Food microbiology
Food safety |
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Terms |
Critical factors |
Bacterial pathogens |
Viral pathogens |
Parasitic pathogens |
Food microbiology is the study of the
Subgroups of bacteria that affect food
In the study of bacteria in food, important groups have been subdivided based on certain characteristics. These groupings are not of taxonomic significance:[5]
- Lactic acid bacteria are bacteria that use carbohydrates to produce lactic acid. The main genera are Lactococcus, Leuconostoc, Pediococcus, Lactobacillus and Streptococcus thermophilus.
- Acetic acid bacteria like Acetobacter aceti produce acetic acid.
- Bacteria such as Propionibacterium freudenreichii that produce propionic acid are used to ferment dairy products.
- Some Clostridium spp. Clostridium butyricum produce butyric acid.
- Proteolytic bacteria proteinases. This group includes bacteria species from the Micrococcus, Staphylococcus, Bacillus, Clostridium, Pseudomonas, Alteromonas, Flavobacterium and Alcaligenes genera, and more limited from Enterobacteriaceae and Brevibacterium.
- Lipolytic bacteria hydrolyze lipases. This group includes bacteria species from the Micrococcus, Staphylococcus, Pseudomonas, Alteromonas and Flavobacterium genera.
- Saccharolytic bacteria hydrolyze complex carbohydrates. This group includes bacteria species from the Bacillus, Clostridium, Aeromonas, Pseudomonas and Enterobactergenera.
- Thermoduric bacteria, including spores, can survive pasteurization. Bacteria that grow in cold temperatures below 5 Celsius are called psychotropic and include bacteria species from many genera including Alcaligenes, Serratia, Leuconostoc, Carnobacterium, Brochothrix, Listeria and Yersinia.
- Halotolerant bacteria can survive high salt concentrations greater than 10%. This includes some species from Vibrio and Corynebacterium. Aciduric bacteria survive at low pH.
- Osmophilic bacteria, while less osmophilic than yeasts and molds, can tolerate a relatively higher osmotic environment. Aerobesrequire oxygen, while anaerobes are inhibited by it. Facultative anaerobes can grow with and without oxygen.
- Some bacteria can produce gases during metabolism of nutrients, others produce slime by synthesizing polysaccharides.
- Spore producing bacteria are further divided into subgroups of aerobic, anaerobic, flat sour, thermophilic and sulfide-producing.
- Coliforms, including fecal coliforms(such as e.coli) are used as a measure of sanitation. Enteric pathogens can cause gastrointestinal infection and may be included in this group.
Food safety
Fermentation
Microbial biopolymers
Several microbially produced
Alginate
Poly-γ-glutamic acid
Poly-γ-glutamic acid (γ-PGA) produced by various strains of Bacillus has potential applications as a thickener in the food industry.[9]
Food testing
To ensure safety of food products, microbiological tests such as testing for pathogens and spoilage organisms are required. This way the risk of contamination under normal use conditions can be examined and food poisoning outbreaks can be prevented. Testing of food products and ingredients is important along the whole supply chain as possible flaws of products can occur at every stage of production.[10] Apart from detecting spoilage, microbiological tests can also determine germ content, identify yeasts and molds, and Salmonella. For Salmonella, scientists are also developing rapid and portable technologies capable of identifying unique variants of Salmonella.[11]
Polymerase chain reaction (PCR) is a quick and inexpensive method to generate numbers of copies of a DNA fragment at a specific band ("PCR (Polymerase Chain Reaction)," 2008). For that reason, scientists are using PCR to detect different kinds of viruses or bacteria, such as HIV and anthrax based on their unique DNA patterns. Various kits are commercially available to help in food pathogen nucleic acids extraction,[12] PCR detection, and differentiation.[13] The detection of bacterial strands in food products is very important to everyone in the world, for it helps prevent the occurrence of food borne illness. Therefore, PCR is recognized as a DNA detector in order to amplify and trace the presence of pathogenic strands in different processed food.[citation needed]
See also
- Anisakis
- Baker's yeast
- Campylobacter
- Cysticercosis
- Environmental microbiology
- Escherichia coli
- Food safety
- Foodborne illness
- Fungal infection
- List of microorganisms used in food and beverage preparation
- Microbiology
- One Health
- Shigella
- Trichinosis
- Toxoplasmosis
- Yeast infection
- Zoonosis
References
- ISBN 978-1-904455-00-4.
- ISBN 978-1-904455-01-1.
- ISBN 978-1-904455-41-7.
- ISBN 978-1-904455-68-4.
- ^ Ray, B. Fundamental Food Microbiology, 3rd Ed. (2005), pp 29-32
- PMID 23316235.
- ISBN 978-1-904455-36-3.
- ISBN 978-1-904455-36-3.
- ISBN 978-1-904455-36-3.
- ^ "Food Testing Laboratories". Archived from the original on 2011-10-20. Retrieved 2012-04-18.
- ^ "Rapid Testing and Identification of Salmonella in Foods". Archived from the original on 2022-03-27. Retrieved 2012-04-18.
- ^ "FOOD PATHOGEN DNA EXTRACTION filter paper card". Archived from the original on 2021-11-27. Retrieved 2014-07-11.
- ^ "Microbial Detection Identification Kits". Archived from the original on 2014-07-15. Retrieved 2014-07-11.