Mutation breeding

Source: Wikipedia, the free encyclopedia.

Mutation breeding, sometimes referred to as "variation breeding", is the process of exposing seeds to

cultivars
. Plants created using mutagenesis are sometimes called mutagenic plants or mutagenic seeds.

From 1930 to 2014 more than 3200 mutagenic plant varieties were released[3][4] that have been derived either as direct mutants (70%) or from their progeny (30%).[5] Crop plants account for 75% of released mutagenic species with the remaining 25% ornamentals or decorative plants.[6] However, although the FAO/IAEA reported in 2014 that over 1,000 mutant varieties of major staple crops were being grown worldwide,[3] it is unclear how many of these varieties are currently used in agriculture or horticulture around the world, as these seeds are not always identified or labeled as having a mutagenic provenance.[7]

History

According to garden historian Paige Johnson:

After WWII, there was a concerted effort to find 'peaceful' uses for atomic energy. One of the ideas was to bombard plants with radiation and produce lots of mutations, some of which, it was hoped, would lead to plants that bore more heavily or were disease or cold-resistant or just had unusual colors. The experiments were mostly conducted in giant gamma gardens on the grounds of national laboratories in the US but also in Europe and countries of the [then-]USSR.[8]

Processes

There are different kinds of mutagenic breeding such as using chemical mutagens like

transposons to generate mutants. Mutation breeding is commonly used to produce traits in crops such as larger seeds, new colors, or sweeter fruits, that either cannot be found in nature or have been lost during evolution.[9]

Radiation

Exposing plants to radiation is sometimes called radiation breeding and is a sub class of mutagenic breeding. Radiation breeding was discovered in the 1920s when Lewis Stadler of the University of Missouri used X-rays on maize and barley. In the case of barley, the resulting plants were white, yellow, pale yellow and some had white stripes.[10] In 1928, Stadler first published his findings on radiation-induced mutagenesis in plants.[11] During the period 1930–2004, radiation-induced mutant varieties were developed primarily using gamma rays (64%) and X-rays (22%).[6]: 187 

Radiation breeding may take place in atomic gardens;[11] and seeds have been sent into orbit in order to expose them to more cosmic radiation.[12]

plant pathogens.[13]

Chemicals

High rates of chromosome aberrations resulting from ionizing radiation and the accompanied detrimental effects made researchers look for alternate sources for inducing mutations. As a result, an array of chemical mutagens has been discovered. The most widely used chemical mutagens are

TILLING populations.[14] Although many chemicals are mutagens, only few have been used in practical breeding as the doses need to be optimised and also because the effectiveness is not high in plants for many.[citation needed
]

Restriction endonucleases

Interest in the use of bacterial

Fok1[2] and CRISPR/Cas9[1][2] – to study double-stranded breaks in plant DNA began in the mid-nineties. These breaks in DNA, otherwise known as DSBs, were found to be the source of much chromosomal damage in eukaryotes, causing mutations in plant varieties. REs induce a result on plant DNA similar to that of ionizing radiation or radiomimetic chemicals. Blunt ended breaks in the DNA, unlike sticky ended breaks, were found to produce more variations in chromosomal damage, making them the more useful type of break for mutation breeding. While the connection of REs to chromosomal aberrations is mostly limited to research on mammalian DNA, success in mammalian studies caused scientists to conduct more studies of RE-induced chromosomal and DNA damage on barley genomes. Due to restriction endonucleases' ability to facilitate damage in chromosomes and DNA, REs have the capability of being used as a new method of mutagenesis to promote the proliferation of mutated plant varieties.[15][1][2]

