ADP-ribosylation

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ADP-ribose

ADP-ribosylation is the addition of one or more

gene regulation and apoptosis.[3][4]
Improper ADP-ribosylation has been implicated in some forms of cancer.[5] It is also the basis for the toxicity of bacterial compounds such as cholera toxin, diphtheria toxin, and others.[6]

History

The first suggestion of ADP-ribosylation surfaced during the early 1960s. At this time,

NAD+, as the incorporated group. Several years later, the enzymes responsible for this incorporation were identified and given the name poly(ADP-ribose)polymerase. Originally, this group was thought to be a linear sequence of ADP-ribose units covalently bonded through a ribose glycosidic bond. It was later reported that branching can occur every 20 to 30 ADP residues.[8]

The first appearance of mono(ADP-ribosyl)ation occurred a year later during a study of toxins: the diphtheria toxin of Corynebacterium diphtheriae was shown to be dependent on NAD+ in order for it to be completely effective,[9] leading to the discovery of enzymatic conjugation of a single ADP-ribose group by mono(ADP-ribosyl)transferase.

It was initially thought that ADP-ribosylation was a

post translational modification involved solely in gene regulation. However, as more enzymes with the ability to ADP-ribosylate proteins were discovered, the multifunctional nature of ADP-ribosylation became apparent. The first mammalian enzyme with poly(ADP-ribose)transferase activity was discovered during the late 1980s. For the next 15 years, it was thought to be the only enzyme capable of adding a chain of ADP-ribose in mammalian cells.[10] During the late 1980s, ADP-ribosyl cyclases, which catalyze the addition of cyclic-ADP-ribose groups to proteins, were discovered. Finally, sirtuins, a family of enzymes that also possess NAD+-dependent deacylation activity, were discovered to also possess mono(ADP-ribosyl)transferase activity.[11][12]

Catalytic mechanism

disputed – discuss
]

The source of ADP-ribose for most enzymes that perform this modification is the redox cofactor

N-glycosidic bond of NAD+ that bridges the ADP-ribose molecule and the nicotinamide group is cleaved, followed by nucleophilic attack
by the target amino acid side chain. (ADP-ribosyl)transferases can perform two types of modifications: mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation.

Mono(ADP-ribosyl)ation

N-glycosidic bond
between arginine and ribose to release ADP-ribose and unmodified protein; NAD+ is not restored by the reverse reaction.

Poly(ADP-ribosyl)ation

Main article: Poly (ADP-ribose) polymerase

Poly(ADP-ribose)polymerases (PARPs) are found mostly in

eukaryotes and catalyze the transfer of multiple ADP-ribose molecules to target proteins. As with mono(ADP-ribosyl)ation, the source of ADP-ribose is NAD+. PARPs use a catalytic triad
of His-Tyr-Glu to facilitate binding of NAD+ and positioning of the end of the existing poly(ADP-ribose) chain on the target protein; the Glu facilitates catalysis and formation of a (1''→2') O-glycosidic linkage between two ribose molecules. There are several other enzymes that recognize poly(ADP-ribose) chains, hydrolyse them or form branches; over 800 proteins have been annotated to contain the loosely defined poly(ADP-ribose) binding motif; therefore, in addition to this modification altering target protein conformation and structure, it may also be used as a tag to recruit other proteins or for regulation of the target protein.[14]

Amino acid specificity

Many different

aspartate) were described as the main sites of ADP-ribosylation. However, many other ADP-ribose acceptor sites such as serine,[16][17] arginine,[18] cysteine,[19] lysine,[20] diphthamide,[21] phosphoserine,[22] and asparagine[23]
have been identified in subsequent works.

Function

Apoptosis

During DNA damage or cellular stress PARPs are activated, leading to an increase in the amount of poly(ADP-ribose) and a decrease in the amount of NAD+.[24] For over a decade it was thought that PARP1 was the only poly(ADP-ribose)polymerase in mammalian cells, therefore this enzyme has been the most studied. Caspases are a family of cysteine proteases that are known to play an essential role in programmed cell death. This protease cleaves PARP-1 into two fragments, leaving it completely inactive, to limit poly(ADP-ribose) production. One of its fragments migrates from the nucleus to the cytoplasm and is thought to become a target of autoimmunity.

During caspase-independent

tumor necrosis factor protein. Though the mechanism is not yet understood, PARP inhibitors have been shown to affect necroptosis.[25]

Gene regulation

ADP-ribosylation can affect gene expression at nearly every level of regulation, including chromatin organization, transcription factor recruitment and binding, and mRNA processing.

The organization of

histones
.

