancomycin will not work. In these cases, a vancomycin taper is the preferred treatment. Patients take decreasing doses of vancomycin over a period of up to 3 months, depending on the severity of the infection.[39]

Each subsequent relapse of C. difficile tends to be more severe than previous infections. Long-term treatment with a vancomycin taper supplemented with probiotics, especially Saccharomyces boulardii, is associated with a higher rate of success.[54]

After three relapses, patients may be treated with oral fidaxomicin, a narrow-spectrum antibiotic. The usual dosage is 200 mg twice a day orally for 10 days. Fidaxomicin is considered to be superior to vancomycin for severe CDI.[55] The major downside of treatment with fidaxomicin is the cost of medication. A 10-day course may cost up to US$3500.[citation needed] When a patient is deteriorating or progressing to severe-complicated disease the addition of intravenous tigecycline merits considerations.[56][57] Patients with high risk of relapse may also benefit from the addition of the monoclonal antibody bezlotoxumab to the standard of care.[58]

Patients who do not respond to traditional antibiotic therapy may be eligible for a fecal microbiota transplant (FMT). Healthcare providers can transfer stool from a healthy person to the colon of a patient with recurrent CDI. This process is the most successful treatment for severe CDI with a cure rate around 93%. Fecal matter transplants have also been found to be an effective and safe treatment option for children and young adults.[59] Recurrence rates of CDI in patients treated with a FMT are generally low, around 19%, which makes it very effective at treating chronic CDI cases. However, in some cases, flares of inflammatory bowel disease are a possible side effect of the treatment.[60] The state of the host immune system is important when considering the success of  microbiota-based treatments in clearing infection.[61] Long-term effects of FMT are unknown, as the procedure has only been FDA-approved for recurrent CDI since 2013 and relatively few procedures have been performed. If transplantation is not an option, removal of the infected part of the colon can cure CDI.[55][39]

The prediction of C. difficile recurrence has been of great interest, but there has been no consensus on significantly associated risk factors.[62]

Prevention

C. difficile infection is spread through the fecal-oral route through ingestion and acid-resistant spores. Appropriate hand hygiene of healthcare workers is vital to remove spores, which includes thoroughly washing one's hands with soap and warm water. Additionally, isolation of patients with acute diarrhea can prevent the spread of spores within the hospital. [63] Another way to prevent CDIs is wearing personal protective equipment when interacting with C. difficile patients. Furthermore, CDI transmission can be prevented by daily environmental sporicidal disinfection in patient rooms. Also, reducing the length of antibiotic therapy decreases the CDI rates in hospitals.[64]

Strains

In 2005, molecular analysis led to the identification of the C. difficile strain type characterized as group BI by restriction endonuclease analysis, as North American pulse-field-type NAP1 by pulsed-field gel electrophoresis and as ribotype 027; the differing terminology reflects the predominant techniques used for epidemiological typing. This strain is referred to as C. difficile BI/NAP1/027.[49]

As of 2016, the NAP1 strain has been replaced by novel strains in some areas of British Columbia. These novel strains include NAP2 and NAP4, and some strains that do not have a NAP designation. The frequency of these novel strains increased from 2008 to 2013 in one studied region, displacing the originally more common and recognizable NAP1 bacteria.[65]

Two strains, ribotypes RT078 and RT027, can live on low concentrations of the sugar trehalose; both strains became more common after trehalose was introduced as a food additive in the early 2000s, thus increasing dietary trehalose intake.[66]

Genome

Genomic information
NCBI genome ID535
Ploidyhaploid
Genome size4.3 Mb
Number of chromosomes1
Year of completion2005

The first complete genome sequence of a C. difficile strain was published in 2005 by

Illumina.[67]

Researchers at McGill University in Montreal sequenced the genome of the highly virulent Quebec strain of C. difficile in 2005 using ultra-high throughput sequencing technology. The tests involved doing 400,000 DNA parallel-sequencing reactions of the bacterium's genome, which had been fragmented for sequencing. These sequences were assembled computationally to form a complete genome sequence.[27][68]

In 2012, scientists at University of Oxford sequenced C. difficile genomes from 486 cases arising over four years in Oxfordshire using next-generation sequencing technologies from Illumina.[69]

Epigenome

Clostridioides difficile has a highly diverse epigenome, with 17 high-quality methylation motifs reported so far, the majority pertaining to the 6mA type. Methylation at one of these motifs - CAAAAA, was shown to impact sporulation, a key step in C. difficile disease transmission, as well as cell length, biofilm formation, and host colonization.[35]

Bacteriophage

At least eight mainly temperate bacteriophages have been isolated from C. difficile, ranging in genome size from about 30 to about 60 kbp.[70] Both environmentally and clinically derived C. difficile strains carry a diverse and prevalent set of prophages.[70]

Etymology and pronunciation

See Clostridioides difficile infection § Etymology and pronunciation.