Space-breeding

The ability of plants to develop and thrive is dependent on conditions such as

cosmic radiation in space. China has been experimenting with this theory by sending seeds into space, testing to see if space flights will cause genetic mutations. Since 1987, China has cultivated 66 mutant varieties from space through their space-breeding program. Chromosomal aberrations greatly increased when seeds were sent into aerospace compared to their earth-bound counterparts. The effect of space flight on seeds depends on their species and variety. For example, space-bred wheat saw a large growth in seed germination in compared to its Earth-bound control, but space-bred rice had no visible advantage compared to its control. For the varieties that were positively mutated by space flight, their growth potential exceeded that of not only their Earth-grown counterparts, but also their irradiated counterparts on Earth. Compared to traditional mutagenic techniques, space-bred mutations have greater efficacy in that they experience positive effects on their first generation of mutation, whereas irradiated crops often see no advantageous mutations in their first generations. Though multiple experiments have shown the positive effects of space flight on seed mutation, there is no clear connection as to what aspect of aerospace has produced such advantageous mutations. There is much speculation around cosmic radiation being the source of chromosomal aberrations, but so far, there has been no concrete evidence of such connection. Though China's space-breeding program has been shown to be very successful, the program requires a large budget and technological support that many other countries are either unwilling or unable to provide, meaning this program is unfeasible outside of China. Due to such restraints, scientists have been trying to replicate space condition on Earth in order to promote the same expedient space-born mutations on Earth. One such replication is a magnetic field-free space (MF), which produces an area with a weaker magnetic field than that of Earth. MF treatment produced mutagenic results, and has been used to cultivate new mutant varieties of rice and alfalfa. Other replications of space conditions include irradiation of seeds by a heavy 7 Li-ion beam or mixed high-energy particles.[16] These space-bred varieties are already being introduced to the public. In 2011, during the National Lotus Flowers Exhibition in China, a mutant lotus, called the "Outer Space Sun", was shown at the flower show.[17]

Ion beam technology

RIKEN Accelerator Research Facility, and various other Japanese institutions. During the process of ion beam radiation, seeds are wedged between two kapton films and irradiated for roughly two minutes. Mutation frequencies are notably higher for ion beam radiation compared to electron radiation, and the mutation spectrum is broader for ion beam radiation compared to gamma ray radiation. The broader mutation spectrum was revealed through the largely varied amount of flower phenotypes produced by ion beams. Flowers mutated by the ion beams exhibited a variety of colors, patterns, and shapes. Through ion beam radiation, new varieties of plants have been cultivated. These plants had the characteristics of being ultraviolet light-B resistant, disease resistant, and chlorophyll-deficient. Ion beam technology has been used in the discovery of new genes responsible for the creation of more robust plants, but its most prevalent use is commercially for producing new flower phenotypes, like striped chrysanthemums.[18]

Mature pollen treated with gamma radiation

rice cultivars, Jiaohezaozhan and Jiafuzhan, in China. Along with facilitating the creation of these two rice cultivars, the irradiation of mature rice pollen has produced roughly two hundred mutant rice lines. Each of these lines produce rice grains of both a higher quality and larger size. The mutations produced by this technique vary with each generation, meaning further breeding of these mutated plants could produce new mutations. Traditionally, gamma radiation is used on solely adult plants, and not on pollen. The irradiation of mature pollen allows mutant plants to grow without being in direct contact with gamma radiation. This discovery is in contrast to what was previously believed about gamma radiation: that it could only elicit mutations in plants and not pollen.[19]

Comparison to other techniques

In the

terms of use, patents and proposed genetic user restriction technologies and other intellectual property
regimes and modes of enforcement.

Unlike genetically modified crops, which typically involve the insertion of one or two target genes, plants developed via mutagenic processes with random, multiple and unspecific genetic changes[25] have been discussed as a concern[26] but are not prohibited by any nation's organic standards. Reports from the US National Academy of Sciences state that there is no scientific justification for regulating genetic engineered crops while not doing so for mutation breeding crops.[7]

Several organic food and seed companies promote and sell certified organic products that were developed using both chemical and nuclear mutagenesis.[27] Several certified organic brands, whose companies support strict labeling or outright bans on GMO-crops, market their use of branded wheat and other varietal strains which were derived from mutagenic processes without any reference to this genetic manipulation.[27] These organic products range from mutagenic barley and wheat ingredient used in organic beers[28] to mutagenic varieties of grapefruits sold directly to consumers as organic.[29]

Release by nation

As of 2011 the percentage of all mutagenic varieties released globally, by country, were:[6]: 187 [30]

Notable varieties per country include:

 Argentina
  • Colorado Irradiado groundnut (mutant created with X-rays; high fat content and yield, 80% of groundnuts grown in Argentina in the 1980s was Colorado Irradiado)[31]
  • Puita INTA-CL rice mutant (herbicide resistance and good yield; also grown in Bolivia, Brazil, Costa Rica and Paraguay)[31]
 Australia
  • Amaroo rice mutant variety (60–70% of rice grown in Australia was Amaroo in 2001)[31]
 Bangladesh
  • Binasail, Iratom-24 and Binadhan-6 rice mutants[31]
  • Binamoog-5 mung bean mutant variety[31]
 Cuba
  • Maybel tomato mutant (excellent drought resistance)[31]
  • GINES rice mutant (created using proton radiation; grows well in salty conditions)[31]
 People's Republic of China
  • Henong series soybean mutants[31]
  • Jiahezazhan and Jiafuzhan rice (mutations obtained by pollen irradiation; high yield and quality, very adaptable, resistant to plant hopper and blast)[31]
  • Lumian Number 1 cotton[32]
  • Purple Orchard 3 Sweet potato[33]
  • Tiefeng 18 soybean[31]
  • Yangdao Number 6 rice[32]
  • Yangmai 156 wheat[32]
  • Zhefu 802 rice mutant (irradiated with gamma rays; resistant to rice blast, good yield even in poor conditions, the most planted rice variety between 1986–1994)[34]
  • 26Zhaizao
    indica rice mutant (created with gamma rays)[34]

 Czech Republic

  • Diamant barley (high yield, short height mutant created with X-Rays)[35]
 Egypt
  • Giza 176 and Sakha 101 high yield rice mutants[31]
 Finland
  • Balder J barley mutant (better drought resistance, yield and sprouting)[31]
  • Puhti and Ryhti stiff straw oat mutants[31]
 France
  • High oleic sunflowers (covering more than 50 % of the sunflower acreage)
 Germany
 Ghana
  • Tek bankye mutant cassava (good poundability and increased dry matter content)[31]
 India
 Italy
  • Durum wheat (especially Creso mutant, created with thermal neutrons)[36][37]
 Japan
  • Osa Gold Pear (disease resistance)[38]
  • Most rice varieties grown in Japan have the sd1 mutant allele from the Reimei rice variety[32]
 Myanmar
  • Shwewartun rice mutant (created by irradiating IR5 rice to give better yield, grain quality and earlier maturity)[31]
 Pakistan
  • Basmati 370 short height rice mutant[34]
  • NIAB-78 cotton mutant (high yielding, heat tolerant, early maturing)[34]
  • CM-72 chickpea mutant (created with 150Gy of gamma rays; high yielding, blight resistant)[39]
  • NM-28
    mungbean mutant (short height, uniform and early maturing, high seed yield)[39]
  • NIAB Masoor 2006 lentil mutant (created with 200Gy of radiation; early maturing, high yield, resistant to disease)[39]
 Peru
  • UNA La Molina 95 barley mutant (developed in 1995 for growing above 3,000 m)[40]
  • Centenario
    Amarinth "kiwicha" mutant (high quality grain and exported as a certified organic product)[40]
  • Centenario II barley mutant (developed for growing in the Andean highlands with high yield, high quality flour and tolerance to hail)[40]
 Sudan
  • Albeely banana mutant (better quality, high yield and better stand)[31]
 Thailand
  • RD15 and RD6 aromatic indica rice mutants (created with gamma rays and released in 1977-8; RD 15 is early ripening, RD6 has a valuable glutinous endosperm). Thailand is the biggest exporter of aromatic rice in the world[31]
 United Kingdom
  • Golden Promise barley (semi-dwarf, salt tolerant mutant created with gamma rays)[41] Is used to make beer and whisky[42]
 United States
 Vietnam
  • VND 95-20, VND-99-1 and VN121 rice mutants (increased yield, improved quality, resistance to disease and pests)[43][44]
  • DT84, DT96, DT99 and DT 2008 soybean mutants (developed using gamma rays to grow three crops a year, tolerance to heat and cold and resistance to disease)[44]

In 2014, it was reported that 17 rice mutant varieties, 10 soybean, two maize and one chrysanthemum mutant varieties had been officially released to Vietnamese farmers. 15% of rice and 50% of soybean was produced from mutant varieties.[45]

See also

References

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  21. ^ The Canadian regulatory system is based on whether a product has novel features regardless of method of origin. In other words, a product is regulated as genetically modified if it carries some trait not previously found in the species whether it was generated using mutation breeding or genetic engineering (or any other method including selective breeding).
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  40. ^ a b c (2012) Improved barley varieties – Feeding people from the equator to the arctic Archived 2014-08-29 at the Wayback Machine Joint FAO/IAEAProgramme, Nuclear Techniques in Food and Agriculture, Retrieved 25 October 2013
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  43. ^ (2012) Successful Mutation Breeding Programmes in Vietnam Archived 2013-10-29 at the Wayback Machine Joint FAO/IAEAProgramme, Nuclear Techniques in Food and Agriculture, Retrieved 25 October 2013
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External links

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