Crystal structure of PARP1 zinc finger domain bound to DNA (purple). PDB: 4AV1

PARPs have been shown to affect transcription factor structure and cause recruitment of many transcription factors to form complexes at DNA and elicit transcription. Mono(ADP-ribosyl)transferases are also shown to affect transcription factor binding at promoters. For example, PARP14, a mono (ADP-ribosyl)transferase, has been shown to affect STAT transcription factor binding.

Other (ADP-ribosyl)transferases have been shown to modify proteins that bind

mRNA, which can cause silencing of that gene transcript.[26]

DNA repair

Poly(ADP-ribose)polymerases (PARPs) can function in DNA repair of single strand breaks as well as double strand breaks. In single-strand break repair (base excision repair) the PARP can either facilitate removal of an oxidized sugar or strand cleavage. PARP1 binds the single-strand breaks and pulls any nearby base excision repair intermediates close. These intermediates include XRCC1 and APLF and they can be recruited directly or through the PBZ domain of the APLF.[27] This leads to the synthesis of poly(ADP-ribose). The PBZ domain is present in many proteins involved in DNA repair and allows for the binding of the PARP and thus ADP-ribosylation which recruits repair factors to interact at the break site. PARP2 is a secondary responder to DNA damage but serves to provide functional redundancy in DNA repair.[28]

DNA repair facilitated by PARP1 recruitment of repair enzymes. Repair of a single strand break in DNA is initiated by the binding of PARP1. PARP1 binds single-strand breaks and pulls base excision repair intermediates close, leading to the synthesis of poly(ADP-ribose). XRCC1 is the X-ray repair cross complementing protein 1. XRCC1 complexes with polynucleotide kinase (PNK) which processes DNA termini. PCNA is the proliferating cell nuclear antigen that serves as a DNA clamp that aids in the DNA polymerase activity (DNA pol) FEN1 (Flap endonuclease 1) is then recruited to remove the overhanging 5' flap. The last step of DNA repair involves DNA ligase which brings the final DNA strands together in a phosphodiester bond.

There are many mechanisms for the repair of damaged double stranded DNA. PARP1 may function as a synapsis factor in alternative non-homologous end joining. Additionally, it has been proposed that PARP1 is required to slow replication forks following DNA damage and promotes homologous recombination at replication forks that may be dysfunctional. It is possible that PARP1 and PARP3 work together in repair of double-stranded DNA and it has been shown that PARP3 is critical for double-stranded break resolution. There are two hypotheses by which PARP1 and PARP3 coincide. The first hypothesis states that the two (ADP-ribosyl)transferases serve to function for each other's inactivity. If PARP3 is lost, this results in single-strand breaks, and thus the recruitment of PARP1. A second hypothesis suggests that the two enzyme work together; PARP3 catalyzes mono(ADP-ribosyl)ation and short poly(ADP-ribosyl)ation and serves to activate PARP1.[28]

The PARPs have many protein targets at the site of DNA damage. KU protein and DNA-PKcs are both double-stranded break repair components with unknown sites of ADP-ribosylation. Histones are another protein target of the PARPs. All core histones and linker histone H1 are ADP-ribosylated following DNA damage. The function of these modifications is still unknown, but it has been proposed that ADP-ribosylation modulates higher-order chromatin structure in efforts to facilitate more accessible sites for repair factors to migrate to the DNA damage.

Protein degradation

The ubiquitin-proteasome system (UPS) figures prominently in protein degradation. The 26S proteasome consists of a catalytic subunit (the 20S core particle), and a regulatory subunit (the 19S cap).[29] Poly-ubiquitin chains tag proteins for degradation by the proteasome, which causes hydrolysis of tagged proteins into smaller peptides.

Physiologically, PI31 attacks 20S catalytic domain of 26S Proteasome that results in decreased proteasome activity. (ADP-ribosyl)transferase Tankyrase (TNKS) causes ADP-ribosylation of PI31 which in turn increases the proteasome activity. Inhibition of TNKs further shows the reduced 26S Proteasome assembly. Therefore, ADP-ribosylation promotes 26S Proteasome activity in both Drosophila and human cells.[30]

Enzyme regulation

The activity of some enzymes is regulated by ADP-ribosylation. For instance, the activity of Rodospirillum rubrum di-nitrogenase-reductase is turned off by ADP-ribosylation of an arginine residue, and reactivated by the removal of the ADP-ribosyl group.[31]

Clinical significance

Cancer

PARP1 is involved in

NAD+ in order to perform its function in apoptosis. If a PARP becomes overactive the cell will have decreased levels of NAD+ cofactor as well as decreased levels of ATP and thus will undergo necrosis. This is important in carcinogenesis because it could lead to the selection of PARP1 deficient cells (but not depleted) due to their survival advantage during cancer growth.[32]

Susceptibility to carcinogenesis under PARP1 deficiency depends significantly on the type of DNA damage incurred. There are many implications that various PARPs are involved in preventing carcinogenesis. As stated previously, PARP1 and PARP2 are involved in BER and chromosomal stability. PARP3 is involved in

Tankyrase is another (ADP-ribosyl)polymerase that is involved in telomere length regulation.[5]

PARP1 inhibition has also been widely studied in anticancer therapeutics. The mechanism of action of a PARP1 inhibitor is to enhance the damage done by chemotherapy on the cancerous DNA by disallowing the reparative function of PARP1 in BRCA1/2 deficient individuals .