References

  1. doi:10.1001/archpedi.1935.01970020105010
    .
  2. ^ Prévot AR (1938). "Études de systématique bactérienne. IV. Critique de la conception actuelle du genre Clostridium". Annales de l'Institut Pasteur. 61 (1): 84. Archived from the original on July 28, 2020. Retrieved December 15, 2018.
  3. Deutsche Sammlung von Mikroorganismen und Zellkulturen. Archived
    from the original on November 8, 2022. Retrieved November 8, 2022.
  4. PMID 35678528
    .
  5. ^ "Johns Hopkins Doctors Discover That a Common Infection May Cause Cancer". August 24, 2022. Archived from the original on May 26, 2023. Retrieved August 29, 2022.
  6. PMID 23733223
    .
  7. PMID 34837014
    .
  8. ^
    ISBN 978-0-8385-8529-0
    .
  9. ^ "Clostridioides difficile Infection | HAI | CDC". www.cdc.gov. January 2, 2020. Archived from the original on March 8, 2020. Retrieved March 10, 2020.
  10. PMID 24066741
    .
  11. PMID 29732377
    .
  12. PMID 23478961
    .
  13. PMID 37323360
    .
  14. PMID 2387993
    .
  15. PMID 2911306
    .
  16. ^
    S2CID 211074075
    .
  17. PMID 24265690
    .
  18. PMID 28891789
    .
  19. ^
    PMID 27370902
    .
  20. PMID 29473021
    .
  21. PMID 27902180
    .
  22. PMID 27153087
    .
  23. PMID 7883950
    .
  24. PMID 15353562
    .
  25. ^ [Medical Micriobiology, Fifth Edition, Patrick Murray, Elsevier Mosby, 2005, page 412]
  26. S2CID 52297840. Archived
    from the original on July 28, 2020. Retrieved October 8, 2018.
  27. ^
    S2CID 14818750
    .
  28. PMID 16156321
    .
  29. PMID 29682562
    .
  30. PMID 31611365
    .
  31. ^ "Bleach does not kill common superbug, study finds". BBC News. November 22, 2023. Archived from the original on November 22, 2023. Retrieved November 22, 2023.
  32. PMID 38421181. Archived
    from the original on March 4, 2024. Retrieved March 4, 2024.
  33. ^ "Clostridium difficile Infection Information for Patients". Health-care Associated Infections (HAI). U.S. Centers for Disease Control and Prevention. Archived from the original on March 30, 2017. Retrieved May 22, 2017.
  34. ^ "WHO Guidelines on Hand Hygiene in Health Care: a Summary" (PDF). World Health Organization. 2009. p. 31. Archived (PDF) from the original on May 9, 2020. Retrieved June 18, 2018.
  35. ^
    PMID 31768029
    .
  36. PMID 20642351
    .
  37. ^ Belluck P (February 25, 2015). "Death Toll From C. Difficile Is Raised". The New York Times. Archived from the original on September 29, 2017. Retrieved February 25, 2015.
  38. ^ Murray P (2005). Medical Microbiology (Fifth ed.). Elsevier Mosby. p. 412.
  39. ^ a b c "Could you have deadly diarrhea (C. Diff)?". January 4, 2019. Archived from the original on March 30, 2017. Retrieved May 22, 2017.
  40. PMID 27158839
    .
  41. ^
    PMID 22752867
    .
  42. PMID 38508330
    .
  43. PMID 28765714
    .
  44. PMID 22787196
    .
  45. PMID 33253683
    .
  46. ^
    PMID 37389232
    .
  47. S2CID 231611318
    .
  48. PMID 33649048
    .
  49. ^
    S2CID 23376891
    .
  50. PMID 29462280
    .
  51. PMID 21844027
    .
  52. PMID 29053792
    .
  53. PMID 30400734
    .
  54. ^ "Treatment of Recurrent Clostridium difficile Colitis with Vancomycin and Saccharomyces boulardii" (PDF). The American Journal of Gastroenterology. Archived from the original (PDF) on August 9, 2017. Retrieved April 28, 2018.
  55. ^
    S2CID 54629762
    .
  56. S2CID 239473944
    .
  57. PMID 25982915
    .
  58. PMID 32632582
    .
  59. PMID 33520564
    .
  60. S2CID 3841585
    .
  61. PMID 33531483
    .
  62. PMID 23086451. Archived
    from the original on March 21, 2024. Retrieved March 20, 2024.
  63. PMID 24516694
    .
  64. PMID 29505879
    .
  65. PMID 27366181
    .
  66. PMID 29310122
    .
  67. PMID 20368420
    .
  68. ^ Scientists map C. difficile strain Archived May 22, 2022, at the Wayback Machine – Institute of Public Affairs, Montreal
  69. PMID 23259504
    .
  70. ^
    PMID 24808893
    .

External links
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