PARP14 is another ADP-ribosylating enzyme that has been well-studied in regards to cancer therapy targets; it is a signal transducer and activator of STAT6 transcription-interacting protein, and was shown to be associated with the aggressiveness of B-cell lymphomas.[32]

Bacterial toxins

Bacterial ADP-ribosylating exotoxins (bAREs) covalently transfer an ADP-ribose moiety of NAD+ to target proteins of infected eukaryotes, to yield nicotinamide and a free hydrogen ion. bAREs are produced as enzyme precursors, consisting of a "A" and "B" domains: the "A" domain is responsible for ADP-ribosylation activity; and, the "B" domain for translocation of the enzyme across the membrane of the cell. These domains can exist in concert in three forms: first, as single polypeptide chains with A and B domains covalently linked; second, in multi-protein complexes with A and B domains bound by non-covalent interactions; and, third, in multi-protein complexes with A and B domains not directly interacting, prior to processing.[6]

Crystal structure of the diphtheria toxin. PDB: 1MDT

Upon activation, bAREs ADP-ribosylate any number of eukaryotic proteins; such mechanism is crucial to the instigation of the diseased states associated with ADP-ribosylation.

Ras, and Pertussis toxin ADP-ribosylates Gi, Go, and Gt. Diphtheria toxin ADP-ribosylates ribosomal elongation factor EF-2, which attenuates protein synthesis.[6]

There are a variety of bacteria which employ bAREs in infection: CARDS toxin of

heat-labile enterotoxin of E. coli; exotoxin A of Pseudomonas aeruginosa; pertussis toxin of B. pertussis; C3 toxin of C. botulinum; and diphtheria toxin of Corynebacterium diphtheriae.[33]

See also

References

  1. PMID 17161604
    .
  2. PMID 10712584
    .
  3. S2CID 8820773
    .
  4. PMID 12727863
    .
  5. ^
    PMID 23590234
    .
  6. ^
    PMID 7704894
    .
  7. PMID 14019961
    .
  8. ^ Hayaishi, O.; Ueda, K. (2012). Poly- and Mono(ADP-ribosyl)ation Reactions: Their Significance in Molecular Biology. In ADP-Ribosylation Reactions: Biology and Medicine. New York: Academic Press.
  9. PMID 14238922
    .
  10. PMID 16959969
    .
  11. PMID 10381378
    .
  12. PMID 26166706
    .
  13. PMID 20652610
    .
  14. PMID 24970154
    .
  15. PMID 28431224
    .
  16. PMID 27723750
    .
  17. PMID 28190768
    .
  18. ^ Laing S, Koch-Nolte F, Haag F, Buck F. "Strategies for the identification of arginine ADP-ribosylation sites". Journal of proteomics. 2011;75:169–176.
  19. ^ McDonald LJ, Moss J. "Enzymatic and nonenzymatic ADP-ribosylation of cysteine". Mol Cell Biochem. 1994;138:221–226.
  20. PMID 20525793
    .
  21. ^ Oppenheimer NJ, Bodley JW. Diphtheria toxin. "Site and configuration of ADP-ribosylation of diphthamide in elongation factor 2". J Biol Chem. 1981;256:8579–8581.
  22. ^ Smith JA, Stocken LA. "Chemical and metabolic properties of adenosine diphosphate ribose derivatives of nuclear proteins". Biochem J. 1975;147:523–529.
  23. ^ Manning DR, Fraser BA, Kahn RA, Gilman AG. "ADP-ribosylation of transducin by islet-activation protein. Identification of asparagine as the site of ADP-ribosylation". J Biol Chem. 1984;259:749–756.
  24. PMID 10068897
    .
  25. PMID 24896207
    .
  26. PMID 25575290
    .
  27. PMID 11948190
    .
  28. ^
    PMID 22186780
    .
  29. PMID 19286367
    .
  30. PMID 23622245
    .
  31. PMID 3090031
    .
  32. ^ a b Boulares HA, Yakovlev AG, Smulson ME (2000). "Genome Degradation by DNAS1L3 Endonuclease: A Key PARP-1-Regulated Event in Apoptosis". Madame Curie Bioscience Database.
  33. PMID 18785839
    .

Further reading
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